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Artificial articular cartilage based on autologous cells and preparation method thereof

A technology of artificial joints and autologous cells, applied in the field of biological doctors

Active Publication Date: 2009-11-11
MEDPRIN REGENERATIVE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This new type of artificial cartilage overcomes the passive situation that there is no suitable material available in the repair of articular cartilage damage

Method used

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  • Artificial articular cartilage based on autologous cells and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] (1) Prepare electrospinning solution, hydrosol solution containing cytokines and cross-linking agent solution;

[0070] The electrospinning solution is preferably a dichloromethane solution of poly-DL-lactic acid and polycaprolactone with a number average molecular weight of 26000; the weight percentages of the poly-DL-lactic acid and polycaprolactone are 50% and 50% respectively , the total mass percent concentration of the solution is 7%.

[0071] The cross-linking agent solution is 0.1M calcium chloride solution.

[0072] The hydrosol solution containing cytokines adopts alginate solution, and the mass percent concentrations of cytokines TGF-β1, IGF-1, and BMP-2 in the cytokine alginate solution are each 100 ppm.

[0073] Put the prepared 0.1M calcium chloride solution into a cell culture dish with a diameter of 150 mm, and place it on the flat receiver shared by the electrospinning device and the printer. The Hewlett-Packard 550C inkjet printer was refitted accord...

Embodiment 2

[0081] Implementation steps are the same as in Example 1.

[0082] The preferred number-average molecular weight of the electrospinning solution is a dichloromethane solution of poly-DL-lactic acid and polycaprolactone of 26000; the weight percentages of the poly-DL-lactic acid and polycaprolactone are respectively 50% and 50%. 50%, the total mass percent concentration of the solution is 5%.

[0083] The cross-linking agent solution is 100IU / ml thrombin solution;

[0084] The hydrosol solution containing cytokines adopts cytokine fibrinogen solution, and the concentration of BMP-2 is 10 mg / ml.

[0085] The specific operation is to put the configured thrombin solution into a cell culture dish with a diameter of 90 mm, and place it on the flat receiver shared by the electrospinning device and the printer. The Hewlett-Packard 550C inkjet printer was refitted according to the existing patent reports, for example, referring to the method disclosed in US Patent No. 7,051,654, and ...

Embodiment 3

[0089] The preferred number-average molecular weight of the electrospinning solution is a dichloromethane solution of poly-DL-lactic acid and polycaprolactone of 26000; the weight percentages of the poly-DL-lactic acid and polycaprolactone are respectively 50% and 50%. 50%, the total mass percent concentration of the solution is 10%.

[0090] The crosslinking agent solution is selected from 100 mg / ml water-soluble carbodiimide solution;

[0091] The hydrosol solution containing cytokines adopts collagen and polyanion solution of cytokines, wherein the collagen concentration is 1%, polyanion adopts polyglutamic acid with a concentration of 50 mg / ml, and the cytokines include cell directional migration factor SDF-1, The total mass percent concentration of epidermal growth factor, transforming growth factor beta, BMP-2 and dexamethasone is 0.5%.

[0092] The specific operation is to put the prepared carbodiimide solution into a cell culture dish with a diameter of 150mm, and put...

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Abstract

The invention provides artificial articular cartilage based on autologous cells and a preparation method thereof. The artificial articular cartilage comprises a nano bionic support and hydrosol adhered to the nano bionic support, wherein one or more cytokines are coated into the hydrosol. The invention also provides the method for preparing the artificial articular cartilage, which comprises the following steps: preparing an electrostatic spinning solution, a cytokine-containing hydrosol solution and a crosslinking agent solution; using the crosslinking agent solution to receive electrostatic spinning so as to prepare the nano bionic support; and using an ink jet printer to print the cytokine-containing hydrosol solution on the nano bionic support, and curing the hydrosol to obtain the artificial articular cartilage. The three-dimensional support adopted by the invention has ideal degradation speed and is degraded after the regeneration of an articular cartilage layer, can meet the requirement of actual clinical application, can be completely degraded, and has good abrasion resistance and lubricity so as to be capable of replacing the cartilage before the regeneration of the articular cartilage layer.

Description

technical field [0001] The invention relates to an autologous cell-based artificial articular cartilage and a preparation method thereof, belonging to the technical field of biomedicine. Background technique [0002] Articular cartilage is an essential component of joint function. It is composed of cartilage tissue and its surrounding perichondrium. Cartilage tissue is composed of chondrocytes, matrix and fibers. Chondrocytes are the only cells in articular cartilage that constantly secrete new cartilage matrix, eventually forming the cartilage layer. Chondrocytes are located in the cartilage lacunae in the cartilage matrix, and each cell is surrounded by a cartilage capsule, which fills the cartilage lacuna during life and cannot migrate. The hyaline cartilage matrix is ​​very complex and contains a variety of biopolymers, and also contains a variety of appropriate cytokines. These substrates are usually biologically active substances that can affect the shape, metabolism...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/54A61L27/56A61F2/30A61L27/18A61L27/16A61L27/24A61L27/20A61L27/22
Inventor 徐弢袁玉宇
Owner MEDPRIN REGENERATIVE MEDICAL TECH
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