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Gold nano-bar based medicine carrier and preparation technique and use thereof

A technology of gold nanorods and preparation process, which is applied in the field of gold nanorod-based drug carrier and its preparation process and application, which can solve the problems of being easily degraded by nucleases and low transfection efficiency into cells, and achieves strong targeting, The preparation process is simple to operate and the effect of high transfection rate

Inactive Publication Date: 2009-03-11
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Because successful gene therapy mainly depends on how to effectively introduce therapeutic genes with specific therapeutic effects into target cells, and therapeutic genes such as naked antisense oligonucleotides have low transfection efficiency and are easily detected by nucleases. Degradation and other issues
Therefore, how to develop a highly efficient, safe, and non-toxic drug carrier to effectively introduce therapeutic genes such as antisense oligonucleotides into target cells is of great significance for the realization of malignant tumor gene therapy, but so far there is little information about this type of technology The reports of human telomerase catalytic subunit (hTERT) gene are rare, especially the gold nanorod-based drug carrier loaded with hTERT gene antisense oligonucleotides has not been reported.

Method used

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  • Gold nano-bar based medicine carrier and preparation technique and use thereof
  • Gold nano-bar based medicine carrier and preparation technique and use thereof
  • Gold nano-bar based medicine carrier and preparation technique and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Reagents and instruments

[0029] Chlorauric acid (HAuCl 4 ), cetyltrimethylammonium bromide (CTAB), silver nitrate (AgNO 3 ), ascorbic acid (AA) were purchased from Sinopharm Chemical Reagent Co., Ltd., sodium borohydride (NaBH 4 ) was purchased from Shanghai Miura Chemical Co., Ltd., hTERT gene antisense oligonucleotide (5'-GGAGCGCGCGGCATCGCGGG-3') was synthesized by Shanghai Sangong Biotechnology Company, polyethyleneimonium (PEI), sodium polystyrene sulfonate (PSS) was purchased from Aldrich using double distilled water.

[0030] 2. Preparation of gold nanoseeds

[0031] 0.25ml 0.01M HAuCl 4 Add to 9.15ml 0.1M CTAB solution and stir gently, the solution turns orange; add 0.60ml 0.01M NaBH under ice-water bath 4 solution, stirred rapidly for 2 minutes, the solution was light brown; put it in a water bath at 30°C for 2 hours, and set aside.

[0032] 3. Preparation of gold nanorods

[0033] First mix 28.27ml 0.10M CTAB and 0.01M 1.2ml HAuCl 4 Mix gently unde...

Embodiment 2

[0043] 1. Reagents and instruments

[0044] Same as described in Example 1.

[0045] 2. Preparation of gold nanoseeds

[0046] Same as described in Example 1.

[0047] 3. Preparation of gold nanorods

[0048] Same as described in Example 1.

[0049] 4. Preparation of polyanion membrane layer

[0050] First, the aqueous solution of gold nanorods prepared above was centrifuged at 14,000 rpm for 10 minutes, and the supernatant was removed (i.e. discarding the free CTAB in the solution); then the gold nanorods after centrifugation were immersed in a mixed aqueous solution composed of NaCl and PSS In, wherein: the concentration of NaCl is 0.001 mol / l, the concentration of PSS is 100 mg / ml; finally shake the shaker at 35° C. for 10 minutes.

[0051] 5. Preparation of polycation membrane layer

[0052] First the above-mentioned prepared gold nanorod aqueous solution wrapped with PSS film was centrifuged at 14000rpm for 10 minutes, and the supernatant was removed (i.e. discardin...

Embodiment 3

[0056] 1. Reagents and instruments

[0057] Same as described in Example 1.

[0058] 2. Preparation of gold nanoseeds

[0059] Same as described in Example 1.

[0060] 3. Preparation of gold nanorods

[0061] Same as described in Example 1.

[0062] 4. Preparation of polyanion membrane layer

[0063] First, the aqueous solution of gold nanorods prepared above was centrifuged at 5000 rpm for 30 minutes, and the supernatant was removed (i.e. discarding the free CTAB in the solution); then the gold nanorods after centrifugation were immersed in a mixed aqueous solution composed of NaCl and PSS , wherein: the concentration of NaCl is 0.1 mol / l, the concentration of PSS is 1 mg / ml; finally shake the shaker at 4° C. for 60 minutes.

[0064] 5. Preparation of polycation membrane layer

[0065] First, the above-mentioned prepared gold nanorod aqueous solution wrapped with PSS film was centrifuged at 5000rpm for 30 minutes, and the supernatant was removed (i.e. discarding the fre...

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Abstract

The invention discloses a gold nanorods based drug carrier. The carrier is characterized in that: gold nanorods is wrapped with a polyanion film layer, a polycation film layer and an hTERT gene antisense oligonucleotide layer from inside to outside. The gold nanorods based drug carrier is prepared by the layer-by-layer self-assembly principle by utilizing the electrostatic force among radicals, and can be used for preparing inhibitors for malignant tumor cell telomerase. The invention causes hTERT gene antisense oligonucleotide to be loaded on the gold nanorods for the first time to solve the problem of low transfection rate occurred in naked antisense oligonucleotide. The prepared gold nanorods based drug carrier does not only has the advantages of high transfection rate, strong targeting property, obvious effect of inhibiting the activity of the malignant tumor cell telomerase, and the like, but also has the advantages of safety and innocuity, which opens up a new way for the application of the gold nanorods in the biomedicine field; in addition, the invention has the advantages of simple operation for the preparation technology and low cost, and is suitable for scale production.

Description

technical field [0001] The present invention relates to a gold nanorod-based drug carrier and its preparation process and application, specifically, a gold nanorod-based drug carrier loaded with hTERT gene antisense oligonucleotides and its preparation process and application . Background technique [0002] Nano-drug carrier technology uses nanoparticles as a drug-carrying tool, wraps drug molecules in nanoparticles or adsorbs on its surface, and introduces drugs into cells through endocytosis, so as to achieve effective and safe treatment. [0003] Compared with traditional zero-dimensional nanoparticles, the application of gold nanorods in drug carriers has the following advantages: 1) gold nanorods, especially functionalized gold nanorods, have good biocompatibility and low toxicity to biological systems ; 2) Gold nanorods have a high specific surface area, and modifying the biological system on the wall surface can increase the loading capacity; 3) Gold nanorods can be ...

Claims

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Application Information

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IPC IPC(8): A61K47/34A61K47/02A61K48/00A61K31/7088A61P35/00A61K47/32A61K47/36
Inventor 贾能勤陈守慧
Owner SHANGHAI NORMAL UNIVERSITY
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