44 results about "Iodophenol" patented technology

The invention discloses a luminescent substrate, a use of the luminescent substrate and a detection kit containing the luminescent substrate. The luminescent substrate has high sensitivity and high stability. The luminescent substrate comprises a solution A and a solution B. The solution A comprises 0.5-8mmol / L of luminal, 0.5-10mmol / L of p-lodophenol, and 0.1mol / L of a Tris-HCl buffer solution. The solution B comprises 1-10mmol / L of urea peroxide and 0.1mol / L of the Tris-HCl buffer solution. The kit comprises a monoclonal antibody-coated microporous plate, HRP-labeled monoclonal antibodies, a freeze-dried standard, a sample diluent, a concentrated washing liquid and the chemiluminescent substrate.

The invention discloses a photodynamic treatment medical composition which consists of a photosensitizer and a chemical activator, wherein the photosensitizer is expressed by the formula I; the chemical activator comprises luminol, p-iodophenol, horse radish peroxidase and hydrogen peroxide; and the proportion of the photosensitizer expressed by the formula I, to the luminol to the p-iodophenol to the horse radish peroxidase to the hydrogen peroxide in the composition is 8-12muM:0.4-0.7mM:1-1.5mM:0.01-0.02mg / mL:2-3mM. In the composition of the invention, active oxygen generated by bioluminescence energy transfer between the photosensitizer expressed by the formula I and the luminol is utilized to kill cells and fungi. Compared with the traditional photodynamic treatment method, the composition can be used for treating in deep tissues; and damages to normal tissues, which are caused by long-time irradiation by an external light source, can be avoided; and meanwhile, the composition has a profound influence on the aspects of the clinic treatment of tumors and pathogenic bacteria infection.

The invention discloses chemiluminescent substrate liquid, which is mainly composed of radiant agent luminol, reinforcing agent p-Iodophenol, oxidant urea peroxide, and is applied to a detection system taking HRP as enzymatic reaction. The chemiluminescent substrate liquid has the characteristics of short reaction time, long detection platform period, good stability and the like, and can enhance the sensitivity of a kit, broaden linear range and prolong the period of validity of the kit.

The present invention relates to the synthesis process of 2-methoxyl-5-iodophenol. The synthesis process includes the following steps: the reaction of 2-methoxyl-phenol and p-toluenesulfonyl chloride in triethylamine solvent to prepare p-toluenesulfo-(2-methoxyl) phenol ester; the reaction of p-toluenesulfo-(2-methoxyl) phenol ester, metal chloride and iodine monochloride to produce great amount of solid; suction filtering, washing with glacial acetic acid and water washing to obtain p-toluenesulfo-(2-methoxyl-5-iodo) phenol ester; and final deprotection of p-toluenesulfo-(2-methoxyl-5-iodo) phenol ester to obtain 2-methoxyl-5-iodophenol. The present invention has the advantages of simple operation, low material cost, high yield, high product purity, etc. and is suitable for industrial production.

The invention discloses a vascular endothelial growth factor quantitative determination kit and a light-emitting substrate solution thereof. The light-emitting substrate solution comprises a light emitting solution A and a light emitting solution B, wherein the light emitting solution A comprises the following ingredients with concentrations: 0.40-0.50 g / L luminol, 0.10-0.15 g / L p-iodophenol, 0.10-0.15 g / L 1,2-cyclohexanediamine tetraacetic acid, 4.60-5.40 g / L boric acid, 11.00-12.00 g / L borax, and 10.00-12.00 g / L sodium chloride; the light emitting solution B comprises the following ingredients with concentrations: 0.20-0.25 g / L urea peroxide, 1.00-1.50 ml / L Tween 20, 4.60-5.40 g / L boric acid, 11.00-12.00 g / L borax, and 10.00-12.00 g / L sodium chloride; a volume ratio of the light emitting solution A to the light emitting solution B during use is 1:1. The light-emitting substrate solution disclosed by the invention has the advantages of good stability, high sensitivity, and long photon strength platform period.

Photodynamic therapy-based methods of treating diseases such as cancer and malaria are disclosed. These methods include administering 5-aminolevulinic acid (ALA), luminol and artemisinin (ART). ART and ALA administered in combination with luminol can kill malaria parasites without host toxicity. In some aspects, the methods further include administration of a luminol enhancer such as 4-iodophenol. The disclosed methods can also be used to remove potential malaria pathogens from the blood supply.

The invention discloses stable HRP (horse radish peroxidase) chemiluminescence substrate solution which comprises luminescence agent solution and oxidant solution. The comprises luminescence agent solution is prepared by specific proportion of Luminol or Luminol derivative or Luminol sodium salt, p-Iodophenol, phenolphthalein and buffer solution, the p-Iodophenol serves as reinforcing agents, the phenolphthalein serves as synergistic reinforcing agents, the oxidant solution is prepared by specific proportion of urea peroxide, ethylene diamine tetraacetic acid, tween-20 and buffer solution, and the luminescence agent solution and the oxidant solution are mixed before use of the substrate solution. The HRP chemiluminescence substrate solution is simple to prepare, the p-Iodophenol serves as the reinforcing agents, the phenolphthalein serves as the synergistic reinforcing agents, the tween-20 serves as stabilizing agents, the stability of the chemiluminescence substrate solution is further improved, and the HRP chemiluminescence substrate solution is stable, high in signal-to-noise ratio and sensitivity, long in platform phase and purple red.

The present invention relates to a process for the preparation of iodinated phenols, —in particular, it relates to a process including the electrochemical iodination of 3,5-disubstituted phenols of formula (1) to the corresponding 3,5-disubstituted-2,4,6-triiodophenols of formula (2), which are useful intermediates for the synthesis of x-ray contrast media, and to the preparation of the contrast media themselves. Furthermore, the present invention includes the electrochemical iodination of 3,5-disubstituted anilines of formula (6) to the corresponding 3,5-disubstituted-2,4,6-triiodoanilins of formula (7).

The invention discloses a preparation method of 4, 4'-biphenol. The preparation method comprises the following steps: adding 4-iodophenol, zinc powder, alkali and a solvent into a reaction bottle, replacing air in the reaction bottle with nitrogen for three times, adding triethylamine formate into the reaction bottle under the protection of nitrogen, stirring for 30-60 minutes, heating to reflux,reacting for 1-16 hours, cooling the reaction liquid to room temperature, filtering, and concentrating filtrate to dryness to obtain 4, 4'-biphenol.

Provided is a method for simultaneously detecting oxytetracycline, tetracycline and kanamycin based on ABEI modified flower-shaped nanogold. The method is characterized by comprising the steps that the chemiluminescence time can be effectively prolonged based on a N-(4-ammonia butyl)-N-ABEI-H2O2-p-iodophenol (PIP) steady-state chemiluminescence system constructed by a microwell plate; a sulfhydrylation complementary chain is connected with ABEI modified flower-shaped nanogold to be used for a signal probe; aptamers fixed on the microwell plate are used as molecular recognition elements, and the aptamers of three antibiotics are fixed on three regions of the microwell plate respectively to provide spatial resolution for simultaneous detection of targets. The constructed aptamer sensor can achieve simultaneous detection on oxytetracycline, tetracycline and kanamycin, and the chemiluminescence intensity of the sensor is in negative logarithm correlation with the quantity of the targets. In addition, the sensor is used for practical sample detection, and the good property of the sensor is proved.

PCT No. PCT / FR96 / 01636 Sec. 371 Date May 9, 1998 Sec. 102(e) Date May 9, 1998 PCT Filed Oct. 18, 1996 PCT Pub. No. WO97 / 15684 PCT Pub. Date May 1, 1997The field of the present invention is that of identification and analysis of chemical and / or biological species of the enzyme / substrate, enzyme / inhibitor or antigen / antibody etc. type. The problem on which the invention is based is to provide a system for qualitative and / or quantitative analysis of biological substances by amplified chemiluminescence which allows an actual significant improvement in the emission of light resulting from passage of a chemiluminescent reagent to the excited state. This problem has been solved by means of a system according to the invention, which involves a ligand a) which can be coupled with the substances to be analysed, a chemiluminescent reagent b) of the luminol type, an enzyme c), a substrate d) which oxidizes the enzyme c), and at least one amplifier e), this system being characterized in that the amplifier e) is chosen from the family of halogenophenol (iodophenol) esters. The invention also relates to an analytical method using this system and to an analytical kit comprising the said system.

The invention discloses a method for preparing an iodophenol compound, comprising the following steps of: dissolving the iodophenol compound in methanol, adding a strong base and potassium iodide with even stirring, dripping a methanol solution of a trichloroiminocyanuric acid, and carrying out separation and purification after the reaction stops completely to obtain the finished product. The molar ratio of the iodophenol compound to the strong base is between 1 to 1 and 1 to 2; the molar ratio of the iodophenol compound to the potassium iodide is between 1 to 1 and 1 to 2; the molar ratio of the iodophenol compound to the trichloroiminocyanuric acid is between 3 to 1 and 3 to 2. The method for preparing the iodophenol compound is nontoxic, free from pollution, low in price and production cost due to the use of the trichloroiminocyanuric acid as an oxidizer. The method also has the advantages of high utilization rate of iodine, good reaction selectivity, easy separation and purification of the finished product, and high yield.

The invention discloses a supermolecular disc liquid-crystal compounds and process for preparation, wherein the compound has the structural formula disclosed in the specification, wherein n=1-6, its preparation process comprises, introducing alkyl into 2-methoxyl-5-iodophenol, carrying out Ullmann coupling, free radical coupling and demethylating reaction, constructing triphenylene precursor, then introducing lateral chain with ester group as the end group, and hydrolyzing the ester group into acids.

To provide a process for producing 2-iodophenol derivatives or 2,6-diiodophenol derivatives by iodinating phenol derivatives at ortho position which can dispense the recovery of iodine and makes it possible to produce the objective iodinated derivatives at low cost, in high yield, and with high quality. High-quality 2-iodophenol derivatives or 2,6-diiodophenol derivatives can be obtained in high yield by reacting a phenol derivative with molecular iodine in the presence of a pyridine and either hydrogen peroxide or iodic acid as oxidizing agent through efficient iodination with a nearly theoretical amount of iodine based on the starting phenol derivative.

The invention relates to the technical field of organic synthesis, in particular to a method for synthesizing 4,4'-biphenol. According to the method for synthesizing 4,4 '-biphenol, provided by the invention, under the catalysis of a nickel catalyst, a coupling reaction of p-iodophenol is promoted by adopting an ultraviolet irradiation condition to prepare 4,4'-biphenol, the method is simple in synthetic route, 4,4'-biphenol can be prepared through one-step reaction, an intermediate does not need to be separated out, the post treatment process is simple and convenient, and the operation is simple. The method has the advantages of mild reaction conditions, easy realization, and low equipment requirements. Meanwhile, a cheap nickel catalyst and copper salts are used together, the cost is low, and the massive production requirements can be better met.

The invention relates to the technical field of drug synthesis, in particular to a preparation method of 2,4,6-triiodoresorcinol. The preparation method of the 2,4,6-triiodoresorcinol comprises the following steps: in a sulfuric acid methanol solution, taking resorcinol as a substrate and taking potassium iodide as an iodo-reagent, dropwise adding a hydrogen peroxide aqueous solution, and reactinghigh-activity new atomic iodine which is generated by oxidizing the potassium iodide through hydrogen peroxide with the resorcinol to produce the product. By-products are water and inorganic salt. The method for synthesizing iodophenol is an oxidizing iodination process, in the process, the generated high-activity iodinating agent-iodine chloride is generated in real time, iodination consumptionis implemented in real time, and the by-products of reaction is water and potassium sulphate; and reaction is green, effective, safe and controllable.

The invention discloses a preparation method of 4,4'-biphenyldiphenol, which comprises: adding 4-iodophenol, zinc powder, alkali and solvent into a reaction bottle, replacing the air in the reaction bottle with nitrogen three times, and protecting the reaction bottle with nitrogen. Add triethylamine formate to the reaction bottle, stir for 30-60 minutes, heat to reflux, react for 1-16 hours, cool the reaction solution to room temperature, filter, and concentrate the filtrate to dryness to obtain 4,4'-biphenyl diphenols.

The invention discloses a supermolecular disc liquid-crystal compounds and process for preparation, wherein the compound has the structural formula disclosed in the specification, wherein n=1-6, its preparation process comprises, introducing alkyl into 2-methoxyl-5-iodophenol, carrying out Ullmann coupling, free radical coupling and demethylating reaction, constructing triphenylene precursor, then introducing lateral chain with ester group as the end group, and hydrolyzing the ester group into acids.

The invention discloses a photodynamic treatment medical composition which consists of a photosensitizer and a chemical activator, wherein the photosensitizer is expressed by the formula I; the chemical activator comprises luminol, p-iodophenol, horse radish peroxidase and hydrogen peroxide; and the proportion of the photosensitizer expressed by the formula I, to the luminol to the p-iodophenol to the horse radish peroxidase to the hydrogen peroxide in the composition is 8-12muM:0.4-0.7mM:1-1.5mM:0.01-0.02mg / mL:2-3mM. In the composition of the invention, active oxygen generated by bioluminescence energy transfer between the photosensitizer expressed by the formula I and the luminol is utilized to kill cells and fungi. Compared with the traditional photodynamic treatment method, the composition can be used for treating in deep tissues; and damages to normal tissues, which are caused by long-time irradiation by an external light source, can be avoided; and meanwhile, the composition has a profound influence on the aspects of the clinic treatment of tumors and pathogenic bacteria infection.

The invention discloses a p-iodophenol synthesis method, which belongs to the technical field of chemical synthesis, and comprises the following steps: adding sodium hydroxide and water into a reaction kettle, then adding phenol and methanol into the reaction kettle, cooling, dropwise adding a methanol solution for dissolving iodine into the reaction kettle, after dropwise adding, carrying out heat preservation and stirring for 2-2.5 hours, sampling and detecting that the phenol is less than 3%, and determining that the product is qualified; then heating to 70 DEG C, completely distilling methanol, adding concentrated hydrochloric acid for acidification, keeping the temperature at 40-45 DEG C for 1-1.5 hours, cooling to 10-20 DEG C, centrifuging to obtain a solid crude product, and leaching with water during centrifuging; adding a solid crude product obtained by centrifugation into petroleum ether for recrystallization, and performing centrifugal drying to obtain a final white solid product; according to the synthesis method disclosed by the invention, the compound is prepared by adopting a phenol direct iodination process route reaction, the process is simplified, the cost is low, and the yield is high.

The invention provides a synthesis process for a fluorine-containing diphenylacerylene liquid crystal of a lactic acid chiral derivative. The method is to synthesize a target compound 1-[(4-alkyloxy-2,3,5,6-tetrafluorophenyl)acetylenyl]phenyl,4-[(1-ethoxcarbonyl)ethyl]terephthalate and comprises the following concrete steps: successively adding 2.00 g (7.56 mmol) of perfluorophenyltrimethylsilylacetylene, 2.00 g (14.44 mmol) of potassium carbonate, 2.60 g (28 mmol) of n-amyl alcohol and 60 mL of DMF into a three-neck flask with a volume of 100 mL and carrying out stirring; (2) adding 5 g of paraphthaloyl chloride into a 40-mL three-neck flask with a solvent dioxane under the protection of nitrogen, carrying out magnetic stirring to dissolve paraphthaloyl chloride, continuing to add 3.6 g of p-iodophenol and 0.1 g of DMAP, then adding a few drops of pyridine drop by drop and carrying out a reflux reaction for 24 h; and (3) adding 560 mg (1.2 mmol) of a compound, 650 mg of Pd (PPh3)2Cl2, 60 mg of CuI and 35 mL of triethylamine into a three-neck flask with a volume of 50 mL under the protection of nitrogen and successively carrying out stirring, a reflux reaction, cooling and pumping filtration.

The invention discloses a reagent kit for detecting ochratoxin A in food. The reagent kit comprises a porous coated plate, a buffer solution, an ochratoxin A standard substance, an ochratoxin A antibody, an europium-marked rabbit anti-mouse antibody, a washing solution and a luminous solution. The luminous solution compris, 2-2.4g / L of polyethylene glycol 600, 0.16-0.18wt% of polysorbate-20, 0.9-1.2g / L of 4-Iodophenylboronic acid, 0.18-0.2g / L of p-Iodophenol, 22-24ml / L of dimethylformamide, 12-14 g / L of polyvinylpyrrolidone, 3-5g / L of ethylenediamine tetraacetic acid, 1-1.2 million units / L of gentamicin sulphate, 0.5-0.55g / L of hydrogen peroxide urea and 0.12-0.15mol / L of tris (hydroxymethyl) aminomethane buffer solution with pH value ranging from 9.2-9.4. The reagent kit for detecting the ochratoxin A in food has the advantages of simplicity, delicacy, accuracy and quickness in operation and is applicable to quick detection for large batches of samples.

The present invention relates to the synthesis process of 2-methoxyl-5-iodophenol. The synthesis process includes the following steps: the reaction of 2-methoxyl-phenol and p-toluenesulfonyl chloride in triethylamine solvent to prepare p-toluenesulfo-(2-methoxyl) phenol ester; the reaction of p-toluenesulfo-(2-methoxyl) phenol ester, metal chloride and iodine monochloride to produce great amount of solid; suction filtering, washing with glacial acetic acid and water washing to obtain p-toluenesulfo-(2-methoxyl-5-iodo) phenol ester; and final deprotection of p-toluenesulfo-(2-methoxyl-5-iodo) phenol ester to obtain 2-methoxyl-5-iodophenol. The present invention has the advantages of simple operation, low material cost, high yield, high product purity, etc. and is suitable for industrial production.

The invention discloses an amphiphilic anticancer photosensitizer with a big two-photon absorption section as well as preparation and application thereof. The preparation of the amphiphilic anticancer photosensitizer comprises the following steps: under the action of ZnP, BD, 4-iodophenol tetrakis (triphenylphosphine) palladium and CuI, preparing an intermediate BD-ZnP-OH; then enabling the intermediate to react with diiodo tetraethylene glycol under the action of potassium carbonate and preparing an intermediate BD-ZnP-I; finally preparing the amphiphilic anticancer photosensitizer BD-ZnP-P through the intermediate and the triphenylphosphine. Through spectroscopic analysis, the obtained very high singlet oxygen quantum yield of the photosensitizer is 49 percent; finally, as the photosensitizer has the very big two-photon absorption section, 1725 GM is reached; meanwhile, in-vitro photodynamic test results in HeLa, A549, MCF-17 and HK-1 tumor cells show that the amphiphilic anticancer photosensitizer has very good tumor cell permeability and tumor cell killing ability under photo-induction.

The present invention relates to the technical field of drug synthesis, in particular to a method for preparing 2,4,6-triiodoresorcinol, that is, in sulfuric acid methanol solution, using resorcinol as a substrate, potassium iodide as an iodine reagent, and dropping Hydrogen peroxide aqueous solution is added, and the highly active, nascent atomic iodine and resorcinol generated by the oxidation of potassium iodide by hydrogen peroxide react to produce products; the by-products are water and inorganic salts. The method for synthesizing iodophenol of the present invention is an oxidation iodization process, and the highly active iodide iodine chloride generated in the process is immediately generated and consumed by iodine immediately, and the by-products of the reaction are water and potassium sulfate; the reaction is green, efficient, and Safe and controllable.

The invention provides a chemiluminescent substrate solution, which comprises the following components: 0.2-1.2g / L of Luminol, 0.1-5g / L of cinnamic acid, 0.05-0.6g / L of 4-iodophenylboronic acid, 0.05-0.6 g / L of p-iodophenol, 5-50ml / L of dimethylformamide (DMF), 2.5-7.5 g / L of polyvinyl alcohol (PVA), 5-15g / L of polyvinyl pyrrolidone (PVP), 1.5-5g / L of polyethylene glycol 600 (PEC -600), 1-10g / L of ethylene diamine tetraacetic acid (EDTA), 400,000-4,000,000 unit / L of gentamicin sulfate, 0.1-1.0g / L of carbamide peroxide and 0.1mol / L of tris buffer solution with a pH vale being 9.0. The chemiluminescent substrate solution is a solution which can be directly used, and maintains wide linear detection range, good stability and high sensitivity, thus solving the problem that a traditional chemiluminescent substrate solution consists of a solution A and a solution B which are stored separately; and after the solution A and the solution B are isovolumetrically mixed for 10 minutes, signal values are decreased sharply, the detection range is narrowed and the stability is poor. In addition, the chemiluminescent substrate solution provided by the invention is easy to prepare, low in cost and simple to operate, environmentally friendly, and non-toxic and harmless to human bodies and the like.

Provided is a method for simultaneously detecting oxytetracycline, tetracycline and kanamycin based on ABEI modified flower-shaped nanogold. The method is characterized by comprising the steps that the chemiluminescence time can be effectively prolonged based on a N-(4-ammonia butyl)-N-ABEI-H2O2-p-iodophenol (PIP) steady-state chemiluminescence system constructed by a microwell plate; a sulfhydrylation complementary chain is connected with ABEI modified flower-shaped nanogold to be used for a signal probe; aptamers fixed on the microwell plate are used as molecular recognition elements, and the aptamers of three antibiotics are fixed on three regions of the microwell plate respectively to provide spatial resolution for simultaneous detection of targets. The constructed aptamer sensor can achieve simultaneous detection on oxytetracycline, tetracycline and kanamycin, and the chemiluminescence intensity of the sensor is in negative logarithm correlation with the quantity of the targets. In addition, the sensor is used for practical sample detection, and the good property of the sensor is proved.

The invention discloses an industrial preparation method of 2-bromo-5-iodophenol. According to the industrial preparation method of the 2-bromo-5-iodophenol, ortho-nitroaniline serves as an initial raw material, and the 2-bromo-5-iodophenol is synthesized by iodination, diazotization bromination, reduction and diazotization phenol hydroxylation four-step reaction. The 2-bromo-5-iodophenol obtainedin the process is blue solid with the purity of 97.5%, raw material conversion rate in each step reaches 100%, and the total yield of the whole process reaches 17%.

The invention provides a preparation method of (R)-(+)-2-(4-hydroxyphenoxy) propionic acid, and belongs to the technical field of pesticide intermediates. Phenol compounds (parachlorophenol, p-bromophenol, p-iodophenol or p-hydroxybenzenesulfonic acid) are adopted as raw materials and react with (S)-(-)-2-chloropropionic acid to synthesize R-(+)-2-(4-chlorophenoxy) propionic acid, and the (R)-(+)-2-(4-hydroxyphenoxy) propionic acid is obtained after hydrogen hydrolysis. The raw material phenol compound used in the invention is low in price and simple in process operation; and the method avoids using hydroquinone as a raw material, and solves the problems of high raw material cost, difficult product purification and low product quality caused by hydroquinone disubstituted impurities generated in the reaction when hydroquinone is used as a raw material for synthesis in the traditional method.

The invention discloses chemiluminescent substrate liquid, which is mainly composed of radiant agent luminol, reinforcing agent p-Iodophenol, oxidant urea peroxide, and is applied to a detection system taking HRP as enzymatic reaction. The chemiluminescent substrate liquid has the characteristics of short reaction time, long detection platform period, good stability and the like, and can enhance the sensitivity of a kit, broaden linear range and prolong the period of validity of the kit.