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Method for preparing nitroso hemoglobin pigment easy to disperse and high in thermal stability

A technology with high thermal stability and hemoglobin, applied in food science and other fields, can solve problems such as unimproved stability, poor thermal stability, and unstable color, and achieve the effects of improving poor dispersion, improving stability, and simple and easy-to-obtain raw materials

Inactive Publication Date: 2016-07-13
丁玉琴
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem mainly solved by the present invention: in view of the poor thermal stability of the current nitrosohemoglobin in the preparation process, precipitation occurs immediately after heating at 75°C, and it is no longer soluble in water, and the nitrosohemoglobin pigment is in the air or under light conditions. And the color of the meat products added with this pigment is unstable and fades quickly. Even after microcapsule embedding, the stability is not improved and the dispersibility is poor. It provides a kind of raw material that uses bovine blood as a raw material, through purification and removal of impurities. The traditional nitrosohemoglobin pigment is prepared, and pueraria root is used as raw material by hammering, and the pueraria starch suspension is collected by washing, and the starch slurry, hemoglobin pigment and amino acid are compounded after being collected by standing, heat-treated, coated, freeze-dried and pulverized, so that Preparation method for obtaining easily dispersible high thermal stability nitrosohemoglobin pigment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0020] First, collect 1L of fresh cow blood from the slaughterhouse and add 0.8% hirudin in the total volume of cow blood as an anticoagulant, place it on a magnetic stirrer and stir at a speed of 500r / min for 60min, then transfer it to a horizontal centrifuge, and centrifuge at a speed of 3000r / min After 10 minutes, separate and remove the supernatant to collect the bovine blood cells in the lower layer; then mix the bovine blood cells obtained above with deionized water at a volume ratio of 1:4, stir slowly with a glass rod for 10 minutes, put it into a 2L beaker, and then move the beaker into a water bath pot, heat up to 50°C, continue to keep warm and stir for 20 minutes to remove impurity proteins, and finally centrifuge the stirred solution at a speed of 4000r / min for 20 minutes to separate the supernatant, which is the bovine hemoglobin solution; then measure 500mL of the above-mentioned separated Put the bovine hemoglobin solution into a three-neck flask equipped with a...

example 2

[0023] First, collect 1.5L of fresh cow blood from the slaughterhouse and add 0.9% hirudin in the total volume of cow blood as an anticoagulant, place it on a magnetic stirrer and stir it at 550r / min for 80min, then transfer it to a horizontal centrifuge, and add it at a speed of 3300r / min Centrifuge for 13 minutes, separate and remove the supernatant to collect the bovine blood cells in the lower layer; then mix the above-obtained bovine blood cells and deionized water at a volume ratio of 1:4, stir slowly with a glass rod for 13 minutes, put them into a 2L beaker, and then move the beaker into In a water bath, heat up to 55°C, continue to heat and stir for 25 minutes to remove impurity proteins, and finally centrifuge the stirred solution at a speed of 4500r / min for 23 minutes to separate the supernatant, which is the bovine hemoglobin solution; then measure 550mL of the above separation to obtain Put the bovine hemoglobin solution into a three-neck flask with a stirrer and a...

example 3

[0026] First, collect 2L of fresh cow blood from the slaughterhouse and add 1.0% hirudin in the total volume of cow blood as an anticoagulant, place it on a magnetic stirrer and stir at 600r / min for 90min, then transfer it to a horizontal centrifuge, and centrifuge at 3500r / min After 15 minutes, separate and remove the supernatant to collect the bovine blood cells in the lower layer; then mix the bovine blood cells obtained above with deionized water at a volume ratio of 1:4, stir slowly with a glass rod for 15 minutes, put it into a 2L beaker, and then move the beaker into a water bath pot, heated to 60°C, continued to heat and stir for 30 minutes to remove impurity proteins, and finally centrifuged the stirred solution at a speed of 5000r / min for 25 minutes to separate the supernatant, which was the bovine hemoglobin solution; then measure 600mL of the above-mentioned separated Put the bovine hemoglobin solution into a three-neck flask equipped with a stirrer and a thermomete...

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Abstract

The invention discloses a method for preparing nitroso hemoglobin pigment easy to disperse and high in thermal stability, and belongs to the technical field of nitroso hemoglobin pigment preparation. The method comprises the following steps: by taking ox blood as a raw material, purifying and decontaminating so as to prepare conventional nitroso hemoglobin pigment, further by taking a kudzuvine root as a raw material, crushing with a hammer, washing, collecting a kudzuvine root starch turbid liquid, standing, collecting starch slurry, compounding with hemoglobin pigment and amino acid, performing thermal treatment, wrapping, and performing freeze-drying crushing, thereby obtaining the nitroso hemoglobin pigment easy to disperse and high in thermal stability. Practical tests show that the method disclosed by the invention is unique and novel; when nitroso hemoglobin is used as a substitute of nitrite, not only is the residual amount of nitrite in a meat product reduced and is damage to human bodies reduced, but also a livestock blood resource can be sufficiently utilized, the blood resource can be developed and utilized, the iron content is increased, low-saltpetre or saltpeter-free meat products can be made indeed, and the food security is improved.

Description

technical field [0001] The invention discloses a preparation method of easily dispersible and highly thermally stable nitrosohemoglobin pigment, which belongs to the technical field of nitrosohemoglobin pigment preparation. Background technique [0002] my country's meat food industry is an emerging industry developed since the reform and opening up, and occupies an important position in the food industry. According to statistics, my country's total meat output has reached 62 million tons per year, ranking first in the world and accounting for the world's meat. 28.9% of the total. At present, the average meat production in the world accounts for 10% to 20% of the meat production, and the meat production in some developed countries accounts for 60% to 70% of the total meat production, but there is still a big gap between my country and this ratio. There are only more than 2 million tons of deep-processed meat products in my country every year, accounting for 4% of the total m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/43A23L13/40A23L13/60A23L29/30A23L33/17
CPCA23V2002/00
Inventor 丁玉琴张帆林大伟
Owner 丁玉琴
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