Application of java brucea fruit fat-soluble extract in preparation of drugs for promoting peripheral nerve regeneration

A fat-soluble extract, peripheral nerve technology, applied in the field of biomedicine, can solve problems that have not yet been discovered

Active Publication Date: 2020-08-07
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the research on Bructus javanica is focused on its anti-tumor or dermatological corrosion and wart treatment, and there is no report on the application of Bructus javanica or its main components in diseases related to the nervous system.

Method used

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  • Application of java brucea fruit fat-soluble extract in preparation of drugs for promoting peripheral nerve regeneration
  • Application of java brucea fruit fat-soluble extract in preparation of drugs for promoting peripheral nerve regeneration
  • Application of java brucea fruit fat-soluble extract in preparation of drugs for promoting peripheral nerve regeneration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation of embodiment 1 lipid-soluble extract of Brucea javanica

[0027] Bructus javanica was purchased from Nantong Hospital of Traditional Chinese Medicine, washed with deionized water, dried in an oven at 60-65°C for 2 days, and then ground into powder (300g). Accurately weigh 50 g of Brucea javanica dry powder, put it into an extraction vessel, and soak it in mixed solutions of absolute ethanol and ethyl acetate of different concentrations for 48 hours at room temperature (22°C). Grouping: 95% absolute ethanol + 5% ethyl acetate; 85% absolute ethanol + 15% ethyl acetate; 75% absolute ethanol + 25% ethyl acetate; 65% absolute ethanol + 35% ethyl acetate; 55% absolute ethanol + 45% ethyl acetate; 45% absolute ethanol + 55% ethyl acetate; 35% absolute ethanol + 65% ethyl acetate; 25% absolute ethanol + 75% ethyl acetate; 15% Absolute ethanol + 85% ethyl acetate; 5% absolute ethanol + 95% ethyl acetate. The soaking solutions with different concentrations were...

Embodiment 2

[0028] The extraction effect of the dehydrated alcohol and ethyl acetate mixed solution of embodiment 2 different concentrations

[0029] In order to detect the effect of the fat-soluble mixture of Brucea javanica extracted with different concentrations of absolute ethanol and ethyl acetate on the growth of nerve cells, the experiment took the nerve cell line PC12 cells as the observation model.

[0030] PC12 cells were cultured in DMEM complete medium (containing 10% horse serum, 5% fetal bovine serum, 100 U / ml penicillin, 100 μg / ml streptomycin) in a petri dish, placed in 5% CO 2 , Cultivated in a 37°C incubator. The medium was changed every two days, and the cells were passaged when they grew to 80% confluence. In the experiment, PC12 cells in the logarithmic growth phase were taken, and 5×10 4 / ml Seed cells in 24-well culture plate, 400μl per well. After 24 hours, it was replaced with DMEM medium containing 1% horse serum and 1% fetal bovine serum. The experiments wer...

Embodiment 3

[0034] Example 3 Cytotoxicity Detection of Brucea Brucea Fat-Soluble Extract

[0035] The lipid-soluble extract of Brucea javanica prepared in Example 1 was lyophilized overnight (EYELAFDU-1200, Tokyo), and the obtained powder was put into a 15ml centrifuge tube and stored at 4°C. Prepare a 10μg / mL solution with sterile deionized water, centrifuge at 8000g for 10min, and then prepare the solution to the concentration required for the experiment, and then use a 0.2mm nylon syringe filter (Millipore, USA) to filter and sterilize the cultured PC12 Cytotoxicity tests were performed in cell lines. The CCK8 kit was purchased from Japan Dojin Chemical Research Institute.

[0036] Take PC12 cells in the logarithmic growth phase, digest, count and resuspend, and adjust the cell density to 5×10 5 / mL, inoculate 100 μL per well in a 96-well plate; after the cells adhere to the wall, discard the medium and wash with 0.01 MPBS for 5 min×2 times. The negative control group was 1% horse s...

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Abstract

The invention discloses application of a java brucea fruit fat-soluble extract in preparation of a drug for promoting peripheral nerve regeneration. The java brucea fruit fat-soluble extract is obtained by extracting with a mixed solution of absolute ethyl alcohol and ethyl acetate. The research finds that the java brucea fruit fat-soluble extract can promote the growth of peripheral DRG neuronalneurons and promote the division and proliferation of peripheral nervous system glial cells Schwann cells; and the java brucea fruit fat-soluble extract has the effect of promoting peripheral nerve regeneration.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a fat-soluble extract of Brucea javanica, the components of which can promote the regeneration of peripheral nerves. Background technique [0002] Brucea javanica L.Merr is a small tree or deciduous shrub with a height of about 2m. It is mainly distributed in the tropical and subtropical regions of Hainan, Guangdong, Guangxi and Yunnan along the southern coast of my country. Its dry and mature fruit is Brucea javanica, the shell is gray-black and hard and brittle, and the whole is oblong 6-10mm long and slightly pointed at both ends. After breaking open, there are oval yellow-white seed kernels with a length of 4-7 mm and a diameter of 3-5 mm, which are smooth and oily, odorless, extremely bitter in taste, and cold in nature. Traditional Chinese medicine believes that its functions and indications are: clearing away heat and detoxification, cutting off malaria, stopping dy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P25/02
CPCA61K36/185A61P25/02A61K2236/333Y02A50/30
Inventor 汤欣
Owner NANTONG UNIVERSITY
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