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Method for extracting modified plasma protein powder and bioactive peptide for enriching blood from pig blood

A plasma protein powder and active peptide technology, which is applied to the protein components of blood, protein food processing, animal protein processing, etc., can solve the problems of only 30% digestion and absorption rate, difficult to achieve industrialization, and low blood technology level. To achieve the effect of time-saving, full utilization, fine and uniform texture of the process method

Inactive Publication Date: 2010-07-07
天津市食品工业生产力促进中心
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AI Technical Summary

Problems solved by technology

[0003] At present, the technical level of blood utilization in our country is relatively low, and the products are monotonous. The main products are dried blood meal and fermented blood meal feed. The rate is very low, and the production environment needs to be improved
Although a lot of basic work has been done on the utilization of pig blood resources in China, it is still in the stage of experimental research. For example, the acid hydrolysis method is used in the hydrolysis of pig blood protein, and all of them are extracted from a single product.
In the field of food application, there are also research reports at present. The technical difficulty lies in the re-pollution problem caused by the large-scale use of organic solvents. Due to these reasons, it is difficult to achieve industrialization for the comprehensive utilization of pig blood resources.

Method used

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  • Method for extracting modified plasma protein powder and bioactive peptide for enriching blood from pig blood
  • Method for extracting modified plasma protein powder and bioactive peptide for enriching blood from pig blood

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Embodiment Construction

[0018] A method for extracting modified plasma protein powder and blood-enriching active peptide from pig blood, comprising the following steps:

[0019] 1. Preparation of hemoglobin (HC) and plasma.

[0020] Add human sodium citrate 0.33% (w / v) in the fresh pig blood, to prevent blood coagulation, then it is at 5 ℃, 3000 * g centrifugation 20min, after separating supernatant, obtain red blood cell its protein content is (33.12 Soil 2.57) g / 100g, then use a homogenizer to crush red blood cells under a pressure of 60MPa, dissolve and homogenize hemoglobin, and freeze for future use. The supernatant is plasma, which accounts for about 60% of the whole blood, and is stored at 1°C to 2°C.

[0021] 2. Biomodify the separated plasma to make modified plasma protein powder. Its process is: (1) adding transglutaminase in blood plasma to improve the gelation of plasma protein; the conditions for improving the gelation of plasma protein are: the addition of transglutaminase 5u / g, pH8 ...

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Abstract

The invention discloses a method for extracting modified plasma protein powder and bioactive peptide for enriching blood from pig blood, comprising the following steps of: taking fresh and healthy pig blood as a raw material; carrying out anticoagulation treatment, centrifuging and separation on the fresh and healthy pig blood to obtain a blood cell and blood plasma; carrying out high-pressure cutting and wall breaking, complex enzyme hydrolysis and flashing and deodorizing process treatment on the blood cell to obtain enzymolysis liquid; carrying out rough filtration, fine filtration, condensation treatment and spray drying to obtain the bioactive peptide for enriching the blood; and carrying out modification treatment on the separated blood plasma to obtain the plasma protein powder. The invention has the following advantages that: 1. the production cost is low and no pollution to the environment is generated; 2. the average molecular weight of small peptide of the produced bioactive peptide for enriching the blood is not more than 10,000Dalton, the content of free amino acid is not more than 15.0percent and the protein of the plasma protein powder is not less than 90.0 percent; 3. the water content of the produced modified plasma protein is not more than 5.0 percent, the viscosity (5 percent solution) is not less than 2.0 mpa.s and the content of the protein is not less than 90.0 percent; and 4. the process method saves time and energy, has simple operation and fully utilizes pig blood resources.

Description

Technical field: [0001] The invention relates to a method for extracting modified plasma protein powder and blood-enriching active peptide from pig blood. Background technique: [0002] According to relevant literature reports, the development of livestock and poultry blood at home and abroad began at the beginning of last century. It was mainly used in the feed industry in the early days. With the development of technology, food experts in Germany, Denmark and other countries use pig blood to separate plasma as a tonic, and add it to sausages, puddings, and pastries; in the 1970s, Sweden and France began to use fresh pig blood in sausages and puddings, and A small amount of pig blood plasma is used in products such as cakes, biscuits, bread, and dried noodles to replace egg whites, but only 25% to 30% of pig blood resources are utilized. Pig blood has also been used as an adhesive for furniture, as a clarifying agent in the brewing industry, and as raw materials to extrac...

Claims

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Application Information

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IPC IPC(8): A23J1/06A23J3/12A23J3/34A23L1/312A23L1/305A23L33/17A23L33/18
Inventor 赵丽陈树生杨萍刘元
Owner 天津市食品工业生产力促进中心
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