A kind of preparation method of dermis and dermis

Abstract

The invention relates to the field of biology, in particular to a method for preparing corium and corium. The dermis has a long shelf life and can effectively protect the dermal matrix layer and avoid wound infection. The invention overcomes the defects of the prior art that the tissue engineered skin has a short shelf life, high production and storage costs, and easy wound infection during use. The preparation method of the dermis provided by the embodiment of the present invention includes: combining a dermis layer containing living cells with a collagen film and then freeze-drying to obtain a decellularized dermis.

Description

technical field [0001] The invention relates to the field of biology, in particular to the field of tissue engineering, in particular to a method for preparing dermis and the dermis. Background technique [0002] The skin is the largest organ of the human body. It has functions such as barrier, sensation, temperature regulation, secretion and excretion, participation in immune response, and maintenance of internal and external environment stability. With the development of economy, technology and society and the prolongation of the average life expectancy of human beings, there are more and more skin defects caused by various traumas, especially burns and senile diseases. As the gold standard for the treatment of such skin defects, autologous skin transplantation has achieved great results in clinical application. However, autologous skin transplantation needs to cut off the patient's intact skin through surgery, and then cover the patient's skin defect area, which intensif...

AI Technical Summary

Problems solved by technology

For example, a tissue-engineered skin containing two layers of living cells is composed of epidermal living cells and dermal living cells. Although it is very close to human skin, due to the harsh conditions for the growth and preservation of living cells, the tissue-engineered skin The shelf life is short, and the production and storage costs are high, which greatly limits the application of various skin diseases; the other is tissue-engineered skin that only contains the dermis, and the dermis is composed of fibroblasts and scaffolds. For example, it can By seeding fibroblasts on scaffolds and culturing them, fibroblasts proliferate in the scaffolds to form

Although the composition of the tissue-engineered skin is close to the human dermis, it is very easy to cause wound infection when used, which also greatly limits the application of various skin diseases.

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