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Tissue mending material with biological activity and preparation method thereof

A technology of bioactive and repairing materials, applied in the field of tissue engineering biomaterials, can solve the problems of immune rejection, foreign body reaction, difficult fusion, etc., and achieve the effect of promoting healing, high biocompatibility, and promoting wound healing

Active Publication Date: 2009-02-18
SHAANXI RUISHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the components of SIS are mainly secreted and synthesized by animal cells, including extracellular matrix components such as collagen, elastin, glycosaminoglycans, fibronectin, and laminin, which are different from those of the human body. It is difficult to integrate with the human body after being in vivo, which may stimulate the body's foreign body reaction or immune rejection, which limits its use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] 1) Acquisition of fibroblasts and epidermal cells: Aseptically obtain human skin tissue, remove the fat layer, cut into a size of 5mm×5mm, digest with neutral protease, separate the epidermis and dermis tissue; digest the dermis with 300U / ml collagenase Tissue, after digestion, the dermal tissue was blown away to collect the cell fluid, and the supernatant was discarded after centrifugation to obtain fibroblasts, which were inoculated into culture bottles to supplement the fibroblast culture medium, expanded and cultured to the required number; the isolated epidermis was treated with 0.25% After the trypsin digestion solution is digested, blow off and collect the cell fluid, centrifuge and discard the supernatant to obtain epidermal cells, inoculate into culture flasks to supplement the epidermal cell culture medium, and amplify and culture to the required number;

[0018] 2), the preparation of decellularized small intestinal submucosa: the porcine jejunum was rinsed wi...

example 2

[0023] 1) Adipose stem cell acquisition: Aseptically obtain human adipose tissue, cut into 1mm 3 Size, after digestion with 400U / ml collagenase solution, the adipose tissue was blown away to collect the cell solution, and the supernatant was discarded after centrifugation to obtain adipose stem cells; after adding the cell culture solution, the cells were suspended by blowing, inoculated into the culture bottle, and expanded to the desired size quantity;

[0024]2) Preparation of decellularized small intestinal submucosa: Rinse sheep jejunum with distilled water, cut into 10cm fragments, soak and sterilize in aqueous solution containing 0.2% peracetic acid and 3% ethanol for 1 hour; rinse with phosphate buffer saline Afterwards, the intestinal segment was dissected longitudinally, and the small intestinal mucosa, muscularis and serosa were mechanically scraped off. After rinsing with PBS, the submucosa was obtained; then, it was placed in 0.8M NaOH solution and treated at 5±2°...

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PUM

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Abstract

The invention relates to a tissue repairing material with bioactivity and a preparation method thereof. The material is made by compounding human body living cells, and extracellular matrixes and cell growth factors synthesized and excreted by the human body living cells on the acellular small intestine submucosa. Natural antigen components are removed from the prepared tissue repairing material with bioactivity, and when the tissue repairing material is applied to a wound, the tissue repairing material can survive and directly take part in the repair of the wound to continuously synthesize and excrete growth factors, guide the cells around the wound to grow in and the creation of blood vessel, and induce the differentiation of stem cells to skin cells, thereby obviously promoting the wound healing; besides, the tissue repairing material not only has part of the characteristics of human body tissues and the good mechanical property of the acellular small intestine submucosa, but also has high biocompatibility to human body, obviously reduces the immune rejection; at the same time, the prepared tissue repairing material can cover the surface of wound, fill the defection of soft tissues, promote the growth and proliferation of the cells around the wound, repair the defection of the soft tissue organs, and promote the wound healing.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering biomaterials, and in particular relates to a bioactive tissue repair material and a preparation method thereof. Background technique [0002] The behavior of cells in organs and tissues not only depends on the genetic sequence inside the cells, but also is largely influenced by external environmental factors, including the interaction between cells and the extracellular matrix. The extracellular matrix not only provides support and protection for cell growth, but more importantly, the interaction between cells and extracellular matrix can regulate the morphogenetic process of cells and affect cell survival, migration, proliferation and functional metabolism. Therefore, it is very important and urgent to prepare extracellular matrix materials that are conducive to seed cell adhesion, proliferation and differentiation in tissue engineering research. [0003] The small intestinal submucos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/38A61L27/26
Inventor 王爱军
Owner SHAANXI RUISHENG BIOTECH
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