Skincare stem cell activin and preparation method and application thereof

A technology for beauty and skin care and stem cells, which is applied in the field of stem cell activators and its preparation for beauty and skin care. It can solve the problems of cumbersome methods, high cost, and low concentration of growth factors, and achieve the goal of maintaining skin elasticity, healing skin damage, and avoiding the risk of cancer. Effect

Inactive Publication Date: 2019-03-19
深圳中旭细胞再生医学研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the existing methods for preparing stem cell growth factors are cumbersome, costly, and the concentration of growth factors obtained is low, so it is urgent to invent a new method for preparing stem cell growth factors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] This embodiment discloses a preparation method of stem cell activator for beauty and skin care, which specifically includes the following steps:

[0036] The freshly collected umbilical cord tissue was washed twice with PBS on a 100cm dish, and then cut into 3cm small sections with sterile scissors; the small section was cut longitudinally, 2 arteries and 1 vein were removed, and washed with PBS; with sterile scissors Cut the tissue into pieces, the more broken the better, add DMEM at a ratio of 1:3, add 0.05% trypsin, 200U / ml (or 0.2mg / ml) collagenase IV. Place in a 37-degree incubator, digest for 3-5 hours; then add 25pg / ml hyaluronidase, 37°C, 5-10min; then filter out impurities with a 200-mesh sterile filter, and filter out the cells centrifuge at 1000rpm for 5-10min, discard the supernatant; add FBS or DMEM to wash, then add DMEM containing 10% FBS to suspend MSCs cells, and repeatedly pipette to make cell suspension. Then add it to a 75cm cell culture flask accor...

Embodiment 2

[0040] The stem cell activator obtained in Example 1 was used to detect the contents of EGF (epidermal growth factor), FGF (fibroblast growth factor) and VEGF (vascular endothelial growth factor) using an ELISA kit. Using a commercial detection kit and detecting with a microplate reader, we found that the content of EGF was 0.5 ng / ml, the content of FGF was 0.1 ng / ml, and the content of VEGF was 1 ng / ml.

Embodiment 3

[0042] This embodiment discloses a kind of preparation method of essence, comprising the following steps:

[0043] (1) Configuration of essence matrix: 0.1% hyaluronic acid, 0.2% allantoin, 0.1% hydroxyethyl cellulose, 0.1% dipotassium glycyrrhizinate, according to the volume of 20ml, add in proportion and add deionized water to set Make up to 18ml, stir to dissolve;

[0044] (2) Add 2ml of the stem cell activator obtained in Example 1 to the matrix obtained in step (1), stir evenly and filter to sterilize.

[0045] The essence has the functions of beautifying, anti-aging, repairing cell damage, enhancing skin elasticity, and making the skin softer and more elastic.

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PUM

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Abstract

The invention belongs to the field of biology, and particularly relates to a skincare stem cell activin and a preparation method and application thereof. The preparation method of the skincare stem cell activin includes the steps of S1, culturing stem cells in vitro; S2, collecting the stem cells, performing cell lysis to obtain cell lysate, subjecting the cell lysate to low-speed centrifuging, removing cell debris, and freezing to preserve supernate to obtain the stem cell activin. Compared with general chemical skincare products, the skincare stem-cell activin has the advantages that under action of the cell signal regulation mechanism and the synergistic effect of various growth factors, nutrition metabolism of epithelial cells can be improved, skin protection can be achieved, skin injury caused by various reasons can be prevented and cured, skin collagenocyte growth can be accelerated, collagen secretion of skin can be accelerated, and further, wrinkle resistance and ageing resistance can be achieved. Therefore, the skincare stem cell activin has good application prospect in fields of medicines, healthcare and beautifying.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a stem cell activator for beauty and skin care, a preparation method and application thereof. Background technique [0002] As the outermost tissue of the human body, the skin plays a role in protecting the human body from external stimuli or injuries. Skin care is considered the most important chapter in cosmetology as the skin ages significantly in terms of visual appearance, physical properties and physiological functions. Skin problems are often clinically manifested as roughness, dryness, increased sensitivity and fragility, decreased elasticity, and increased wrinkles. These are all caused by aging and slowing metabolism. [0003] Stem cells are a type of pluripotent cells with self-replicating ability, known as the "source of immortality", and have great application prospects in the field of regenerative medicine. Stem cells can be divided into embryonic stem cells an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/64A61Q19/00A61Q19/08A61P17/02C12N5/0775
CPCA61K8/64A61K8/99A61K2800/5922A61Q19/00A61Q19/08A61P17/02C12N5/0668C12N2509/00
Inventor 吴中泽卓成柳邓炎滨许家森
Owner 深圳中旭细胞再生医学研究有限公司
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