Method for preparing nitroso hemoglobin pigment easy to disperse and high in thermal stability
A technology with high thermal stability and hemoglobin, applied in food science and other fields, can solve problems such as unimproved stability, poor thermal stability, and unstable color, and achieve the effects of improving poor dispersion, improving stability, and simple and easy-to-obtain raw materials
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example 1
[0020] First, collect 1L of fresh cow blood from the slaughterhouse and add 0.8% hirudin in the total volume of cow blood as an anticoagulant, place it on a magnetic stirrer and stir at a speed of 500r / min for 60min, then transfer it to a horizontal centrifuge, and centrifuge at a speed of 3000r / min After 10 minutes, separate and remove the supernatant to collect the bovine blood cells in the lower layer; then mix the bovine blood cells obtained above with deionized water at a volume ratio of 1:4, stir slowly with a glass rod for 10 minutes, put it into a 2L beaker, and then move the beaker into a water bath pot, heat up to 50°C, continue to keep warm and stir for 20 minutes to remove impurity proteins, and finally centrifuge the stirred solution at a speed of 4000r / min for 20 minutes to separate the supernatant, which is the bovine hemoglobin solution; then measure 500mL of the above-mentioned separated Put the bovine hemoglobin solution into a three-neck flask equipped with a...
example 2
[0023] First, collect 1.5L of fresh cow blood from the slaughterhouse and add 0.9% hirudin in the total volume of cow blood as an anticoagulant, place it on a magnetic stirrer and stir it at 550r / min for 80min, then transfer it to a horizontal centrifuge, and add it at a speed of 3300r / min Centrifuge for 13 minutes, separate and remove the supernatant to collect the bovine blood cells in the lower layer; then mix the above-obtained bovine blood cells and deionized water at a volume ratio of 1:4, stir slowly with a glass rod for 13 minutes, put them into a 2L beaker, and then move the beaker into In a water bath, heat up to 55°C, continue to heat and stir for 25 minutes to remove impurity proteins, and finally centrifuge the stirred solution at a speed of 4500r / min for 23 minutes to separate the supernatant, which is the bovine hemoglobin solution; then measure 550mL of the above separation to obtain Put the bovine hemoglobin solution into a three-neck flask with a stirrer and a...
example 3
[0026] First, collect 2L of fresh cow blood from the slaughterhouse and add 1.0% hirudin in the total volume of cow blood as an anticoagulant, place it on a magnetic stirrer and stir at 600r / min for 90min, then transfer it to a horizontal centrifuge, and centrifuge at 3500r / min After 15 minutes, separate and remove the supernatant to collect the bovine blood cells in the lower layer; then mix the bovine blood cells obtained above with deionized water at a volume ratio of 1:4, stir slowly with a glass rod for 15 minutes, put it into a 2L beaker, and then move the beaker into a water bath pot, heated to 60°C, continued to heat and stir for 30 minutes to remove impurity proteins, and finally centrifuged the stirred solution at a speed of 5000r / min for 25 minutes to separate the supernatant, which was the bovine hemoglobin solution; then measure 600mL of the above-mentioned separated Put the bovine hemoglobin solution into a three-neck flask equipped with a stirrer and a thermomete...
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