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Application of rumex madaio makino to drugs for treating psoriasis

A psoriasis, therapeutic effect technology, applied in the application field of rhubarb in the treatment of psoriasis drugs, can solve the problems of large side effects of liver and kidney organs, high cost of drugs, and lack of experimental data

Inactive Publication Date: 2018-08-24
新疆维吾尔自治区维吾尔医药研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide the application of rhubarb rhubarb in the treatment of psoriasis drugs, which solves the problem that the drugs for the treatment of psoriasis caused by immune abnormalities in the prior art have great side effects on the liver and kidney organs, and the drugs for the treatment of psoriasis by immunoregulation High cost and lack of experimental data

Method used

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  • Application of rumex madaio makino to drugs for treating psoriasis
  • Application of rumex madaio makino to drugs for treating psoriasis
  • Application of rumex madaio makino to drugs for treating psoriasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Metabolomics study on serum metabolomics of IMQ-induced psoriasis mouse model with 70% ethanol elution fraction of rhizome rhubarb ethanol:

[0030] 1. Preparation of 70% ethanol elution fraction of Rheum rhizome ethanol extract

[0031] Take 500g of rhubarb rhizome ethanol extraction dry paste extracted with 70% ethanol, crush and grind finely, add water to a beaker to form a suspension, and use NaHCO 3 Adjust the pH of the solution to 9-10, stir and sonicate to fully dissolve it, centrifuge, take the supernatant, and adjust the concentration to 0.3g / mL.

[0032] Pour the prepared test sample solution (70% ethanol solution of rhubarb root) on the D101 perforated adsorption resin to start adsorption, the adsorption speed is 2BV / h, after the sample is loaded, use 4BV deionized water to elute to pH is neutral. Then use 70% ethanol-hydrochloric acid (0.10mol / L) solution as the eluent to elute, the eluent volume is 4VB, the elution speed is 1~2BV / h, the eluent is concentr...

Embodiment 2

[0064] Research on the influence of 70% ethanol eluting part of Rumex rhizome on skin histopathological changes of psoriasis mouse model induced by IMQ, the steps include:

[0065] 1. Reagent preparation

[0066] (1) 15% formalin solution: Take 150mL of formaldehyde solution and add 850mL of distilled water to mix evenly, add 4g of sodium dihydrogen phosphate and 6.5g of disodium hydrogen phosphate to the mixture, and dilute to 1000mL;

[0067] (2) 5% trichloroacetic acid solution: take 5g of trichloroacetic acid and add water to 100ml, mix well;

[0068] (3) 1% hydrochloric acid solution: take 2.7mL hydrochloric acid and add water to dilute to 100mL;

[0069] (4) 1% eosin dye solution: take 1g eosin and add distilled water to 100mL, mix well;

[0070] (5) Hematoxylin dye solution: prepare frozen water; take 2g hematoxylin fully dissolved in 400mL distilled water, place the solution on the frozen water; take 4g potassium alum and add it to 400mL distilled water, slightly heat ...

Embodiment 3

[0085] Study on the influence of 70% ethanol eluted part of rhizome rhizome of Rhizoma rhizome on the expression of TGF-β1 and NRP-1 in skin tissue of psoriasis mouse model induced by IMQ, the steps include:

[0086] 1. Paraffin section making

[0087] The preparation process of the paraffin section is consistent with the process of fixation, material collection, dehydration and wax immersion, embedding and sectioning in the skin pathological examination described in Example 2.

[0088] 2. Immunohistochemistry (SP) method

[0089] Reagent preparation: PBS buffer solution: Take 1,000ml (1 bag) and dissolve it in 1,000ml of distilled water to prepare and use, the ratio is 1:1.

[0090] Tris / EDTA Repair Solution: Take 10ml and dissolve it in 500ml of distilled water to prepare it for use, the ratio is 1:50.

[0091] DAB Chromogenic Agent: Dissolve 1 drop of Solution A (about 50μl) in 1,000μl Solution B to prepare it for use, the ratio is 1:200.

[0092] Antibody (TGF-β1): Take...

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Abstract

The invention discloses application of rumex madaio makino to drugs for treating psoriasis, and particularly relates to the rumex madaio makino alcohol extract 70% ethanol eluted part which achieves the treatment effect through a way of immunoregulation. The invention provides pharmacodynamics study of the rumex madaio makino alcohol extract 70% ethanol eluted part on an imiquimod induced psoriasis mouse model and shows that the drug has an improving effect on the skin symptom caused by psoriasis, and meanwhile, can improve the symptoms of combination of psoriasis and liver and renal functiondamage, and thus the drug which is economical, effective, safe and reliable is provided for treatment of psoriasis.

Description

technical field [0001] The invention relates to the field of drugs for treating immunity, in particular to the application of rhubarb in treating psoriasis. Background technique [0002] Psoriasis, commonly known as psoriasis, is a chronic inflammatory skin disease with a long course of disease and a tendency to relapse, and some cases are almost unhealed throughout life. The disease mainly occurs in young and middle-aged people, and has a great impact on the physical health and mental status of patients. The clinical manifestations are mainly erythema and scales, and the disease can occur all over the body. The scalp and the extensor sides of the limbs are more common, and it usually aggravates in winter. Although the etiology of psoriasis has been studied a lot, it is still not very clear so far. It is generally believed to be caused by the following four aspects: heredity, infection, immune abnormality and endocrine factors. Among them, a large number of studies have pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/70A61P17/06A61K125/00
CPCA61K36/70A61K2236/333A61K2236/55A61P17/06
Inventor 热比姑丽·伊斯拉木曼尔丹·尼牙孜阿娜古丽·马合木提玉素甫江·艾力阿不都热衣木·肉孜斯拉甫·艾白
Owner 新疆维吾尔自治区维吾尔医药研究所
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