A method for preparing bioactivity possessed artificial cornea

Abstract

The invention relates to a method for preparing biologically active artificial cornea. It takes animal cornea basic material as raw material, promoting cornea growth or / and preventing partial lesion by enzyme slaking, repeated unfreezing, washing, and irradiating with 60Co. It employs physical and chemical method to remove cell component and soluble protein which will cause immune response, and retains external base material construction, adds multifunctional component which is favor for cornea cell growth or / and preventing partial lesion, freeze dries and stores it. The tensile strength and diopter of prepared artificial cornea are similar to that of normal cornea, it can prevent lesion and dissolution after being implanted in, and promote cornea epithelium regeneration and collagen synthesis; the biocompatibility is good, no obvious immune rejection reaction and no toxic to cell, and can be used to treat various eye injury.

Description

technical field [0001] The invention belongs to the technical field of artificial organs of biological materials, and in particular relates to a preparation method of an artificial cornea with biological activity. Background technique [0002] Corneal inflammation, trauma, chemical burns, congenital abnormalities and other diseases often lead to corneal damage, vision loss and even blindness. Corneal transplantation is the most effective clinical solution, but the source of allogeneic corneal donors is seriously lacking, and transplantation Before that, matching is required, which greatly restricts the development of corneal transplantation, and prevents a large number of patients with corneal damage from receiving timely treatment, eventually leading to blindness. In my country, about 300,000 patients become blind due to corneal damage every year, while only over 2,000 patients can receive corneal transplantation. In some special cases, such as chemical burns, ocular pemph...

AI Technical Summary

Problems solved by technology

The problem is that the cornea of ​​the xenogeneic animal used has been decellularized, only the corneal endothelial cells and stromal cells have been removed, and the immune rejection caused by the bioincompatibility of the remaining xenogeneic matrix protein components has not been considered; at the same time, during the preparation process A variety of detergents are used, and their unavoidable residues will produce toxicity to the cells and affect the clinical treatment effect; and the cornea treated by the invention does not contain some functional components that inhibit the development of local lesions and are beneficial to the growth of corneal cells. It is difficult to obtain the ideal restoration effect

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