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Hemoglobin degrading bacterium NJM4

A hemoglobin and bacteria-degrading technology, which is applied in the direction of bacteria, microorganisms, animal feed, etc., can solve the problems of soaring prices and shortage of feed protein resources, and achieve the effects of protecting the environment, promoting sustainable development, and broad application prospects

Inactive Publication Date: 2008-12-17
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a serious shortage of feed protein resources. For example, the world production of the most important feed protein-fishmeal has been declining in recent years, and the price has been soaring.
Therefore, it is imminent to develop new feed protein as a substitute for fish meal, and it is one of the best ways to use high-efficiency strains of livestock and poultry slaughter blood as a high-quality protein feed source after fermentation.

Method used

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  • Hemoglobin degrading bacterium NJM4

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Experimental program
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Effect test

Embodiment 1

[0017] Embodiment 1: Efficient strain isolation

[0018] A total of 2 g of samples were collected from special environments such as the sewage ditch of the slaughterhouse, added to 10 mL of nutrient broth, shaken for 1 min, and allowed to stand for 5 min. The supernatant was drawn to inoculate the nutrient broth medium, and cultured at 37°C for 48 h.

[0019] Streak inoculation of the above-mentioned cultured strains on a blood agar medium plate, and culture at 37° C. for 24 hours. The formula of the blood agar medium is as follows: 1L of distilled water contains: 10.0g of peptone, 3.0g of beef extract, 5.0g of sodium chloride, and 20.0g of agar powder. Heat to dissolve, adjust pH to 7.5, and autoclave at 121°C for 15 minutes. Cool to 45°C-50°C, and add 50mL of fresh sheep blood.

[0020] Purely culture the bacteria that produce larger hemolytic circles in the above medium. A strain of bacteria with greater hemolytic ability was obtained and named: NJM4. The strain NJM4 is...

Embodiment 2

[0021] Embodiment 2: the expansion culture of above-mentioned bacterial strain NJM4

[0022] The formula of the seed medium is that 1L of distilled water contains: 6.0g of peptone, 4.0g of beef extract, 2.0g of yeast extract, and 5.0g of sodium chloride. Heat to dissolve, adjust pH to 7.5, and autoclave at 121°C for 15 minutes.

[0023] The bacterial strain NJM4 obtained above was inoculated into a 250mL Erlenmeyer flask containing 50mL of seed medium, and cultured on a shaker at a speed of 120r / min for 24h at a temperature of 37°C to obtain a NJM4 liquid culture. The number of bacteria was 4×10 11 CFU / mL.

Embodiment 3

[0024] Example 3: Using strain NJM4 to ferment and degrade hemoglobin in the hemoglobin fermentation medium Take 6 mL of the NJM4 liquid culture obtained in Example 2 and inoculate it in a 250 mL Erlenmeyer flask containing 150 mL of the hemoglobin fermentation medium at a temperature of 37°C. Cultivate for 24 h on a shaker at a speed of 120 r / min. The formula of hemoglobin fermentation medium is: 1L distilled water contains: 4.0g glucose, 0.4g dipotassium hydrogen phosphate, 0.5g sodium chloride, 0.6g potassium dihydrogen phosphate, 0.1g magnesium sulfate, 0.02g ferric sulfate, adjust pH7. Autoclave at 0,121°C for 15 minutes. Cool to 45°C-50°C, add 10.0g fresh sheep hemoglobin. The degradation rate of hemoglobin was measured by Kjeldahl method, and the degradation rate of hemoglobin was 53.6% after 24 hours of culture.

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Abstract

The invention relates to a hemoglobin degrading bacterium NJM4 which pertains to the field of high biotechnology. The classification name of the strain NJM4 is Bacillus pumilus with the strain accession number of CGMCC NO.2337. The strain is inoculated into a seed culture medium and cultured for 24h by 120r / min at the temperature of 37 DEG C to obtain the liquid NJM4 culture. The strain produces a transparent hemolysis cycle on a blood agar culture medium, the degradation rate of hemoglobin in a hemoglobin fermentation medium is 53.6 percent and the strain degrades the hemoglobin in a hemoglobin solution into micromolecular polypeptide. The degrading bacterium utilizes the method of microorganism fermentation to degrade the hemoglobin, is suitable for the production of high-quality protein feed and can be applied to the development and utilization of the waste blood resources of livestock and poultry slaughtering, especially can transform blood proteins into feed, therefore, not only changing trash to treasure and protecting environment, but also promoting sustainable development and having wide application prospect.

Description

technical field [0001] The invention relates to a hemoglobin-degrading bacterium NJM4, which belongs to the high-tech field of biology, uses microorganism fermentation to degrade hemoglobin, and is suitable for the production of high-quality protein feed. Background technique [0002] Livestock blood is a valuable protein resource. On the one hand, it is because the protein content of blood meal is very high. For example, protein in pig blood accounts for 17-20% of fresh blood. In the No. 32 report recommending the development and utilization of blood meal, the Ministry of Agriculture of the Food and Agriculture Organization of the United Nations combined blood meal with bone meal, meat meal, fish meal, Animal scraps were compared in detail, and the results showed that blood meal had the highest protein content. Moreover, all essential amino acids are available; on the other hand, the special nutritional value of the blood meal is related to the high iron content, which is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/04C12R1/08A23K10/24
Inventor 杨德吉姚大伟王政陶艳华郭士兵
Owner NANJING AGRICULTURAL UNIVERSITY
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