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Preparation method for hydrogel with stepped hole structure

A technology of stepped holes and structured water, applied in medical science, tissue regeneration, prostheses, etc., can solve problems such as single pore size and pore structure, single composition and structure, mechanics, and biology that cannot meet the needs of articular cartilage repair, etc., to achieve The process is stable, the process is simple and the effect

Inactive Publication Date: 2017-05-31
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional tissue engineering scaffold materials usually have a single pore size and pore structure. After seed cell culture, the composition and structure of the tissue obtained are single, which is inconsistent with the layered structure of natural cartilage. Usually can not meet the repair needs of articular cartilage in terms of performance

Method used

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  • Preparation method for hydrogel with stepped hole structure
  • Preparation method for hydrogel with stepped hole structure

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The preparation method of the stepped pore structure hydrogel of the present embodiment:

[0027] (1) The mold used in this embodiment is as figure 1 As shown, there is an exhaust port 2 at the bottom of the mold 1, and each NaCl particle 3 with a size range of 50-100 μm, 200-300 μm, and 400-500 μm is weighed 0.1 g each, and the NaCl particles are weighed in the order of small particles first and then large particles. 3. Spread on the mold one by one and compact it; the porogen in the mold is spread in layers according to the particle size, and the holes at the bottom of the mold are conducive to the discharge of air during the molding process.

[0028] (2) Weigh 6g of PVA and pour it into 34mL of DMSO solvent, stir at 110°C for 3 hours to dissolve it completely, then pour the solution into the mold, and shape it under a pressure of 10MPa until there is solution in the hole at the bottom of the mold Release pressure when flowing out;

[0029] (3) Place the mold in a f...

Embodiment 2

[0032] The preparation method of the stepped pore structure hydrogel of the present embodiment:

[0033] (1) Weigh 1 g of NaCl particles with sizes ranging from 1 to 50 μm, 300 to 400 μm, and 500 to 800 μm, respectively, spread the NaCl particles on the mold in the order of small particles first and then large particles and compact them;

[0034] (2) Weigh 6g of PVA and pour it into 30mL of DMSO solvent, stir at 110°C for 2 hours to dissolve it completely, then pour the solution into the mold, and shape it under a pressure of 5MPa until there is solution in the hole at the bottom of the mold Release pressure when flowing out;

[0035] (3) Place the mold in a freezer for 8 hours, then thaw it at room temperature for 16 hours, and cycle 9 times like this to make cross-linking between PVA molecules;

[0036] (4) Take the hydrogel out of the mold and put it into deionized water for stirring, and change the water every 2 hours until the NaCl particles are fully dissolved to obtain...

Embodiment 3

[0039] The preparation method of the stepped pore structure hydrogel of the present embodiment:

[0040] (1) Weigh 1 g of sucrose granules with sizes ranging from 1 to 50 μm, 300 to 400 μm, and 500 to 800 μm respectively, spread the sucrose granules on the mold and compact them sequentially in the order of small granules first and then large granules;

[0041] (2) Weigh 6g of PLGA and pour it into 40mL of DMSO solvent, stir at 110°C for 3 hours to dissolve it completely, then pour the solution into the mold, and shape it under a pressure of 20MPa until there is solution in the hole at the bottom of the mold Release pressure when flowing out;

[0042] (3) Place the mold in a freezer for 8 hours, then thaw it at room temperature for 16 hours, and cycle 10 times like this to make cross-linking between PLGA molecules;

[0043] (4) The hydrogel was taken out from the mold and put into deionized water for stirring, and the water was changed every 2 hours until the sucrose particles...

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Abstract

The invention discloses a preparation method for hydrogel with a stepped hole structure. The preparation method comprises the following steps: (1) separately weighing water-soluble pore-forming agent particles of various dimensions, and sequentially spreading the pore-forming agent into a mould according to the sequence of spreading the small particles first and then spreading the large particles, and compacting the pore-forming agent; (2) dissolving medical polymer into an organic solvent by a heating and stirring method; (3) pouring a polymer solution into a mould containing the pore-forming agent, pressurizing and forming, thereby obtaining a mixture of the solution and the pore-forming agent; (4) putting the mixture of the solution and the pore-forming agent into freezing equipment to freeze for 6-8 hours, unfreezing for 12-16 hours at the room temperature, performing freezing-unfreezing circle once or more, thereby obtaining hydrogel containing the pore-forming agent; and (5) putting the hydrogel into deionized water to stir, changing water every 3-5 hours to remove the pore-forming agent, and finally obtaining the hydrogel with the stepped hole structure. The preparation method is simple and stable in process; and the prepared hydrogel with the stepped hole structure is beneficial for stimulating a layered structure of natural cartilage.

Description

technical field [0001] The invention relates to a method for preparing a porous hydrogel, in particular to a method for preparing a hydrogel with a stepped pore structure. Background technique [0002] Articular cartilage disease is one of the main diseases affecting public health. There are nearly 40 million joint disease patients in my country. The emergence of tissue engineering technology provides a new method for the repair of articular cartilage defects. Through tissue engineering technology, the regeneration and repair of articular cartilage defects become possible. In tissue engineering, the material selection of the seed cell scaffold is particularly important. Tissue engineering scaffold materials can not only affect the biological properties and culture efficiency of cells, but also determine whether the scaffold can be well combined with the receptor to perform its repair function after transplantation. Scaffold materials usually have a through-hole pore struct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/16A61L27/52A61L27/56
CPCA61L27/18A61L27/16A61L27/52A61L27/56A61L2430/06C08L29/04C08L67/04
Inventor 任力王迎军康军沛杨军忠刘卅
Owner SOUTH CHINA UNIV OF TECH
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