Anti-bioadhesion polyelectrolyte gel as well as preparation method and application thereof

A polyelectrolyte and hydrogel technology, applied in the field of biomedical materials, can solve the problems of weak electrostatic effect, inability to stably attach, hinder the adhesion of biological macromolecules, etc. Effect

Active Publication Date: 2018-03-06
TIANJIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its antifouling mechanism mainly has the following two points: (1) Zwitterions achieve high hydration through electrostatic interaction, so that the hydration layer formed on the molecular surface greatly hinders the adhesion of biological macromolecules (such as proteins) and bacteria and cells; (2) Since zwitterionic molecules have both a positive charge and a negative charge on the same monomer unit, they are apparently electrically neutral, while biomacromolecules, bacteria, and cells often carry positive or negative charges, Therefore, the electrostatic interaction between them is extremely weak, resulting in the unstable attachment of these charged substances to the surface of the hydrogel.

Method used

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  • Anti-bioadhesion polyelectrolyte gel as well as preparation method and application thereof
  • Anti-bioadhesion polyelectrolyte gel as well as preparation method and application thereof
  • Anti-bioadhesion polyelectrolyte gel as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0057] Embodiment 1: the preparation of sodium alginate-polyethyleneimine hydrogel

[0058] Dissolve 0.5 g of sodium alginate powder (molecular weight 300 kDa) in 50 mL of ultrapure water and stir overnight to obtain a 1% sodium alginate solution as a polyanionic polymer solution with a measured charge density of 0.68 mmol / g. 1 g of branched polyethyleneimine (molecular weight 70 kDa) was dissolved in 100 mL of ultrapure water to prepare a polyethyleneimine solution with a mass fraction of 1% as a polycationic polymer solution, and the measured charge density was 14.96 mmol / g.

[0059] Take 1mL sodium alginate solution and add it into a small beaker, then add 0.045mL polyethyleneimine solution dropwise into the sodium alginate solution and mix well, at this time the charge density of polyanionic sodium alginate and polycationic polyethyleneimine 1:1, slowly add 150 μL, 10% calcium chloride solution to cross-link to obtain sodium alginate-polyethyleneimine hydrogel, and the mea...

Embodiment 2

[0060] Example 2: Preparation of Sodium Alginate-Linear Polyethyleneimine Polyelectrolyte Hydrogel

[0061] Dissolve 1 g of sodium alginate powder (molecular weight 300 kDa) in 100 mL of ultrapure water and stir overnight to obtain a 1% sodium alginate solution as a polyanionic polymer solution with a measured charge density of 0.68 mmol / g. 1 g of linear polyethyleneimine (molecular weight 1.8 kDa) was dissolved in 100 mL of ultrapure water to prepare a polyethyleneimine solution with a mass fraction of 1% as a polycationic polymer solution, and the measured charge density was 2.27 mmol / g.

[0062] Take 1mL of sodium alginate solution into a small beaker, add 0.3mL of polyethyleneimine solution dropwise into the sodium alginate solution and mix well, at this time the charge density of polyanionic sodium alginate and polycationic polyethyleneimine is 1 : 1, slowly add 150 μ L, 10% calcium chloride solution for cross-linking to obtain sodium alginate-polyethyleneimine hydrogel, ...

Embodiment 3

[0063] Embodiment 3: Preparation of hyaluronic acid-quaternized chitosan polyelectrolyte hydrogel

[0064] 2 g of hyaluronic acid powder (molecular weight 250 kDa) was dissolved in 100 mL of ultrapure water and stirred overnight to obtain a 2% hyaluronic acid solution as an anionic polymer solution with a charge density of 0.13 mmol / g. Dissolve 0.1 g of quaternized chitosan (grafting degree 40% to 50%, molecular weight 100 kDa) in 100 mL of ultrapure water to prepare a quaternized chitosan solution with a mass fraction of 0.1% as a polycationic polymer Solution, the measured charge density is 10.6mmol / g.

[0065] Take 1mL hyaluronic acid solution and add it to a small beaker, add 0.025mL quaternized chitosan solution dropwise into the hyaluronic acid solution and mix well, at this time the polyanionic hyaluronic acid and polycationic quaternized chitosan The charge density was 1:1, and the hyaluronic acid-quaternized chitosan hydrogel was obtained with a zeta potential of -5m...

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Abstract

The invention relates to anti-bioadhesion polyelectrolyte gel as well as a preparation method and application thereof. The preparation method comprises the steps of uniformly mixing one or several polyelectrolyte polymer solutions with opposite charges in a charge ratio of 1 to 1, and generating gel by virtue of a physical crosslinking method, or a chemical crosslinking method or the combination of two methods, wherein positive charges and negative charges are balanced, and the form gel is electroneutral. The preparation method of the polyelectrolyte gel is simple and easy to operate, and theprepared gel has good protein adsorption resistance, is high in water content, does not cause inflammatory reaction and is beneficial to the mass transfer between a transplant and an organism. Therefore, the polyelectrolyte gel can be widely applied to the biomedical fields, particularly can be used for treating diabetes mellitus through packaging of islet cells and can be used as a drug release carrier, a wound dressing, a tissue repair scaffold material and the like.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a biocompatible hydrogel material composed of oppositely charged polyelectrolyte macromolecules and having anti-bioadhesion properties. The invention also relates to a preparation method of the hydrogel and its application in the biomedical field. Background technique [0002] With the rapid development of life science and material science, biomedical materials have become one of the effective tools for human to fight against diseases. For example, implantable medical devices (implantable sensors) can quickly and conveniently monitor various health data in a timely manner with high accuracy; tissue engineering scaffold materials can be combined with tissue cells to replace damaged tissues or organs of patients, and provide therapeutic tissue for human beings. Damage provides a new option; drug release carriers can target and efficiently release drugs at the lesion...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/36A61K47/34A61K35/39A61P3/10A61L27/20A61L27/38A61L27/52A61L27/50A61L26/00
CPCA61K9/06A61K35/39A61K47/34A61K47/36A61L26/0052A61L26/0061A61L26/008A61L27/20A61L27/3834A61L27/50A61L27/52A61L2430/06C08L5/08C08L5/04
Inventor 张雷张嘉敏朱迎男杨静
Owner TIANJIN UNIV
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