Method for preparing fructooligosaccharide by using baffled bioreactor

Abstract

The invention discloses a method for preparing fructooligosaccharide by using a baffled bioreactor. The method comprises the following steps of: loading biocatalysts including immobilized fructosyl transferase, immobilized cells containing fructosyl transferase and bacteria containing fructosyl transferase into the baffled bioreactor; adding 35 to 60% (w / w) of sucrose solution into a cold / heat jar, heating the sucrose solution up to 35 DEG C to 55 DEG C and controlling pH of the solution to 4.0 to 7.0, pumping the sucrose solution into the baffled bioreactor at a speed of 0.5 to 2.0 m<3> / h through a pump, continuously circulating for 8 to 30 hours, detecting that the content of fructooligosaccharide in the reaction liquid is 55 to 64% by HPLC, and subjecting the reaction liquid to filtration, sterilization, concentration and other refining procedures, to obtain the fructooligosaccharide final product which conforms to the national GB23528-2009 standard. The method reduces energy consumption, adopts the novel biotransformation device, is environment-friendly, has an easily-controllable process, a high yield and a low bacterial contamination rate, and achieves continuous production of fructooligosaccharide.

Description

technical field [0001] The invention relates to a method for biologically preparing fructo-oligosaccharides, in particular to a method for preparing fructo-oligosaccharides using a baffle reactor. Background technique [0002] Fructooligosaccharides (fructooligosaccharides, FOS, hereinafter referred to as FOS), also known as fructooligosaccharides, fructooligosaccharides or kestolitriose oligosaccharides, the molecular formula is G-F-Fn (n = 1, 2, 3, G is glucose, F is fructose), which is composed of sucrose and 1 to 3 fructosyl groups combined with fructosyl groups in sucrose through β-1, 2 glycoside bonds (GF 2 ), Kestose (GF 3 ) and fructopentose (GF 4 ) and other carbohydrates. [0003] Industrial production is produced by microbial fermentation of β-fructosyltransferase (β-fructosyltransferase, EC 2.4.1.99) or β-fructofuranosidase (β-fructofuranoside, EC 3.2.1.26) acting on 35-60% sucrose solution Generated by intramolecular fructoside transfer reaction. At present...

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Problems solved by technology

However, there are the following disadvantages through stirring: 1. Part of the bacteria, immobilized cells, and immobilized enzymes will be broken by mechanical stirring, which will increase the difficulty of separation and purification in the subsequent process; 2. Due to the material and the inner wall of the tank during the stirring process The temperature in the friction tank rises, and cooling equipment should be installed to ensure that the fermentation temperature is within the control range, otherwise the bacteria, immobilized cells, and immobilized enzymes will be inactivated, reducing their transformation ability

3. High equipment investment cost and low production capacity

The disadvantages of using fixed bed equipment to produce fructooligosaccharides are that the resistance of sucrose solution passing through the reaction bed is large, the flow rate is slow, the operating pressure is high, and it is easy to contaminate bacteria, etc.

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