NF2-/- vestibular schwannoma Schwann cell line and establishment method thereof

Abstract

The invention discloses a human NF2- / - vestibular schwannoma Schwann cell line and an establishment method thereof. The establishment method includes: A, separating Schwan cells in tumors to obtain human NF2- / - primary Schwann cells (human NF2- / - Schwann cells), and carrying out primary culture; B, establishing immunodeficient mouse sciatic nerve human NF2- / - primary Schwann cell transplanted tumor model; C, collecting transplanted tumors greater than 1 cm in diameter from mouse sciatic nerves by screening; D, separating Schwann cells, and carrying out primary cell culture; E, carrying out five successive generations and more of passage culture; F, performing screening to obtain NF2- / - vestibular Schwann cells which are consistent to human NF2- / - primary Schwann cells and transplanted tumor primary cell strains in cellular form and main biological characteristic and which are capable of infinite passage. The NF2- / - vestibular schwannoma Schwann cell line herein has the success rate of95% and above to grow transplanted tumors.

Description

Technical field [0001] The present invention relates to the technical field of cell culture, in particular to a NF2 - / - The establishment method of vestibular schwannoma Schwann cell line and its cell line. Background technique [0002] Schwann cells are the main functional cells of the peripheral nervous system. They not only have the functions of migration, adhesion, production of extracellular matrix, formation of myelin sheath, and participation in the repair of peripheral nerves, but also secrete a variety of nerve factors and biologically active substances to promote neurons And the survival of glial cells, inhibit their apoptosis and promote their regeneration, so as to repair the central nerve injury. The tumorous proliferation of Schwann cells will result in the formation of schwannoma (also known as Schwannoma, schwannoma), which is a benign tumor and prolongs peripheral nerve growth. It can occur in all ages and different sexes. When schwannoma compresses nerve tissue...

AI Technical Summary

Problems solved by technology

[0012] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a kind of NF2 - / - The establishment method of the vestibular nerve Schwann cell line and its cells, aiming to solve the problem of NF2 in the prior art - / - Vestibular nerve Schwann cell line cannot be well applied to experimental research

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