Kit for chemiluminescent quantitative immunoassay of free beta-human chorionic gonadotrophin (hCG) and preparation method thereof

A chorionic gonadotropin and chemiluminescence immunological technology, applied in the field of immunoassay medicine, can solve the problems of high cost and low sensitivity, and achieve the effects of low cost, high sensitivity and strong specificity
CN102749461AActive Publication Date: 2012-10-24BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
Publication Date
2012-10-24

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Abstract

The invention discloses a kit for chemiluminescent quantitative immunoassay of a free beta-human chorionic gonadotrophin (hCG). The kit comprises a free beta-hCG antibody-coated plate, a free beta-hCG antibody enzyme conjugate, a free beta-hCG calibrator, a free beta-hCG quality control material, a washing liquid concentrated by 20 times, a light-emitting solution A and a light-emitting solution B. The kit has the advantages of high detection sensitivity, low cost and simple and fast operation, and has a high clinical application value. The invention also discloses a preparation method of the kit.
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Description

technical field

[0001] The invention belongs to the field of immunoanalysis medicine, in particular to a free β-hCG chemiluminescent immunoquantitative detection kit and a preparation method thereof, which can be used for quantitative determination of free β-human villi in human serum and plasma Membrane gonadotropin content. Background technique

[0002] Human chorionic gonadotropin (hCG) is a glycoprotein hormone secreted by the trophoblast cells of the placenta. It consists of two non-covalently bonded glycoprotein subunits, subunit and β subunit. The subunits are shared by the anterior pituitary hormones, while the β subunit is unique to HCG. There will be no cross-reaction with FSH, LH and TSH, etc. Although the structure is similar to β-LH, the 24 amino acids at the end are not found in β-LH, and the β-subunit is specific to human chorionic gonadotropin of. Free β-human chorionic gonadotropin (free β-hCG) is the β subunit of HCG in an unbound state, which is of grea...

Claims

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