Method for extracting exosome from amniotic mesenchymal stem cells and application of exosome

Abstract

The invention discloses a method for extracting exosome from amniotic mesenchymal stem cells and application of the exosome. The exosome is obtained through the steps of amnion separation, washing, epithelial cell removal, shearing, digestion, centrifugation, second-layer suspension taking, resuspension, inoculated culture, subsequent extraction preparation and the like. When the exosome is applied to a medicine for promoting angiogenesis and osteogenesis, vascular endothelial cell aggregation and angiogenesis can be achieved, a microcirculation is reconstructed, blood perfusion is increased,lost of other functions is not caused, and the application prospect is promising. According to the method, the cell extraction process is optimized, the amniotic stem cell extraction efficiency is higher, the purity is higher, and on the premise that more cells are obtained, it can be guaranteed that more exosomes are obtained.

Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for extracting exosomes derived from amniotic mesenchymal stem cells and an application thereof. Background technique

[0002] Repair of bone defects caused by trauma, infection, or tumor resection remains a major challenge in jaw defects. The use of autologous bone grafts, most commonly from the iliac crest, is considered the gold standard. Drawbacks are donor site morbidity and graft size limitations. The process of harvesting an autograft from healthy bone increases surgical time and may be associated with potential blood loss and infection risks. In addition, patient age and metabolic disturbances may also affect the quality of autografts. Inconsistent or low concentrations of endogenous mesenchymal stem cells (MSCs) can significantly reduce the efficacy of autologous transplantation. Therefore, bone tissue engineering methods that can help ove...

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