A kind of polypeptide hydrogel, its preparation method and application
A technology of hydrogel and polypeptide solution, which is applied in the direction of prosthesis, aerosol delivery, and non-effective components of polymer compounds, etc., which can solve the problems affecting the application and achieve high safety, easy control of mechanical strength, and simple operation Effect
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Embodiment 1
[0039] First synthesize a biologically active high-purity polypeptide (powder, its amino acid sequence is xKEALKEyxKEALKEyxKEALKEY, K is lysine, E is glutamic acid, A is alanine, L is leucine, x is glutamic acid, y is leucine).
[0040] The polypeptide can be synthesized by any one of the following two methods:
[0041] i Synthesize by chemical method, that is, use a peptide synthesizer to synthesize a specific sequence of peptides, which can be used after purification;
[0042] ii Through biological production, that is, introducing related genes into Escherichia coli or CHO cells to produce polypeptides with specific sequences, isolating high-yielding cell lines, conducting expanded culture, and obtaining polypeptides after purification.
[0043] The synthesized polypeptide was made into a solution with a concentration of 3mg / mL with deionized water, and then a small amount of alpha-MEM medium solution was added dropwise. After standing for several minutes at 37°C and pH 7, ...
Embodiment 2
[0046] First synthesize a biologically active high-purity polypeptide (powder, its amino acid sequence is xKEALKEYyxKEALKEYyxKEALKEYy, K is lysine, E is glutamic acid, A is alanine, L is leucine, x is histidine , y is valine). Wherein, the synthesis method of the polypeptide is the same as that in Example 1.
[0047] The polypeptide is made into a solution with a concentration of 0.5mg / mL with deionized water, and the mixed solution is injected into the irregularly shaped tissue defect area with a sterile syringe, and in situ self-assembles to form a hydrogel under the induction of interstitial fluid (such as blood) , as a scaffold material to promote the repair of tissue defects.
Embodiment 3
[0049] First synthesize a biologically active high-purity polypeptide (powder, its amino acid sequence is xKEALKEYyxKEALKEYyxKEALKEYy, K is lysine, E is glutamic acid, A is alanine, L is leucine, x is glutamine , y is tyrosine). Wherein, the synthesis method of the polypeptide is the same as that in Example 1.
[0050] Make the polypeptide material into a solution with a concentration of 10mg / mL with deionized water, and then add a certain amount of FITC fluorescently labeled EGF dropwise to make the final concentration 1-10mg / mL, at 37°C and pH 7 After standing for a few minutes, the polypeptide hydrogel gradually formed. image 3 The release situation of 1mg / mLEGF and 10mg / mLEGF in the polypeptide hydrogel is shown (wherein, the curve located at the top shows the release situation of 10mg / mLEGF in the polypeptide hydrogel, and the curve located at the bottom shows the release situation of 1mg / mLEGF The release of mLEGF in the polypeptide hydrogel); Figure 4 On the 21st d...
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