30results about How to "Maintain spatial structure" patented technology

The invention discloses a biomimetic specific immune adsorption material with PAMAM (Polyamidoamine) as a spacer arm, and a preparation method and the application thereof. The preparation process comprises the following steps of: firstly, obtaining sepharose gel azide by reaction of agarose epoxide and sodium azide; preparing a series of PAMAM dendrimer-like polymers in different generations by repeated addition reaction with methyl acrylate and amination reaction with ethidene diamine; after preparing amino acid methyl ester hydrochloride with the amino acid as the raw material, modifying the PAMAM dendrimer-like polymers with the amino acid methyl ester hydrochloride to prepare the amino acid modified PAMAM; coupling the amino acid modified PAMAM with sepharose gel azide through a click reaction so as to obtain the biomimetic specific immune adsorption material with PAMAM as the spacer arm. The material disclosed in the invention is prepared by simple synthetic steps, and the preparation is safe; products have the characteristics of strong specificity, high adsorbing efficiency to the immunoglobulin and excellent regeneration performance, and can be used for IgG (immunoglobulin G) separation and clinical immune adsorption treatment.

The invention provides an extended target tracking method based on combination of skeleton characteristic points and distribution field descriptors. The extended target tracking method comprises the following steps: firstly, preprocessing images to be processed by using Gaussian smooth filtering to remove influence of noise on a subsequent algorithm; and subsequently, cutting the smoothed images by using a fuzzy C-means clustering algorithm FCM to obtain binary images, carrying out Hough transform on the cut and obtained binary images to detect a part with an obvious linear characteristic on the target, extracting characteristic points from a part without obvious linear characteristic on the target by using a skeleton, then carrying out linear fitting on the characteristic points on the skeleton to obtain a target axis, taking a intersection point of the straight lines of the obtained axis as an initial track point, then calculating a distribution field in a certain neighborhood field of the point and searching a best position in the neighborhood field when a next frame comes, so as to obtain the current best track point. Through the extended target tracking method, the fuzziness problem is solved; and the extended target tracking method is capable of stably tracking the extended target under a condition with a great posture change.

The invention discloses a bionic specific immunity adsorbing material and a preparation method as well as application thereof. The preparation method comprises the following steps of: reacting epoxidation agarose with sodium azide to obtain agarose azide gel; dissolving succinic anhydride into methylene dichloride and propargyl alcohol and adding a catalyst to prepare acetylenic acid; dissolving the acetylenic acid and N-hydroxysuccinimide into 1,4-dioxane, and then adding a condensing agent to prepare active ester of the acetylenic acid; dissolving the active ester of the acetylenic acid into the 1,4-dioxane and then mixing with aqueous solution of aglycone to prepare ethynylation amino acid; dissolving solution of the ethynylation amino acid into agarose azide; and adding copper sulfate pentahydrate and sodium ascorbate as catalysts to prepare a product. According to the bionic specific immunity adsorbing material and the preparation method as well as application thereof, the agarose gel which is rich in hydroxyl groups and has good blood compatibility and mechanical property is used as a carrier, synthesis steps are simple, the preparation is safe, and a product has the characteristics of high specificity, high adsorption efficiency on IgG (Immunoglobulin G) and good regeneration performance and can be used for IgG separation and clinical immunoadsorption treatment.

The invention provides a bionic immune adsorbent using PAMAM (polyamidoamine) as a spacer arm. The bionic immune adsorbent has the following chemical structure of Gel-PAMAM-AA as shown in the specification, wherein Gel represents a hydroxy-containing hydrophilic carrier; AA represents amino acid; n is 0, 1, 2 or 3; and PAMAM is polyamidoamine dendrimer, the hydrophilic carrier is agarose, cellulose or polyvinyl alcohol, and the amino acid is L-histidine, phenylalanine or tryptophan. The invention also provides a preparation method of the bionic immune adsorbent. By using the dendric macromolecular PAMAM as a spacer arm, the outer surface of the carrier has more active sites for coupling more ligands; by adjusting the position and direction of a triazole ring, the triazole ring is farther from the dendric PAMAM, so that adsorption of target substances is facilitated, and the adsorbent has specific adsorption; and the cost is low, the process is simple, and the reaction condition is mild.

The invention discloses a preparation method of an immobilized enzyme nanofiber membrane for detecting the alcohol content in spit. The preparation method comprises the steps of: covalently binding alcohol oxidase through a polymer such as polyethylene glycol or polyethyleneimine as an arm molecular multi-site; further fixing a plurality of subunits of the alcohol oxidase by the polyethyleneimine, polylysine, poly-histidine or chitosan and the like through physical action among charges; then absorbing indigo blue as a chromogenic substrate; generating hydrogen peroxide after ethanol in a sample is oxidized by the alcohol oxidase in detection; leading the indigo blue to fade by the hydrogen peroxide, and conveniently calculating the ethanol concentration in the sample by comparing the fading degree of the indigo blue with a standard color atla. The preparation method is high in sensitivity, simple and convenient to operate, and low in price, can be repeatedly used for a plurality of times, and can be conveniently used for investigating and treating drunk driving by a law-enforcing department; and the reliable evidence is provided for the law enforcement process.

The invention discloses a traditional Chinese medicine composition for treating cerebral apoplexy, which comprises the active components containing the following raw materials by ratio: 70-100 parts of gen-seng, 450-550 parts of fresh lumbricus, 200-300 parts of fresh leech, 200-300 parts of fresh ground beetle and 70-100 parts of pseudo-ginseng. The present invention also discloses a preparationmethod of the traditional Chinese medicine composition. The method avoids high temperature, high pressure, strong acid, strong base and an organic solvent treatment in the traditional technology during the preparation process, maximally keeps the space structure and the physiological activity of the active substances as well as ensures the curative effects of the traditional Chinese medicine composition.

The invention discloses a low-temperature preservation method of blood vessels. The low-temperature preservation method comprises the following steps: separating to obtain fresh blood vessel tissues,pouring a cryoprotectant into the blood vessels, putting the blood vessels into a cryoprotectant or a cryoprotectant containing magnetic nanoparticles, putting an obtained mixture into a cryopreservation tube or a cryopreservation bag, sealing, and carrying out programmed cooling; wherein the cryoprotectant comprises the following components 5%-8% of hydroxyethyl starch, 10%-15% of dimethyl sulfoxide, 0.01%-0.02% of a surfactant and the balance a culture medium. The invention also discloses a rewarming method of blood vessels. The rewarming method of blood vessels comprises the following steps: taking out a blood vessel sample cryopreserved by using the cryoprotectant containing the magnetic nanoparticles from liquid nitrogen, placing the blood vessel sample in an alternating magnetic field area, and carrying out magneto-thermal rewarming under the conditions that the magnetic field frequency is 764.8 kHz, the magnetic field intensity is 250-300Gs and the time is 40-60s. According to the blood vessel cryopreservation method, blood vessel breakage or space structure damage caused by different cooling rates of the temperature inside and outside the blood vessels is avoided, the mechanical property of the blood vessels is protected from being affected as far as possible, operation is easy, and efficiency is high.

The invention is applicable to the technical field of biochemistry, in particular to a calibrator for a biochemical calibrator and the biochemical calibrator. Each liter of the matrix for the biochemical calibrator comprises the following components of 10 to 100 mmol of a pH regulator, 2-50 g of a saccharide protective agent, 2-100 g of a polymer protective agent, 5-50 g of a protein protective agent, 0.5 to 5 grams of surfactant, 5 to 20 millimoles of amino acid, 0.02 to 50 millimoles of antioxidant, 0.05 to 2 millimoles of protease inhibitor, 5 to 30 grams of alcohol organic solvent, 0.2 to0.5 gram of preservative, 8.5 to 9.5 grams of sodium chloride and 0.1 to 5 millimoles of magnesium salt and/or zinc salt. According to the calibrator for the biochemical calibrator, when the analysiscomponents are added into the matrix for the biochemical calibrator, the components in the matrix for the biochemical calibrator can play a better role in protecting the analysis components, particularly, the structural variation caused by water loss of the analysis components in the freeze-drying process can be avoided, and the effect of the analysis components is effectively reserved.

The invention provides a temperature-sensitive polypeptide encapsulated mesoporous silica intelligent drug carrier and a preparation method thereof. The temperature-sensitive polypeptide encapsulated mesoporous silica intelligent drug carrier is formed by packaging temperature-sensitive polypeptide and mesoporous silica. Two polypeptides with different sequences are provided, the secondary structures of the polypeptides change along with temperature rise, the polypeptides have relatively mild phase change temperature, and the polypeptides are modified to the surface of a mesoporous nano material through disulfide bonds formed by sulfydryl of cysteine, so that drug encapsulation is realized. The polypeptides can extend a polypeptide chain to realize drug diffusion when the temperature rises, and the disulfide bond can be selectively cracked by reducing reagents GSH, TCEP, DTT and the like. Along with the change of the temperature and the concentration of glutathione, targeted drug release can be realized at a diseased region, or the glutathione can be used for directional release in other fields.

The invention provides a hyaluronic acid inhalant. The hyaluronic acid inhalant comprises oligo hyaluronic acid or a salt thereof and oligomeric hyaluronic acid or a salt of oligomeric hyaluronic acid, the mass content of oligo hyaluronic acid or salt thereof is 0.01%-5%, preferably 0.3%-2%, and the mass content of oligomeric hyaluronic acid is 0.01%-5%, preferably 0.1%-1%. The hyaluronic acid inhalant prepared by the invention can be widely applied to respiratory diseases such as rhinitis, pharyngitis, bronchitis, asthma, cough, chronic obstructive pulmonary disease and the like. The hyaluronic acid inhalant increases viscosity of medicines, prolongs the retention time of effective components of the medicines in nasal mucosa, improves the bioavailability of the medicines, is beneficial tomaintaining the space structure of pulmonary elastic fibers and preventing the deterioration of diseases, can effectively treat obstructive chronic lung diseases and reconstruct lung functions, and can also effectively prevent recurrence of lung diseases. The hyaluronic acid inhalant obtained by combining hyaluronic acid with a low molecular weight and oligomeric hyaluronic acid.