Quantitative determination kit of chemiluminescence immunoassay for Vitamin B12 (VB 12) and preparation method thereof

Abstract

VB12 The invention discloses a quantitative determination kit of chemiluminescence immunoassay for Vitamin B12 (VB 12), comprising a VB 12 antibody coated plate, an enzyme conjugated VB 12, a VB 12 calibration material, a quality control material, 20 times concentrated washing liquor, luminescent liquors A and B, and sample treatment fluids A and B. additionally, the invention also discloses a method for preparing the VB 12 chemiluminescence immune quantitative determination kit. The advantages of the kit over the prior art are simple operation, and safety and no environmental pollution. Moreover, the kit has the advantages of ability for samples of broad concentration range detection, long effective period, good stability, low cost and the like.

Description

technical field

[0001] The invention relates to the field of immunoanalysis medicine, in particular to a vitamin B12 (VB12) chemiluminescent immunoquantitative detection kit and a preparation method thereof. Background technique

[0002] Vitamin B12 (vitamin B12, VB12), also known as cobalamin, is a cobalt-containing vitamin and a general term for all corrinoids with biological activity of cyanocobalamin. VB12 in nature is synthesized by microorganisms. Plants cannot make VB12, and VB12 is the only vitamin that requires the help of an intestinal secretion (endogenous factor) to be absorbed. In addition, vitamin B12 is also the only vitamin that contains essential minerals. It is red because of cobalt, also known as red vitamin, and it is one of the few colored vitamins. VB12 belongs to group B vitamins, the storage amount is very small, about 2~3mg is in the liver. The human body needs very little vitamin B12, as long as the diet is normal, it will not be deficient. It is...

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