Canine uterus whole-organ acellular matrix and preparation method as well as application of canine uterus whole-organ acellular matrix

Abstract

The invention relates to a canine uterus whole-organ acellular matrix and a preparation method as well as the application of the canine uterus whole-organ acellular matrix. The canine uterus whole-organ acellular matrix is prepared by the following steps: selecting raw materials, pre-treating, removing cells, sterilizing and storing. The canine uterus whole-organ acellular matrix can be used for preparing particles, fluid compositions, gels and active peptides. The canine uterus whole-organ acellular matrix completely reserves the complex three-dimensional array of the natural uterus extracellular matrix and the horizontal-inside longitudinal-outside ultra-structural of the smooth muscle membrane; the canine uterus whole-organ acellular matrix also reserves the complete and non-leaking vascular matrix network as well as reserves a large number of biological active ingredients such as growth factors, hyaluronic acid, amino glucan and laminin, so that the canine uterus whole-organ acellular matrix has high affinity to the endometrial stem cells. Thus, the canine uterus whole-organ acellular matrix has the biomechanical strength and tenacity similar to those of the natural uterus; moreover, the canine uterus whole-organ acellular matrix is thorough in cell removal, free of obvious immune rejection and free of ethical and moral issues.

Description

technical field [0001] The invention belongs to the field of whole organ acellular matrix, in particular to a canine uterine whole organ acellular matrix and its preparation method and application. Background technique [0002] Uterine factor infertility (UFI) caused by severe endometrial repair disorder or uterine absence is still a clinical problem. Existing treatment options include surrogacy and uterus transplantation. Surrogacy faces many social and ethical issues and is banned in most countries. Although uterus transplantation has been successfully applied in clinical practice and restored pregnancy function, there are still insufficient sources of donor uterus. , Difficult operation, poor vascular anastomosis, difficult reconstruction of uterine support structure and immune rejection. A current research trend in UFI treatment is to use tissue engineering and regenerative medicine technologies to achieve partial or full-thickness and functional reconstruction of the u...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a canine uterine whole organ acellular matrix and its preparation method and application. The canine uterine whole organ acellular matrix completely retains the complex three-dimensional arrangement of the natural uterine extracellular matrix. The ultrastructure of the longitudinal smooth muscle membrane retains a complete and non-leakage vascular matrix network, retains a large number of bioactive components such as growth factors, hyaluronic acid, aminodextran, and laminin, and is highly compatible with endometrial stem cells , with biomechanical strength and toughness similar to the natural uterus, complete decellularization, no obvious immune rejection, no ethical and moral issues

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