The invention discloses a blood-sucking leech larva complete formula feed and a preparation method thereof, and belongs to the technical field of blood-sucking leech breeding feed preparation. The complete formula feed is prepared from, by weight, 0.1-20% of blood cell protein powder, 0.1-20% of plasma protein powder, 0.1-10% of animal fat, 0.001-0.1% of arginine, 0.01-5% of vitamin C, 0.001-0.1% of compound vitamin premix feed, 0.2-1.0% of sodium chloride and other raw materials. After being fully dissolved with deionized water, the raw materials are crushed for 5-60 minutes at the temperature of 30-70 DEG C by using ultrasonic waves, a pig casing is filled with the product, and the feed is obtained. Protein, fat, vitamin, inorganic salt and other nutrients in the blood-sucking leech larva complete formula feed are balanced in proportion, the feed formula and the preparation technology are scientific and reasonable, the method is simple and easy to implement, the prepared complete formula feed is good in flowability, has good food calling performance and palatability on blood-sucking leeches and is high in digestion and absorption efficiency, and the blood-sucking leeches fed with the feed are high in survival rate and high in growth speed.