47results about How to "Repair defect" patented technology

The invention relates to a method for preparing a biomaterial of acellular small intestinal submucosa, which comprises the steps of preposition treatment, acellular treatment, enzyme treatment, preparations of membranous products and particle products. Compared with the prior art, the biomaterial has higher bioactivity and biocompatibility, no obvious immune rejection and no toxic effect on cells; besides, the biomaterial has a certain mechanical strength and toughness, and variable shape, size and thickness, thereby being convenient for clinical suturing and fixing. At the same time, the preparation method has the advantages of unlimited raw material source, cell-free residues, no ethical issues and being capable of effectively inactivating virus. The prepared products are applicable to the biomedical engineering fields, such as repairing defections of body tissue, serving as tissue filling materials, repairing facial depression deformity, serving as tissue reinforcements to replace fascia, repairing membranous defections and malnourished and infected surfaces of wound, serving as materials for biodegradable stents, and serving as injectable filling materials.

A composition of porous material and gel for preparing artificial bone is prepared from collagen and porous material chosen from alpha-tricalcium phosphate / hydroxy apatite, beta-tricalcium phosphate / hydroxy apatite-coral, calcium phosphate / hydroxy apatite, calcium carbonatel tricalcium phosphate, etc.

The invention provides a nanometer bionic wound-surface cover and a preparation method thereof. The nanometer bionic wound-surface cover comprises a nanometer bionic bracket and hydrosol attached to the bracket, wherein the hydrosol covers one or a plurality of cytokines. The preparation method for the nanometer bionic wound-surface cover provided by the invention comprises the steps of preparingan electrostatic-spinning solution, a cytokine-containing hydrosol solution and a crosslinker solution, preparing the nanometer bionic bracket by use of electrostatic spinning, using an ink-jet printer to print the cytokine-containing hydrosol solution onto the nanometer bionic bracket, and the like, wherein electrostatic spinning and printing can be repeated so as to form the wound-surface covers different in thickness. The preparation method adopts an in-situ autologous stem-cell engineering technique and adopts stem-cell chemotactic factors to attract autologous stem cells to directionally migrate, enter a wound surface and be differentiated according to designed requirements, thereby avoiding inconvenience caused by using viable cells, achieving rehabilitation effects the same with orbetter than that of using the viable cells and having broad application prospects.

The invention relates to a humanized heterogenous extracellular matrix material, which is a dried cellular foam material made by compounding an extracellular matrix and a cell growth factor which are synthesized and excreted by human body cells on a heterogenous extracellular matrix. Cells and natural antigen components are removed from the prepared humanized heterogenous extracellular matrix material, and when the humanized heterogenous extracellular matrix material is applied to the surface of wound, the humanized heterogenous extracellular matrix material can directly take part in the repair of the surface of wound, and the cell growth factor contained in the humanized heterogenous extracellular matrix material can guide the ingrowth of the cells around the wound and creation of blood vessel, and induce the differentiation of stem cells to skin cells, thereby obviously promoting the healing of the surface of wound; besides, the prepared product is a dried product, which greatly prolongs storage life, and not only has part of the characteristics of human body tissues and good mechanical property of the heterogenous extracellular matrix, but also has higher biocompatibility to human body and obviously reduces the immune exclusive reaction; at the same time, the prepared product can cover the surface of wound, fill the defection of soft tissues, promote the growth and proliferation of the cells around the wound, repair the defection of the soft tissue organs, and promote the wound healing.

The invention relates to a damping joint for an external bone fixator and a regulating type bionic external bone fixator. The damping joint for the external bone fixator comprises a damping joint head A, a damping joint head B, a damping joint pin shaft, a gasket groove, a butterfly gasket, a U-shaped fixing clip and a gasket cover, wherein the damping joint head A comprises a spherical damping joint hinge seat and a threaded damping joint connector A arranged on the spherical damping joint hinge seat, the damping joint head B comprises a damping joint hinge plate and a threaded damping joint connector B arranged on the damping joint hinge plate, the damping joint hinge plate is connected with the spherical damping joint hinge seat through the damping joint pin shaft, and the diameter of the damping joint hinge plate is smaller than the diameter of the spherical damping joint hinge seat. The regulating type bionic external bone fixator with the damping joint has the advantages of simple structure, light weight, low cost, regulating capability and flexible operation, and targeted and personalized combination and regulation can be realized.

An injectable pH-sensitive chitosan quaternary ammonium salt hydrogel is prepared through reaction between chitosan and 2.3-epoxypropyl ammonium trimethylchloride, depositing in acetone, washing, baking to obtain chitosan quatemary ammonium salt, dissolving it in acidic solution, dripping the solution of glycerine phosphate while stirring, and heating for gelatinizing.

The invention relates to a tissue repairing material with bioactivity and a preparation method thereof. The material is made by compounding human body living cells, and extracellular matrixes and cell growth factors synthesized and excreted by the human body living cells on the acellular small intestine submucosa. Natural antigen components are removed from the prepared tissue repairing material with bioactivity, and when the tissue repairing material is applied to a wound, the tissue repairing material can survive and directly take part in the repair of the wound to continuously synthesize and excrete growth factors, guide the cells around the wound to grow in and the creation of blood vessel, and induce the differentiation of stem cells to skin cells, thereby obviously promoting the wound healing; besides, the tissue repairing material not only has part of the characteristics of human body tissues and the good mechanical property of the acellular small intestine submucosa, but also has high biocompatibility to human body, obviously reduces the immune rejection; at the same time, the prepared tissue repairing material can cover the surface of wound, fill the defection of soft tissues, promote the growth and proliferation of the cells around the wound, repair the defection of the soft tissue organs, and promote the wound healing.

The invention relates to a culture method and a bioreactor used for coronary curved surface tissue under a combined load, and comprises a tri-gas incubator with O2, CO2 and thermoregulation, conditions for keeping culture fluid fresh, such as perfusion, and active factors added into the culture fluid. A culture blank with coronary curved surface is fixed on a bottom plate of a culture room. In the culture period, the culture is subjected to the action of a rolling load or a sliding load, and / or a twisting and shearing load, and / or an oscillating load so as to culture a culture with coronary curved surface (including flat shape), and the final culture comprises complexes of cell-scaffold, or keeps in vitro tissue. The mechanical condition of combined load and / or the hypoxia environment provided by the invention are close to the mechanical environment of the growth and the development of the tissue, so as to be favorable for the growth and the development of cells and the tissue under the condition of load. The final culture with coronary curved surface can be applied to the restoration of special shape defects.

The invention discloses toothpaste with low abrasion and a high tooth cleaning effect. The toothpaste is prepared from the following components in percentage by weight: 20 to 40 percent of a frictionagent composition, a humectant, a thickener, a surfactant, a sweetener, a preservative, essence, de-ionized water and the like. The friction agent composition is prepared from 5 to 20 percent of cuttlebone and 10 to 30 percent of baking soda (sodium bicarbonate). According to the toothpaste disclosed by the invention, a natural friction agent is prepared by carrying out degreasing and protein removal treatment on the cuttlebone; then the natural friction agent and the baking soda (sodium bicarbonate) form the friction agent composition and the friction agent composition is added into the toothpaste, so that the low-abrasion and high-tooth-cleaning-effect type toothpaste is provided.

A microcrystal glass used for repairing tooth contains K2O (15-25%), Al2O3 (20-30 %), SiO2 (50-60%), CaO (1-6%), MgO (0.1-0.8%), Fe2O3 (0.04-0.1%) and TiO2 (0.04-0.1%), and is prepared through mixingsaid raw materials, grinding, baking, smelting, cold isostatic pressing for shaping, nucleating, crystallizing and heat treating. Its advantages are high strength, and excellent physical and chemicalproperties.

The invention belongs to the field of cartilage tissue repair and tissue engineering and provides a tissue engineered bone cartilage frame and a preparation method thereof. Particularly, marrow removal and degreasing are carried out on allogeneic or xenogeneic cancellous bones to obtain cancellous bone columns with experimentally or clinically required diameters, and then a tissue engineered bonecartilage frame with a completely decalcified upper layer and a superficially decalcified lower layer is obtained via layered decalcification. The upper layer of the frame is used as the framework part of the tissue engineered cartilage frame or an engineering cartilage frame, and the lower layer thereof can be used as a bone forming frame, an engineering bone bracket and the fixed pivot of the engineered cartilage, and can be used for constructing an integrative tissue engineered bone cartilage or directly transplanted for the cartilage or bone cartilage repair. Compared with the existing tissue engineered bone cartilage frame, the invention has the advantages of integration (no interface mechanical defect), and good compatibility of a pore structure and the tissue, has better compression resisting, tensile resisting and shear resisting properties, and is convenient for the minimally invasive surgery operation under an arthroscope.

The invention discloses a bionic periosteum for repairing a segmental bone defect, a periosteum-bone substitute and a preparation method. The bionic periosteum is prepared by culturing osteogenic precursor cells on the surface of polydimethylsiloxane, and removing the cells after forming a cell sheet to obtain an extracellular matrix (ECM) sheet. The bionic periosteum winds the surface of a degradable bone scaffold material to form the periosteum-bone substitute. The prepared osteogenic precursor cell-derived ECM sheet not only has biological activity, but also shows a bone induction effect invitro and in vivo. In addition, the ECM sheet has a chemotactic effect on mesenchymal stem cells. Through combination of the cell-derived ECM sheet and the degradable bone scaffold material, an existing membrane induction technique for repairing the large-area segmental bone defect in clinic can be improved; the preparation method is simple; and the periosteum-bone substitute can be used as an excellent periosteum-bone substitute material.

The invention belongs to a medical biological material technology field, concretes a chitosan fiber film, wherein the fiber film is prepared from a chitosan spinning solution, which is a mixed solution containing chitosan, hexafluroisopropyl alcohol and acetate. The invention further provides a method for preparing the chitosan fiber film, and applications of the fiber film for preparing endocranium or endorhachis defect repairing material, cerebrospinal fluid leakage plugging material or a tissue adhesion prevention medicament.

The invention relates to a preparing method of a dental ceramic composite material. The method includes mixing 15.00-25.00% by mass of K2O, 20.00-30.00% by mass of Al2O3, 48.00-60.00% by mass of SiO2, 1.50-7.00% by mass of CaO, 0.05-1.00% by mass of MgO, 0.01-0.10% by mass of Fe2O3 and 0.01-0.10% by mass of TiO2, adding 75-95% by mass of ethanol, grinding with the granularity of ground powder being controlled to be lower than 4 mu, drying, melting for form glass, smashing and grinding the cooled glass with the granularity of ground powder being controlled to be 4 mu or below, covering microcrystalline alumina ceramic with the ground powder with the thickness being 0.3-3 mm, pressing and performing isostatic cool pressing through a former, nucleating and performing crystallization heat treatment to obtain the dental ceramic composite material. Mechanical performances of the prepared dental ceramic composite material are better than those of leucite-enhanced dental microcrystalline glass at present, and physical and chemical performances of the prepared dental ceramic composite material are better than those of microcrystalline alumina ceramic at present, so that the dental ceramic composite material can be used as restorations for repairing tooth defects. The method is easily available in raw materials and simple in production process and has good development and application prospects.

The invention relates to a preparation method of engineering periodontium, which is used for reconstruction of periodontium. The periodontium comprises periodontal ligament stem cells and an extracellular matrix secreted and synthesized by the periodontal ligament stem cells. The periodontal ligament stem cells are subjected to amplification culture to form multi-layered periodontal ligament stem cell assemblies, and then the periodontal ligament stem cell assemblies are arranged layer by layer and molded to form the engineering periodontium. The periodontium can form a new dentinal structure in the human body, so that periodontal defects can be repaired. The periodontium is simple in preparation, good in plasticity, moderate in cell density, abundant in extracellular matrix content, free of support material, and short in in-vitro culture time.

The invention discloses a preparing method of polypeptide hydrogel. The method includes dissolving polypeptide into a dispersing medium to form a polypeptide solution, and then the polypeptide hydrogel can be formed after several minutes. The polypeptide is formed by n repeated xKEALKEy amino acid sequences, wherein the n is an integer from 2 to 10, the x is a hydrophobic amino acid, the y is a hydrophilic amino acid, the K is lysine, the E is glutamic acid, the A is alanine and the L is leucine. The polypeptide hydrogel and applications thereof are also disclosed by the invention. The preparing method is simple and convenient in operation, components of the polypeptide hydrogel are pure and controllable and the main folding manner of the polypeptide used in the method is an alpha spiral manner, thus avoiding possible safety problems fundamentally. The polypeptide hydrogel prepared by the method can maintain a gel state in a pH range of 5.8-7.6, and is liquid when a pH value is out of the range.

The present invention discloses one kind of cartilage graft, which includes pharmaceutically acceptable biodegradable material and fat stem cell and is in the shape matching the cartilage defect part to be repaired. The tissue engineering cartilage constructed with the homologous fat stem cell may be used in treating cartilage impairment effectively. The present invention also provides the preparation process and use of the tissue engineering cartilage.

The invention relates to a method for preparing a biomaterial of acellular small intestinal submucosa, which comprises the steps of preposition treatment, acellular treatment, enzyme treatment, preparations of membranous products and particle products. Compared with the prior art, the biomaterial has higher bioactivity and biocompatibility, no obvious immune rejection and no toxic effect on cells; besides, the biomaterial has a certain mechanical strength and toughness, and variable shape, size and thickness, thereby being convenient for clinical suturing and fixing. At the same time, the preparation method has the advantages of unlimited raw material source, cell-free residues, no ethical issues and being capable of effectively inactivating virus. The prepared products are applicable to the biomedical engineering fields, such as repairing defections of body tissue, serving as tissue filling materials, repairing facial depression deformity, serving as tissue reinforcements to replace fascia, repairing membranous defections and malnourished and infected surfaces of wound, serving as materials for biodegradable stents, and serving as injectable filling materials.

The invention relates to a canine uterus whole-organ acellular matrix and a preparation method as well as the application of the canine uterus whole-organ acellular matrix. The canine uterus whole-organ acellular matrix is prepared by the following steps: selecting raw materials, pre-treating, removing cells, sterilizing and storing. The canine uterus whole-organ acellular matrix can be used for preparing particles, fluid compositions, gels and active peptides. The canine uterus whole-organ acellular matrix completely reserves the complex three-dimensional array of the natural uterus extracellular matrix and the horizontal-inside longitudinal-outside ultra-structural of the smooth muscle membrane; the canine uterus whole-organ acellular matrix also reserves the complete and non-leaking vascular matrix network as well as reserves a large number of biological active ingredients such as growth factors, hyaluronic acid, amino glucan and laminin, so that the canine uterus whole-organ acellular matrix has high affinity to the endometrial stem cells. Thus, the canine uterus whole-organ acellular matrix has the biomechanical strength and tenacity similar to those of the natural uterus; moreover, the canine uterus whole-organ acellular matrix is thorough in cell removal, free of obvious immune rejection and free of ethical and moral issues.

The invention discloses a preparation method for hydrogel with a stepped hole structure. The preparation method comprises the following steps: (1) separately weighing water-soluble pore-forming agent particles of various dimensions, and sequentially spreading the pore-forming agent into a mould according to the sequence of spreading the small particles first and then spreading the large particles, and compacting the pore-forming agent; (2) dissolving medical polymer into an organic solvent by a heating and stirring method; (3) pouring a polymer solution into a mould containing the pore-forming agent, pressurizing and forming, thereby obtaining a mixture of the solution and the pore-forming agent; (4) putting the mixture of the solution and the pore-forming agent into freezing equipment to freeze for 6-8 hours, unfreezing for 12-16 hours at the room temperature, performing freezing-unfreezing circle once or more, thereby obtaining hydrogel containing the pore-forming agent; and (5) putting the hydrogel into deionized water to stir, changing water every 3-5 hours to remove the pore-forming agent, and finally obtaining the hydrogel with the stepped hole structure. The preparation method is simple and stable in process; and the prepared hydrogel with the stepped hole structure is beneficial for stimulating a layered structure of natural cartilage.

The invention discloses a preparation method of a konjac glucomannan fiber scaffold material, belonging to the field of biomedical materials. The main component of the konjac glucomannan fiber scaffold material is konjac glucomannan, wherein the mass ratio of konjac glucomannan to water is (1:120)-(1:70), the volume ratio of sulfuric acid to water is (1:2,000)-(1:200), the mass percentage of calcium hydroxide in a calcium hydroxide solution is 0.05-2%, and the volume percentage ratio of a calcium hydroxide aqueous solution to absolute ethyl alcohol in crosslinking liquid is (1:2)-(1:0.5). According to the preparation method disclosed by the invention, through sulfuric acid modification and calcium hydroxide aqueous solution crosslinking, calcium sulfate can be generated while chondroitin sulfate is released so that the scaffold material can be better applied to articular cartilage repair.

The invention relates to an application of an insulin-like growth factor binding protein 2 (IGFBP2) to the promotion of mesenchymal stem cell mediated adipose tissue regeneration. The invention furthermore relates to an application of the IGFBP2 to the preparation of drugs for promoting mesenchymal stem cell mediated adipose tissue regeneration, and an application of mesenchymal stem cells for IGFBP2 over-expression to the preparation of drugs for treating adipose tissue regeneration. According to the application, the research discovers that the IGFBP2 has the effect of promoting adipogenic differentiation of the mesenchymal stem cells, so that the adipose tissue regeneration capability of the mesenchymal stem cells can be enhanced by utilizing the IGFBP2, and the IGFBP2 can be predictably applied to the treatment and repair of congenital and acquired adipose tissue defects.

The invention discloses a tissue-engineered alveolar bone tissue and a preparation method thereof. The tissue-engineered alveolar bone tissue comprises a cell polymer containing seed cells and an extracellular matrix and a scaffold material for promoting bone formation through 3D customization. The tissue-engineered alveolar bone tissue can form a new alveolar bone tissue structure in vivo to recover the horizontal or vertical bone height of the alveolar bone. The tissue-engineered alveolar bone tissue has advantages of simple preparation method, shapeability, availability of a variety of seedcells, a large amount of extracellular matrix, and short in vitro culture and preparation period. Compared with other types of alveolar bone technologies existing at present, the tissue-engineered alveolar bone tissue has better biological activity and good biological safety, and can remarkably shorten time for new bone formation.

The invention relates to the field of biology, in particular to a method for preparing corium and corium. The dermis has a long shelf life and can effectively protect the dermal matrix layer and avoid wound infection. The invention overcomes the defects of the prior art that the tissue engineered skin has a short shelf life, high production and storage costs, and easy wound infection during use. The preparation method of the dermis provided by the embodiment of the present invention includes: combining a dermis layer containing living cells with a collagen film and then freeze-drying to obtain a decellularized dermis.

The invention relates to a preparing method of a dental ceramic composite material. The method includes mixing 15.00-25.00% by mass of K2O, 20.00-30.00% by mass of Al2O3, 48.00-60.00% by mass of SiO2, 1.50-7.00% by mass of CaO, 0.05-1.00% by mass of MgO, 0.01-0.10% by mass of Fe2O3 and 0.01-0.10% by mass of TiO2, adding 75-95% by mass of ethanol, grinding with the granularity of ground powder being controlled to be lower than 4 mu, drying, melting for form glass, smashing and grinding the cooled glass with the granularity of ground powder being controlled to be 4 mu or below, covering microcrystalline alumina ceramic with the ground powder with the thickness being 0.3-3 mm, pressing and performing isostatic cool pressing through a former, nucleating and performing crystallization heat treatment to obtain the dental ceramic composite material. Mechanical performances of the prepared dental ceramic composite material are better than those of leucite-enhanced dental microcrystalline glass at present, and physical and chemical performances of the prepared dental ceramic composite material are better than those of microcrystalline alumina ceramic at present, so that the dental ceramic composite material can be used as restorations for repairing tooth defects. The method is easily available in raw materials and simple in production process and has good development and application prospects.

The invention relates to a gum base composition for protecting the balance of oral flora. The gum-based composition is prepared from a gum base, bioactivity mineral powder and polylysine, wherein a weight proportion of the bioactivity mineral powder and the polylysine is (2 to 25) : (0.0003 to 10). According to the gum base composition disclosed by the invention, the bioactivity mineral powder and the polylysine in the gum base composition are capable of inhibiting and killing various pathogenic bacteria in an oral cavity, preventing and treating oral problems of oral infection, oral ulcer, chronic gingisitis and the like, and have long-acting bacteria resistance; meanwhile, the bioactivity mineral powder and the polylysine have a certain selective protection function on beneficial bacteria in the oral cavity, the balance of flora in the oral cavity can be effectively regulated, and normal sanitation and health of the oral cavity can be maintained. Through remineralization of the bioactivity mineral powder on teeth, the gum base composition is capable of effectively repairing dentin defects; the gum base composition provided by the invention is convenient to use, wide in receiver groups and very strong in market application value.

A surgical implant (10) has an areal base structure (12) and at least one projection (14) which is absorbable or partially absorbable and which is designed to take up at least half the implant's own weight of body fluids. In a preferred embodiment, the projection (14) has an attachment area (16) which is sited on the base structure (12) and which is adjoined by a cylindrical or frustoconical middle part (17), pointing away from the base structure (12), followed by a widening (18) arranged at the end remote from the base structure (12).

The invention belongs to a medical biological material technology field, concretes a chitosan fiber film, wherein the fiber film is prepared from a chitosan spinning solution, which is a mixed solution containing chitosan, hexafluroisopropyl alcohol and acetate. The invention further provides a method for preparing the chitosan fiber film, and applications of the fiber film for preparing endocranium or endorhachis defect repairing material, cerebrospinal fluid leakage plugging material or a tissue adhesion prevention medicament.

A multi-channel nerve repair catheter and mold with tissue-inducing function. Chitosan-coated salidroside microspheres and composite salidroside sustained-release microspheres were prepared. The cumulative drug release amount was calculated by different The composite salidroside sustained-release microspheres with high content are mixed with type I collagen to obtain salidroside sustained-release microspheres / type I collagen; the multi-aperture columnar nerve conduit core layer is prepared by using a mold, and the high-voltage electrospinning technology The shell layer of the nanofiber nerve conduit is prepared, and the core layer and the shell layer are nested to obtain a multi-channel nerve repair conduit with tissue-inducing function. The shell of the catheter has a good function of exchanging with interstitial fluid and has the function of guiding nerve growth; the core layer can guide the directional growth of nerve fibers, promote the directional differentiation of stem cells into Schwannsch cells, accelerate the growth of nerve fibers and restore function; effectively repair peripheral Nerve defect, with good degradability and biocompatibility, meets the requirements of tissue engineering scaffold materials.

The invention discloses a preparing method of polypeptide hydrogel. The method includes dissolving polypeptide into a dispersing medium to form a polypeptide solution, and then the polypeptide hydrogel can be formed after several minutes. The polypeptide is formed by n repeated xKEALKEy amino acid sequences, wherein the n is an integer from 2 to 10, the x is a hydrophobic amino acid, the y is a hydrophilic amino acid, the K is lysine, the E is glutamic acid, the A is alanine and the L is leucine. The polypeptide hydrogel and applications thereof are also disclosed by the invention. The preparing method is simple and convenient in operation, components of the polypeptide hydrogel are pure and controllable and the main folding manner of the polypeptide used in the method is an alpha spiral manner, thus avoiding possible safety problems fundamentally. The polypeptide hydrogel prepared by the method can maintain a gel state in a pH range of 5.8-7.6, and is liquid when a pH value is out of the range.