Nano bionic wound-surface cover and preparation method thereof

Abstract

The invention provides a nanometer bionic wound-surface cover and a preparation method thereof. The nanometer bionic wound-surface cover comprises a nanometer bionic bracket and hydrosol attached to the bracket, wherein the hydrosol covers one or a plurality of cytokines. The preparation method for the nanometer bionic wound-surface cover provided by the invention comprises the steps of preparingan electrostatic-spinning solution, a cytokine-containing hydrosol solution and a crosslinker solution, preparing the nanometer bionic bracket by use of electrostatic spinning, using an ink-jet printer to print the cytokine-containing hydrosol solution onto the nanometer bionic bracket, and the like, wherein electrostatic spinning and printing can be repeated so as to form the wound-surface covers different in thickness. The preparation method adopts an in-situ autologous stem-cell engineering technique and adopts stem-cell chemotactic factors to attract autologous stem cells to directionally migrate, enter a wound surface and be differentiated according to designed requirements, thereby avoiding inconvenience caused by using viable cells, achieving rehabilitation effects the same with orbetter than that of using the viable cells and having broad application prospects.

Description

technical field [0001] The invention relates to a nano bionic wound covering and a preparation method thereof, belonging to the technical field of biomedicine. Background technique [0002] In the life activities of the human body, skin defects are often caused by different reasons such as mechanical trauma, physical, chemical and biological damage. Skin injuries that only reach the epidermis or superficial dermis or a small area of ​​deep dermis can be healed by bandaging and applying surgical auxiliary wound coverings, relying on the original skin's own regeneration ability. The role of these surgical accessories wound covering products is to temporarily cover burn wounds, prevent infection and loss of water and body fluids, and may accelerate wound healing. However, the skin damage that injures a large area of ​​deep dermis or the complete loss of the epidermis cannot rely on the original skin's own regeneration ability to recover, and skin transplantation must be perfor...

AI Technical Summary

Problems solved by technology

Allogeneic skin derived from human cadavers has the disadvantage of lacking living cell components and limited sources. Allogeneic epidermal cells contained in artificial skin can cause immune rejection, which cannot be controlled by current general immunosuppressants and cannot be completely eliminated. Immunogenic, risk of viral transmission

Even with highly effective immunosuppressants, it is difficult to ensure that the skin graft will not be rejected

The disadvantages of synthetic materials are: no skin cell components, and the structure is quite different from the natural skin, and it also lacks suitable pore size and rationally distributed growth factors for release control, and cannot effectively induce skin stem cells to grow in, nor can it induce its rational growth. differentiation

[0013] ① The effect is average, it cannot achieve the effect of complete repair of the dermis, does not contain living cells, cannot regenerate the skin, and can only be repaired by the original skin;

[0014] ②It is not suitable for severe injuries, usually only effective for injuries to the epidermis and superficial dermis or a small area of ​​deep dermis damage, and the effect on chronic wounds is very limited; for third-degree burns and large-area deep second-degree burns and skin injuries of the same degree Ineffective, cannot replace artificial skin

[0015] ③This type of dressing has its own scope of application and different disadvantages

[0017] ①Due to the lack of living cell components, the skin cannot be regenerated, and the original skin can only be repaired, so the regeneration and proliferation ability of skin cells and its role in skin repair cannot be fully exerted, so that the repair effect achieved is limited

[0018] ②The structure of most artificial materials is quite different from that of natural skin, lacking suitable pore size and rationally distributed growth factors for release control, which cannot effectively induce the growth of skin stem cells, nor can it induce their rational differentiation

Limited effectiveness of wound repair

[0019] ③In the clinical research of skin transplantation, it is also found that the mechanical properties of artificial materials are poor, there is no suitable pore size after transplantation, and it is not conducive to the growth of surrounding blood vessels, and it is not as elastic and soft as autografted skin

[0020] ④At present, natural materials that do not contain living cell components are mostly derived from animal or allogeneic dermis. The main component is collagen, so the immunogenicity cannot be completely eliminated. The xenogeneic / allogeneic epidermal cells contained in xenogeneic / allogeneic artificial skin can cause immunity. Rejection, which cannot be controlled by current general immunosuppressants, cannot completely eliminate immunogenicity. Even with high-efficiency immunosuppressants, it is difficult to ensure that skin grafts will not be rejected

[0021] ⑤The use of heterogeneous / alternative dermis cannot completely avoid the risk of carrying viruses and spreading diseases

[0023] ⑦The cost of natural materials is high, the modification is complicated, and the source is limited

[0025] ①The current in vitro culture process has a long period, and it is difficult to meet the clinical needs in time

[0026] ②During skin transplantation, it was also found that the tissue-engineered artificial skin has no vascular system to supply nutrients, so the vascularization is slow, and the surface epithelium is prone to necrosis and peeling off.

[0027] ③Because the transplanted epidermal membrane is thin and brittle, it cannot be firmly adhered to the basement membrane and lacks normal functional stability. Therefore, after transplantation, it will inevitably lead to weakening of many functions and appearances, such as scar shrinkage and vesicle formation. There is still a big gap between the toughness and mechanical properties of skin and natural skin

[0028] ④Most artificial skin uses allogeneic epidermal cells, which can cause immune rejection, which cannot be controlled by current general immunosuppressants, and the skin will eventually necrotic and fall off. Currently, it cannot be used for large-area deep burn wounds

[0029] ⑤ Allogeneic and heterogeneous artificial skin not only cannot completely eliminate immunogenicity, but may also carry viruses and spread diseases

However, bioprinting technology is still in the stage of basic research, and there have not been any artificial organs prepared directly from cells through bioprinting technology, and no relevant reports have been seen.

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