Preparation method of bidirectional gradient vascular endothelial growth factor (VEGF) by virtue of nerve conduit

Abstract

The invention discloses a preparation method of a bidirectional gradient vascular endothelial growth factor (VEGF) by virtue of a nerve conduit. The preparation method comprises the following steps: preparing an electrostatic spinning polycaprolactone (PCL) conduit, carrying out bidirectional gradient ammonolysis on the PCL conduit, preparing a bidirectional gradient heparinization conduit by carrying out heparin coupling reaction, and preparing a bidirectional gradient VEGF conduit. According to the invention, a PCL conduit bidirectional ammonolysis device obtains a gradient conduit with high -NH2 density of a central part and the -NH2 density gradually reduced toward two ends; the bidirectional gradient VEGF functionalized nerve conduit provided by the invention is characterized in that quantity of the VEGFs loaded at the central part is high, and the quantity of the VEGFs loaded at the two ends is relatively low; endothelial cells at the two ends of an injured nerve can be enabled to migrate to a high concentration place at the center of the conduit by virtue of chemotaxis, so that directional vascularization of the nerve conduit from the two ends to the central part is accelerated; and a generated blood vessel can serve as a support for guiding Schwann cells to quickly migrate into the nerve conduit, so that regeneration of a nerve is promoted.

Description

technical field [0001] The invention belongs to the technical fields of nerve regeneration tissue engineering and vascular tissue engineering, and in particular relates to a preparation method of a nerve guide with bidirectional gradient vascular endothelial growth factor. Background technique [0002] After nerve injury, Schwann cells are activated, migrate to form Bungner's ribbons, and guide regenerated axons to extend to the damaged distal end and pass through the damaged site. In nerve contusion, due to the integrity of the endoneurium structure, Schwann cells migrate using it as a scaffold. However, the latest research has found that after nerve transection, the junctional structure of the endoneurium is destroyed, and Schwann cells migrate through the regenerated blood vessels as scaffolds. And the directional growth of blood vessels is crucial to guide the directional migration of Schwann cells and the directional growth of axons. [0003] In the case of nerve inju...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing a nerve conduit with a bidirectional gradient of vascular endothelial growth factor, which aims to accelerate the directional vascularization of the nerve conduit by preparing a conduit with a two-way gradient of VEGF, and guide Schwann cells from damaged ends Rapid directional migration, such as in nerve conduits, thereby promoting nerve regeneration and solving the non-directional problem that VEGF conduits promote vascularization without concentration gradients

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