The invention discloses a method for extracting exosome from amniotic mesenchymal stem cells and application of the exosome. The exosome is obtained through the steps of amnion separation, washing, epithelial cell removal, shearing, digestion, centrifugation, second-layer suspension taking, resuspension, inoculated culture, subsequent extraction preparation and the like. When the exosome is applied to a medicine for promoting angiogenesis and osteogenesis, vascular endothelial cell aggregation and angiogenesis can be achieved, a microcirculation is reconstructed, blood perfusion is increased,lost of other functions is not caused, and the application prospect is promising. According to the method, the cell extraction process is optimized, the amniotic stem cell extraction efficiency is higher, the purity is higher, and on the premise that more cells are obtained, it can be guaranteed that more exosomes are obtained.