41 results about "Minicircle" patented technology

The present invention relates to a plasmid for minicircle production, a method for providing a minicircle and a minicircle produced by said method as well as a pharmaceutical composition comprising the same.

Methods for the production of supercoiled DNA minicircles and their use as substrates are described herein.

The present invention relates to a method for the production of a minicircle. In the method of the present invention, a parent plasmid is provided which has a nucleic sequence flanked by recombination sites. This parent plasmid is exposed to an enzyme which causes recombination at the recombination sites, thereby to form a (i) minicircle comprising the nucleic acid sequence and (ii) a miniplasmid comprising the remainder of the parent plasmid. One recombination site is modified at the 5′ end such that its reaction with the enzyme is less efficient than the wild type site, and the other recombination site is modified at the 3′ end such that its reaction with the enzyme is less efficient than the wild type site, and the other recombination site is modified at the 3′ end such that its reaction with the enzyme is less efficient than the wild type site, both modified sites being located in the minicircle after recombination. This favours the formation of minicircle.

The invention belongs to the optical field and the field of micro-nano systems, and particularly relates to a preparation method of an optical waveguide type minicircle resonant cavity humidity sensorcoated with a TiO2 thin film. The optical waveguide type minicircle resonant cavity humidity sensor includes photoetching on an annular resonant cavity, straight waveguide and input and output grating and coating on the TiO2 thin film on a waveguiding structure. By means of the unique ambulatory mode effect of a minicircle resonant cavity prepared on the SOI waveguide, an optical wave performs resonance in a minicircle, the stable resonance phenomenon can be observed by means of a spectrometer, when the relative humidity of the external world changes, a humidity sensitive thin film absorbs water, the resonant cavity refractive index changes, and then an output spectral line observed on the spectrometer performs wave length shift, and measurement of the humidity is completed. Compared withan ordinary humidity sensor, the optical waveguide type minicircle resonant cavity humidity sensor has the advantages of being easy to operate, low in cost, small in volume, short in reaction time, high in flexibility and not prone to electromagnet interference, and therefore the sensor has the extremely high application value.

The invention relates to a minicircle transfer vector for producing viral vectors comprising a transfer sequence and specific packing signals flanking both sides of the transfer sequence for packaging of the transfer sequence into particles of a viral vector. The invention also relates to minicircle packaging vectors carrying support functions for producing viral vectors. The invention further relates to cells bearing the disclosed minicircles. The invention further relates to methods for producing viral vectors using such minicircles and viral vectors obtained thereby, as well as kits useful in performing the described methods.

The invention belongs to the technical field of bioengineering, and particularly relates to construction and application of a model for researching interaction between HBV cccDNA and a host. The invention provides the construction and the application of the minicircle DNA model highly simulating hepatitis B virus genome cccDNA. By constructing a pmini-MC-HBV-Intron plasmid, a high-purity MC-HBV minicircle plasmid is obtained in vitro; and by optimizing a chimeric intron sequence and a secondary structure of RNA, efficient and variable splicing of the chimeric intron on the RNA level is achieved, and the MC-HBV capable of being copied at a high level in vitro is obtained. The MC-HBV can highly simulate the physiological activity between an HBV virus and host cells. The invention further discloses an in-vitro marker MC-HBV model and a detection technology, and provides the excellent model for revealing the interaction relationship between the HBV cccDNA and the host, and a function regulation mechanism, and exploring a new HBV treatment target.

The invention relates to muscle-targeted minicircle DNA gene therapy, in particular to a minicircle DNA vector for long-term efficient expression of a therapeutic gene product in muscle. The minicircle DNA vector is combined with a muscle targeted delivery technology, and can efficiently express the therapeutic gene product in muscle for a long time, thereby overcoming the defects of limited half-life period of protein drugs and unsustainable efficacy. The minicircle DNA vector can be used for treating common hereditary genetic defects or chronic diseases, and has the advantages of safety, effectiveness and low cost.

The invention relates to MC (Minicircle) DNA (Deoxyribonucleic Acid) for expressing Her2-BTEC [Bridge between Her2 (Human Epidermal Growth Factor Receptor-2)-Positive Cells and Effector Cells] and application thereof, in particular to an MC DNA carrier for expressing an anti-Her2 bispecific antibody in vivo. Her2 positive cancer cells (target cells) are killed by bispecific antibody mediated effector cells (T cells or NK cells). The invention firstly discloses an MC DNA carrier design scheme of MC.Her2-BTEC, and the MC DNA carrier design scheme is suitable for treating cancer related to Her2.

The present invention relates to the production and use of covalently closed circular (ccc) recombinant DNA molecules such as plasmids, cosmids, bacterial artificial chromosomes (BACs), bacteriophages, viral vectors and hybrids thereof, and more particularly to vector modifications that improve expression of said DNA molecules.

The present invention relates to a non-viral minicircle vector expressing a SOX gene, a stem cell into which the vector is introduced, a pharmaceutical composition for preventing or treating a cartilage disease, including the stem cell, and a method for constructing the vector. The transformation of mesenchymal stem cells with MC / SOX-Trio or MC / SOX-Duo, which is a non-viral minicircle vector according to the present invention, can completely exclude the necessity of expensive growth factors that have been indispensably used in inducing the differentiation of mesenchymal stem cells into chondrocytes. Accordingly, the mesenchymal stem cells transformed therewith, when implanted in vivo, can differentiate into chondrocytes by themselves, and thus have an advantage capable of simplifying the existing complicated steps of culturing cells to induce differentiation and then transplanting the cells.Further, unlike existing vector systems in which antibiotic-resistant genes and other bacteria-derived exogenous genes are simultaneously transferred to cells even after transformation, the vector of the present invention minimizes transfer of unnecessary genes into target cells by allowing two or three SOX genes necessary only for differentiation into chondrocytes to be regulated under one promoter, and thus can be utilized as a non-viral vector system in the most advantageous form for use in clinical application of stem cell-gene therapeutic agents.

Compositions and methods are provided for the treatment of an ischemic cardiovascular condition by providing a patient with a novel non-viral minicircle DNA vector comprising polynucleotide sequences that potentiate HIF-1 activity, including RNAi or antisense agents selective for proteins involved in HIF1 inactivation.

The present invention provides minicircle nucleic acid vector formulations for use in administering to a subject, wherein the minicircle nucleic acid vectors include a polynucleotide of interest, a product hybrid sequence of a unidirectional site-specific recombinase, and are devoid of plasmid backbone bacterial DNA sequences. Also provided are methods of producing the subject formulations as well as methods for administering the minicircle nucleic acid vector formulations to a subject. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications.