Turbot muscular cell line establishment method

Abstract

The invention discloses a turbot muscle cell line establishment method. According to the turbot muscle cell line establishment method, turbot muscular tissues are adopted as materials, and primary culture is started by a single cell method; the turbot muscular tissues are cultured in an L15 culture medium with fetal calf serum and fibroblast growth factors, and subculture is performed by a trypsin digestion method; a cryopreservation culture medium with DMSO (dimethylsulfoxide) is adopted for cryopreservation. The turbot muscle cell line establishment method has the advantages that an established turbot muscle cell line has passed by 35 generations and is quick and stable in growth and capable of providing a large quantity of cells for researches on nutrition, immunity, environmental toxicity, gene function analysis and the like.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a method for establishing a turbot muscle cell line. Background technique [0002] Turbot was introduced from the UK by the Yellow Sea Fisheries Research Institute of China in 1992. After the success of large-scale production in 1999, it was promoted and cultivated in the northern coast and became an important object of mariculture. So far, turbot aquaculture has developed into a pillar industry in northern mariculture with an annual output of more than 60,000 tons. [0003] As a carnivorous fish, the dietary protein requirement of turbot is 42%. However, as its high-quality protein source, the annual output of fishmeal is limited, which restricts the further development of the industry. In order to ensure the stable and safe development of China's aquaculture industry, it is urgent to find protein sources to replace fishmeal. The new protein sources involved ...

AI Technical Summary

Problems solved by technology

Over the years, various researches have been carried out around the substitution of protein sources. After the technology integration and processing have realized the partial substitution of fishmeal to a certain extent, they have been stuck in the bottleneck of not being able to further improve the level of fishmeal substitution for a long time.

The fundamental reason is that too much attention has been paid to the physiological and pathological manifestations of fish after protein source substitution, and the deep molecular mechanism has been neglected.