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A kind of chitosan nanofibrous membrane for dura mater or dura mater repair and its preparation method and application

A technology of chitosan and fiber membranes, applied in spinning solution preparation, fiber chemical characteristics, textiles and papermaking, etc. Carry out clinical promotion and other issues to achieve the effect of easy control of product quality, good application prospects, simple preparation process and product composition

Active Publication Date: 2016-06-15
SHANGHAI HAOHAI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chitosan is a natural alkaline polysaccharide with good biological properties such as biocompatibility, degradability and prevention of tissue adhesion. Although it has been widely used clinically, it is mainly obtained by solution casting The disadvantage of chitosan membrane is that it is brittle, poor in flexibility and stretchability, and cannot be used to repair dura (spinal) membrane defects
This artificial dura mater has the following disadvantages: (1) The structure is complex, requiring the compounding of various cytokines and drugs, and it is also easy to lead to poor biomechanical properties; (2) The preparation steps are cumbersome, and electrospinning and bioprinting technologies need to be stacked layer by layer ; (3) The possibility of industrial transformation is low, and clinical promotion cannot be carried out

Method used

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  • A kind of chitosan nanofibrous membrane for dura mater or dura mater repair and its preparation method and application
  • A kind of chitosan nanofibrous membrane for dura mater or dura mater repair and its preparation method and application
  • A kind of chitosan nanofibrous membrane for dura mater or dura mater repair and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1: Preparation of chitosan dura mater repair material

[0060] (1) Wash the fresh shrimp shells with heads and tails removed, soak them in 5% HCl and 10% NaOH for 12 hours, wash until neutral, dry and grind them, and place the obtained powder at 100°C React in 50% NaOH solution for 24 hours, then wash until neutral, dehydrate with alcohol, and finally vacuum dry to obtain chitosan with a deacetylation degree of 85-95%.

[0061] (2) Dissolve 0.125 g of chitosan in 10 ml of a mixed solution of hexafluoroisopropanol and acetic acid (2% volume fraction of acetic acid), and stir overnight at room temperature to form a stable solution.

[0062] (3) Put the chitosan solution into the syringe, and connect the needle of the syringe to a high-voltage power supply. The solution supply flow was controlled at 5ml / h by a microflow pump, the applied voltage was 15kv, the distance between the high voltage end and the ground end was 15cm, and the rotation speed of the drum u...

Embodiment 2

[0064] Example 2: Research on the Mechanical Properties of Chitosan Nanofiber Membrane Materials

[0065] Cut the chitosan fiber membrane into a sample with a length and width of 40×4mm, and trim the two sides of the sample flat to prevent cracks, then use a spiral micrometer to measure the thickness of each sample, and select different parts for three measurements to get the average value. Then, the tensile mechanical properties were measured by a universal mechanical testing machine, and the rising speed of the crosshead was 2mm / min. The average value of five parallel samples was taken for each test result.

[0066] The tensile strength of the chitosan fiber membrane is 7.93±0.35MPa, and the elastic modulus is 117.9±7.6MPa. figure 2 shown. Much higher than the mechanical properties of nanofiber membranes such as polylactic acid, polycaprolactone and collagen reported in the literature, such as polylactic acid fiber membrane tensile strength is 3.59MPa (ParkWHet.al., Ther...

Embodiment 3

[0067] Example 3: Research on Biocompatibility and Degradability of Chitosan Nanofiber Membrane

[0068] Rabbits were selected as experimental animals, chitosan fiber membranes were embedded in the leg muscles of the rabbits, and the animals were sacrificed 6 weeks after the operation, and the injected muscles were fixed in 10% formalin for gross observation and HE staining Observe and evaluate its biocompatibility.

[0069] The results showed that after 6 weeks, the chitosan nanofiber membrane was completely wrapped by the envelope, and had been significantly degraded, softened into a gel, and had no adhesion with the envelope.

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Abstract

The invention belongs to a medical biological material technology field, concretes a chitosan fiber film, wherein the fiber film is prepared from a chitosan spinning solution, which is a mixed solution containing chitosan, hexafluroisopropyl alcohol and acetate. The invention further provides a method for preparing the chitosan fiber film, and applications of the fiber film for preparing endocranium or endorhachis defect repairing material, cerebrospinal fluid leakage plugging material or a tissue adhesion prevention medicament.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a chitosan fiber membrane, a preparation method thereof, and the use of the fiber membrane in preparing dura (spinal) membrane defect repair materials, cerebrospinal fluid leakage sealing materials or tissue adhesion prevention drugs in the application. Background technique [0002] Cerebrospinal fluid leakage is a clinical complication often encountered in spinal surgery and neurosurgery. Open skull injuries, congenital meningeal defects, tumor metastasis, and other brain diseases caused by traffic accidents, industrial injuries, and wars can all cause hard Leakage of the meninges, leading to cerebrospinal fluid leakage. Mild cases can cause nerve root damage, leading to delayed wound healing; severe cases can cause intracranial infection, arachnoiditis or meningitis, and even develop into a series of life-threatening complications such as brain abscess...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D01F9/00D01D1/02D04H3/00D04H3/16A61L27/20
Inventor 侯春林窦源东郭兴锋魏长征
Owner SHANGHAI HAOHAI BIOLOGICAL TECH
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