4 results about "Minicircle" patented technology

Current minicircle production methods are slow, expensive, and difficult to perform under GMP conditions because, in most cases, the product is derived from bacteria. In contrast, HTLA-and CHTLA-based synthetic circular supercoiled DNA production can be done completely in a test tube, using chemically or enzymatically synthesized oligonucleotides, long single stranded DNA, and / or double stranded DNA.

Methods and materials for making and using extracellular vesicles (EVs) engineered to include BMP2 or BMP2 in combination with BMP7, and cells that can be used to generate such EVs, are provided herein. For example, methods and materials for generating EVs that include BMP2 or BMP2 and BMP7, and methods and materials for using such EVs to induce bone formation, are provided. Methods and materials for making and using minicircle DNAs (mcDNAs) to overexpress BMP2 or BMP2 and BMP7 also are provided herein. For example, methods and materials for generating mcDNAs that encode BMP2 or BMP2 and BMP7, and methods and materials for using such mcDNAs to induce bone formation, are provided.

The present disclosure relates to plasmids that may be used to promote prolonged expression of recombinant proteins in vivo, such as in animal models, and their methods of construction and use, for instance in animals. In some embodiments, the plasmids, following injection into an animal, are converted to minicircles by recombination, such as in the liver of the animal.

The present disclosure relates to methods for producing minicircle DNA (mcDNA) and methods of using the mcDNA as a scaffold to produce R-loops. Further disclosed are methods of screening for an agent that binds an R-loop.