26 results about "Gangliocyte" patented technology

The invention provides an optic nerve regulation and control system based on an ultrasonic technology, a first ultrasonic module is deployed on the outer side of a target human eyelid and is provided with an Archimedes spiral type first ultrasonic array facing the human eye, and the first ultrasonic array is configured to generate a gathered ultrasonic beam which acts on a human eye retina ganglion cell area in a targeted manner; the positioning module is deployed on the outer side of a target human eyelid and is configured to measure three-dimensional geometric data when a human eyeball horizontally faces the front and measure the depth distance of a human eye retina ganglion cell area in the human eye; the optic nerve response prediction module is pre-trained with an optic nerve response prediction model facing a target human body, and the optic nerve response prediction model is configured to adjust sound intensity output of the first ultrasonic array based on stimulation feedback prediction of human eye retina ganglion cells. According to the invention, the optic nerve regulation and control system satisfies non-intrusive, high-precision and self-adaptive requirements, and has optic nerve regulation and control and repair functions.

The invention discloses Hangzhou white chrysanthemum-derived exosome-like nano-vesicles, a preparation method thereof and application of the nano-vesicles in treatment of optic nerve retinal degeneration diseases. In-vitro experiments show that the Hangzhou white chrysanthemum-derived exosome-like nano-vesicles provided by the invention can effectively inhibit glutamic acid-induced retinal cell oxidative stress and mitochondrial injury and improve the cell activity. In-vivo experiments show that the survival rate of ganglion cells in the retina of an NMDA injury model mouse can be remarkably improved by injecting the Hangzhou white chrysanthemum-derived exosome-like nano-vesicle into a vitreous cavity, and the visual electrophysiological function of the NMDA injury model mouse can be improved. The invention provides a new thought and method for treatment of optic nerve retinal degeneration diseases, and has a wide application prospect. In addition, the preparation method of the Hangzhou white chrysanthemum-derived exosome-like nano-vesicle provided by the invention is simple, and has the advantages of low cost, wide applicability, good safety, no animal-derived components and the like.

The present invention provides use of lifitegrast in the preparation of a drug for treating retinal artery occlusion injury. Lifitegrast can effectively reduce retinal artery occlusion injury and apoptosis of retinal ganglion cells and has the effect of inhibiting inflammatory infiltration in the retina and activation of microglia. Moreover, lifitegrast has the effect of reducing retinal ganglion cell injury. Lifitegrast can be used for related diseases such as neuroinflammatory injury caused by retinal artery occlusion.

Viral constructs and methods of their use in the treatment of diseases and disorders mediated by reduced ELP1 expression are provided. The viral constructs include a coding sequence for human ELP1 under control of a heterogenous promoter (such as a small nuclear RNA promoter), allowing for an increase in ELP1 protein expression in neuron cells of the central nervous system, including for instance retinal cells. Methods of using such viral constructs to protect neural cells, for instance by reducing death of retinal ganglion cells, are also provided.

This invention discloses an injectable hydrogel for long-term sustained release of NGF and CNTF, its preparation method, and its application in visual system diseases. The bioactive factor sustained-release hydrogel of this invention comprises a hydrogel matrix and bioactive factor microspheres loaded on the matrix; the raw materials for preparing the hydrogel matrix include chitosan, type II collagen, and hyaluronic acid; the bioactive factor microspheres comprise an inner core and a capsule wall, the inner core being a bioactive factor selected from one or both of ciliary neurotrophic factor and nerve growth factor, and the capsule wall being a polylactic acid-glycolic acid copolymer. This hydrogel can achieve long-term stable release of NTF, activating endogenous neural stem cells in the retina to generate new retinal ganglion cells. It exhibits high safety, good injectability, and a long sustained-release period, and can be used to prepare drugs for treating visual system diseases such as glaucoma, optic nerve damage, and macular degeneration.

The invention discloses application of upatinib in preparation of a medicine for treating acute retinal artery occlusion, and belongs to the field of biological medicine. Experimental verification shows that upatinib can remarkably relieve retina ischemia reperfusion injury caused by RAO and improve the survival and functional recovery of ganglion cells; by inhibiting JAK / STAT pathway mediated inflammatory cascade reaction, microglial cell M1 type polarization can be regulated, retinal inflammatory response can be inhibited, proinflammatory factor transcription and inflammatory diffusion can be reduced, and retinal nerve cell injury after RIR can be improved. The invention not only provides a new drug strategy of a targeting molecular mechanism for RAO treatment, but also provides a new theoretical basis for drug redevelopment and precise treatment of ischemic retinopathy, and has a wide application prospect.

This invention discloses an extracellular vesicle derived from Gastrodia elata, its preparation method, and its use in preparing drugs for treating glaucoma, belonging to the field of biomedical technology. This invention claims protection for an extracellular vesicle prepared from dried Gastrodia elata. The extracellular vesicles provided by this invention have no significant cytotoxicity and good biocompatibility. Studies have shown that these extracellular vesicles can significantly inhibit apoptosis of R28 cells and loss of Brn3a⁺ cells in the mouse retina in a glutamate excitotoxicity model. In a glaucoma model, these extracellular vesicles can effectively protect the survival of retinal ganglion cells (RGCs) and retinal function, and can significantly reduce retinal neuroinflammatory responses in the glaucoma model. Therefore, the extracellular vesicles provided by this invention have a clear anti-glaucoma-related retinal damage effect and possess great potential for development into drugs for treating glaucoma.

The present invention relates to reducing an activation of a trigeminal ganglion neuron or cell and related uses in treating or preventing migraine.

The application relates to a rapamycin-G4H nanocapsule, a preparation method and application thereof. The preparation method of the rapamycin-G4H nanocapsule comprises the following steps: S1. preparing a rapamycin micelle by adopting a film hydration method; S2. modifying a dibenzocyclooctyne amplification primer on the surface of the rapamycin micelle through a click chemistry reaction to obtain a rapamycin micelle modified with the amplification primer; S3. taking a linear DNA molecule as a template, and synthesizing a circular amplification template through an intramolecular cyclization reaction; and S4. incubating the circular amplification template, the rapamycin micelle modified with the amplification primer, a thermophilic DNA polymerase, a pyrophosphatase, dNTP and Co-G4-haem DNAzyme under the condition of a thermophilic DNA polymerase reaction buffer. The rapamycin-G4H nanocapsule can play an efficient antioxidant stress and immune inflammatory regulation function, can improve the intraocular microenvironment of a glaucoma patient, and can inhibit the continuous apoptosis of retinal ganglion cells.

The invention belongs to the field of biological medicines, and relates to a new pharmaceutical application of miR-126-3p. The invention discloses application of miR-126-3p or a functional equivalent thereof in preparation of a medicine for preventing or treating retinal structure damage and / or retinal function damage caused by retinal ischemia reperfusion injury caused by retinal artery occlusion. According to the medicine, by supplementing endogenous miR-126-3p consumed in the RIRI process, ganglion cell death can be reduced, and the retina structure can be protected; meanwhile, retina electrophysiological signals can be recovered, the retina function is improved, and therefore the core problem that in the prior art, vision of RAO patients is difficult to recover after blood recanalization is solved.

This application discloses a display adjustment method and a display. The display adjustment method includes: obtaining a target biological cycle; determining a first target light intensity corresponding to a target blue light source in the display based on the target biological cycle; the light generated by the target blue light source acting on biological retinal ganglion cells; and adjusting the parameters of the display based on the first target light intensity so that the brightness, chromaticity, and display color of the display meet the target display effect and are adapted to the target biological cycle.

The application provides an application of YAP in preparation of a drug for preventing or treating retinal ganglion cell injury, and proves that up-regulation of YAP expression or activity can significantly reduce the retinal oxidative stress level, inhibit the neuroinflammatory response, and reduce the number of RGC death, thereby effectively protecting the nerve function. The application provides a new treatment target for diseases characterized by RGC injury, such as traumatic optic neuropathy and glaucoma, and YAP or an expression promoter thereof can be used for preparing a drug for preventing, relieving and / or treating the diseases, and has an important clinical application prospect.

The invention provides application of kushenone in preparation of a product for preventing and / or treating glaucoma, and belongs to the technical field of biological medicine. According to the application disclosed by the invention, the kushenone has the effect of preventing and / or treating glaucoma for the first time, can be used as a candidate medicine for treating acute glaucoma, can prevent ischemia reperfusion injury caused by acute intraocular pressure rise, and has the effect of inhibiting ferroptosis so as to play a role in protecting retinal ganglion cells; an effect can be achieved by inhibiting a cGAS-STING pathway and LCN2 expression, so that ferroptosis of microglial cells is inhibited.

This invention belongs to the field of biotechnology, specifically disclosing a cRGD peptide-functionalized extracellular vesicle derived from mesenchymal stem cells, its preparation method, and its applications. The extracellular vesicle surface is anchored to the cRGD peptide via membrane fusion technology, enabling active targeting and activation of microglia. The preparation method includes isolating and culturing mesenchymal stem cells, purifying natural vesicles by differential ultracentrifugation, and then co-incubating with DSPE-PEG-cRGD for membrane insertion modification. In vitro and in vivo experiments show that the vesicles can be efficiently taken up by activated microglia, delivering endogenous miRNAs, inhibiting the NF-κB pathway, and promoting the transformation of microglia from the M1 to the M2 phenotype. In a retinal ischemia / reperfusion injury model, intravitreal injection of the vesicles reduces retinal ganglion cell apoptosis, protects retinal structure and optic nerve integrity, and restores electroretinogram function. This invention can be used to prepare drugs to alleviate neuroinflammation caused by acute glaucoma.

The application discloses sgRNA targeting carbonic anhydrase 2 and application thereof. The sgRNA targeting carbonic anhydrase 2 comprises sgRNA as shown in SEQ ID NO. 1 and sgRNA as shown in SEQ ID NO. 2. The application develops sgRNA for double-site knockout of ciliary body Car2 based on CRISPR-Cas9 and AAV technology, realizes efficient Car2 targeting, has high knockout efficiency, effectively inhibits aqueous humor production, has obvious effect of reducing intraocular pressure and long maintenance time, significantly reduces retinal ganglion cell apoptosis, can be used for glaucoma treatment, and has wide application prospect.

The present application belongs to the field of biological medicine research, and specifically provides the use of a transcription factor in one or more of the following: preparation of a retinal disease treatment drug; preparation of a retinal ganglion cell regeneration product; preparation of a product for inducing reprogramming of adult cells into retinal ganglion cells; preparation of a retinal ganglion cell; the transcription factor is selected from any one or more of Brn3B, Sox4, Atoh7, Sox11 and Isl1. The cell material used for the regenerated retinal ganglion cells is the endogenous cells of the self, and compared with the cell transplantation, there is no risk of immune rejection and tumorigenesis; the use of viral expression genes is simple and easy to popularize.

The invention discloses a nano-drug for enhancing the transplantation curative effect of stem cell-derived retinal ganglion cells as well as a preparation method and application of the nano-drug. Lithium ions and epicatechin gallate are self-assembled to form a lithium-epicatechin gallate nano-drug; the lithium-epicatechin gallate nano-drug is stable in structure and has strong oxidation and mitochondrial protection effects, the lithium-epicatechin gallate nano-drug and the epicatechin gallate nano-drug have synergistic interaction in function, and the lithium-epicatechin gallate nano-drug not only has dual nerve protection advantages, but also can improve drug stability and treatment effect; furthermore, the lithium-epicatechin gallate nano-drug and the embryonic stem cell-derived ganglion cells are transplanted in a combined manner, so that the survival rate and the neurite protection of the nano-drug under the oxidative stress condition can be remarkably improved, and more effective neuroprotection and functional reconstruction are realized in treatment of retina degenerative diseases such as glaucoma and the like.

The invention discloses a conductive polypyrrole nano-composite based on polydopamine modification, a preparation method of the conductive polypyrrole nano-composite and application of the conductive polypyrrole nano-composite in preparation of a medicine for treating retinal ischemia reperfusion injury, and relates to the technical field of biomedical nano-materials. The polydopamine-modified conductive polypyrrole nano-composite forms a core-shell structure through copolymerization of dopamine and pyrrole monomers, and has the properties of oxidation resistance, iron ion chelation, inflammation resistance and conductivity. The nanocomposite can effectively remove active oxygen, inhibit ferroptosis of retinal ganglion cells, regulate polarization of microglial cells to M2 anti-inflammatory phenotype, and recover the endogenous electrophysiological microenvironment of the retina. In a retina ischemia reperfusion injury model, retina structure injury and function loss are remarkably relieved by injecting P-PPy, and good biocompatibility is shown; and a comprehensive and reliable technical route and evaluation standard are provided for application of the nano-composite in preparation of the medicine for treating the retinal ischemia-reperfusion injury.

The invention provides application of an itaconic acid compound in preparation of a medicine for treating retinal artery occlusion, and belongs to the technical field of treatment of fundus diseases. In particular to new application of itaconic acid and derivatives thereof in preparation of drugs for treating or preventing retinal artery occlusion, the technical cognition of itaconic acid compounds in the traditional application field is broken through, a new small molecule candidate drug is provided for prevention and treatment of retinal ischemic diseases, and the application value is definite. The damage to the structure of the retina in retinal artery occlusion can be relieved, and the visual function is effectively improved; the retina microglial cells can be promoted to be polarized to M2 type, the generation of anti-inflammatory factors IL-10 and TGF beta can be increased, and a protective effect is achieved in the inflammatory response of the retina; the effect of protecting retinal ganglion cells from apoptosis is also achieved. The compound can be used for related diseases such as retinal ischemic diseases, fundus retina inflammation injury and optic nerve injury, and has a wide application prospect for fundus diseases.

The utility model relates to the technical field of phototherapy, in particular to a light-operated blood glucose eyeshade device and a light-operated blood glucose eyeshade system. The light-operated blood glucose eyeshade device comprises a middle shell, an LED lamp panel and a rear shell. The middle shell and the rear shell are arranged on the two sides of the LED lamp panel. A plurality of LED lamp beads are arranged on the side, close to the rear shell, of the LED lamp panel. And the LED lamp panel is connected with the rear shell. And the middle shell is connected with the rear shell and covers the LED lamp panel. After a user correctly wears the eyeshade, red light emitted by the eyeshade can irradiate human eyes; red light enters from a visual window, signals can be directly transmitted by activating photosensitive retinal ganglion cells (ipRGCs) (special photoreceptor cells) through optic nerves to a series of nerve nuclei of hypothalamus and medulla oblongata, finally, the red light acts on peripheral brown adipose tissue BAT through sympathetic nerves, the metabolic capacity of blood sugar can be affected, and therefore the sugar control effect is achieved. And when the embodiment is used for reducing blood sugar, the user only needs to wear the equipment, so that the method is easy to implement.

This invention discloses an exosome-like nanovesicle derived from Hangzhou white chrysanthemum, its preparation method, and its application in the treatment of optic nerve and retinal degenerative diseases. In vitro experiments show that the exosome-like nanovesicles derived from Hangzhou white chrysanthemum provided by this invention can effectively inhibit glutamate-induced oxidative stress and mitochondrial damage in retinal cells, and enhance cell viability. In vivo experiments show that intravitreal injection of these exosome-like nanovesicles derived from Hangzhou white chrysanthemum can significantly improve the survival rate of ganglion cells in the retina of mice with NMDA damage and improve their visual electrophysiological function. This invention provides a new approach and method for the treatment of optic nerve and retinal degenerative diseases, and has broad application prospects. Furthermore, the preparation method of the exosome-like nanovesicles derived from Hangzhou white chrysanthemum provided by this invention is simple and has advantages such as low cost, wide applicability, good safety, and absence of animal-derived components.

The application discloses a spectrum parameter determination method suitable for regulating sugar metabolism, and comprises the following steps: exposing each of a plurality of to-be-tested objects to a plurality of light stimulation modes respectively; for each of the light stimulation modes, performing a blood sugar tolerance test on the plurality of to-be-tested objects respectively after a preset exposure time, so as to obtain first blood sugar tolerance information of the plurality of to-be-tested objects; determining second blood sugar tolerance information corresponding to the plurality of light stimulation modes respectively according to the first blood sugar tolerance information of the plurality of to-be-tested objects; and determining a spectrum parameter corresponding to a light stimulation mode with the optimal blood sugar tolerance improvement capability as a target spectrum parameter according to the second blood sugar tolerance information of the plurality of light stimulation modes. The application is based on the spectrum sensitivity of self-photoreceptor ganglion cells, and the target spectrum parameter capable of improving sugar metabolism is obtained by using the blood sugar tolerance test, so that an implementable light-providing scheme can be provided for effectively improving sugar metabolism.

This invention discloses a method for constructing an inducible CaMKIIα-initiated cell line and its applications. The inducible CaMKIIα-initiated cell line is obtained by knocking in the DNA sequence shown in SEQ ID No. 2 (positions 861-5336 from the 5' end) into the AAVS1 safe harbor site of the human embryonic stem cell genome. Based on a strategy combining the Tet-on system and the CRISPR / Cas9 system, this invention constructs a cell line in hESCs where tetracycline controls the CaMKIIα phosphorylation switch. This method is precise, controllable, stable, and low-cost, and is of great significance for studying CaMKIIα function. Using this cell line, it was found that initiating CaMKIIα can promote axonal regeneration in ganglion cells. This invention provides a useful tool for studying the pathogenesis and treatment of nervous system diseases and has significant application value.

Provided are a method for using a white light source, and a white light source, wherein precise control of daily rhythm and pleasant lighting can be safely and easily realized in a place of living such as a general home. An embodiment provides a method for using a white light source. Provided that: amounts of stimulation light emitted from a white light source to the intrinsically photosensitive retinal ganglion cells (ipRGCs) and visual cells of the L cone and M cone, among the visual cells of human, are defined as a melanopic irradiance, an L-cone opic irradiance, and an M-cone opic irradiance, respectively; a ratio of the amounts of the stimulation light is expressed by the following formula (1); and A is the ratio corresponding to the emission spectrum of the white light source, and B is the ratio corresponding to the radiation spectrum of a black body having the same color temperature as the white light source, an amount of stimulation light emitted to the ipRGC is quantitatively changed by changing the emission intensity of the white light source satisfying the following formula (2). Melanopic irradiance / (L-cone opic irradiance+M-cone opic irradiance) (1) 0.88≤A / B≤1.11 (2)

Nucleic acid molecules and compositions, and methods using the same are provided herein for intraocular gene-based delivery and expression of two or more proneural bHLH transcription factors in the retina. The nucleic acid molecules, compositions and methods disclosed herein stimulate regeneration of retinal interneurons from retinal Müller glia (MG) and reprogram the MG into bipolar, amacrine, horizontal, and / or ganglion cells. Such methods and nucleic acid molecules are used for vision restoration and / or treatment of a range of ocular diseases involving retinal degeneration after injury, disease, or vison loss.

This invention discloses a method for obtaining ganglion cells through induction and enrichment of human embryonic stem cells. The method includes the following steps: knocking in genes encoding cell membrane markers and fluorescent proteins into the genome of human embryonic stem cells to obtain a reporter cell line; subjecting the reporter cell line to three-dimensional induction to obtain human retinal organoids; and sorting ganglion cells endogenously expressing cell membrane markers and fluorescent proteins from the human retinal organoids. This application provides an efficient method for obtaining ganglion cells and has significant application value.