30results about How to "Maintain spatial structure" patented technology

The invention discloses a recombinant varicella zoster virus vaccine, which comprises an amino acid sequence of a recombinant glycoprotein gE extracellular region of a live attenuated VZV strain (OKA strain) gene and a fusion protein formed by a human immunoglobulin Fc segment, and further comprises preparation and an application of the fusion protein, and a corresponding recombinant gene, an eukaryotic expression vector and the like. The fusion protein provided by the invention has good immunogenicity, and can induce generation of a high-level serum neutralizing antibody.

The invention discloses a preparation method for metal ion printing chitosan porous micro-spheres and belongs to the technical field of parting material preparation. The preparation method comprises the following steps of: using an acetic acid aqueous solution to dissolve chitosan and metal salt to serve as a dispersion phase, using an organic solvent immiscible to water to dissolve a surface active agent and a cross-linking agent to serve as a continuous phase, using a shear action of the continuous phase to disperse the dispersion phase to form micron order liquid drops in microstructural equipment, enabling the liquid drops to complete crosslinking and solidification to form micro-spheres after certain retention time, finally eluting the organic solvent, the surface active agent, uncreated cross-linking agent, the metal ion and the like in the micro-spheres, and drying to obtaining the metal ion printing chitosan porous micro-spheres. The method is simple, convenient and rapid, and prepared metal ion printing chitosan porous micro-spheres are even in size, keep good network structures and effective adsorption position points and have the characteristics of big adsorption capacity, high speed and the like. The preparation method provides an effective means for mass preparation of high-performance metal ion adsorbing materials.

The invention discloses a structure-preserving interpolation method of two-dimensional shapes. The structure-preserving interpolation method comprises the following steps: respectively arranging two-dimensional shapes such as a 'source shape' and a 'target shape' in a computer; firstly, introducing a simple structure to represent the space layout relation between adjacent lines in the source shape, and naming the structure as a 'source space frame'; similarly, adopting similar structures to represent the space layout relation between adjacent lines in the target shape, and naming the structure as a 'target space frame'; and then interpolating the source space frame to the target space frame, and finally realizing natural transition from the source shape to the target shape. The structure-preserving interpolation method disclosed by the invention has the characteristic that not only can significant geometrical features of single lines be maintained, but also the space structure relation between the adjacent lines can be maintained, so that the semantic features of the source shape and the target shape can be maintained in a transition shape sequence, and finally a visually-beautiful shape transition sequence is generated.

The invention provides a cell stationary phase based on microcarrier cells and a preparation method of the cell stationary phase. Cells are cultured on a microcarrier surface; after being immobilized by paraformaldehyde, cells cultured by a microcarrier and the microcarrier supporting the growth of the cells are used as the stationary phase; a cell chromatographic column is prepared by wet-process packing. The method is applied to prepare the stationary phase, operation is simple, reactions are gentle and amplification is easy. The stationary phase has the characteristics of cells and keeps original base characteristics of the cells; a drug acceptor also keeps a base space structure; the cell stationary phase prepared by applying the method can be applied to screening drugs and improving drug screening specificity.

The invention relates to a new biological cellulose composite gel material and its new application in preparing wound dressing products. The composite gel provided by the invention is composed of biological cellulose hydrogel and protein thixotropic gel. Biocellulose hydrogel has high water content, good water retention and sufficient mechanical strength. The protein gel is formed in the biological cellulose spatial structure, which can maintain the spatial structure of the biological cellulose hydrogel and promote cell adhesion and proliferation. It can also form a protein thixotropic gel, which can be converted from a protein gel to a protein sol by mechanical pressing before or during use to further promote cell adhesion and proliferation, and slowly release globulin that has not been converted into a gel , to further improve the antibacterial and anti-inflammatory properties of the dressing, and promote wound healing.

The invention provides a bionic immune adsorbent using PAMAM (polyamidoamine) as a spacer arm. The bionic immune adsorbent has the following chemical structure of Gel-PAMAM-AA as shown in the specification, wherein Gel represents a hydroxy-containing hydrophilic carrier; AA represents amino acid; n is 0, 1, 2 or 3; and PAMAM is polyamidoamine dendrimer, the hydrophilic carrier is agarose, cellulose or polyvinyl alcohol, and the amino acid is L-histidine, phenylalanine or tryptophan. The invention also provides a preparation method of the bionic immune adsorbent. By using the dendric macromolecular PAMAM as a spacer arm, the outer surface of the carrier has more active sites for coupling more ligands; by adjusting the position and direction of a triazole ring, the triazole ring is farther from the dendric PAMAM, so that adsorption of target substances is facilitated, and the adsorbent has specific adsorption; and the cost is low, the process is simple, and the reaction condition is mild.

The invention relates to VR glasses with an eyeshade. The VR glasses comprise a main body, a connecting part is arranged on sides of the main body; a lens hole is formed in the connecting part; an eyeshade is arranged on one side, facing the lens hole, of the main body; the eyeshade comprises a first shell and a second shell; the first shell and the second shell are oppositely arranged; two soft supporting plates are arranged between the first shell and the second shell, one end of each soft supporting plate is fixedly connected with the first shell, the other end of each soft supporting plateis fixedly connected with the second shell, the section of each soft supporting plate is of a trapezoidal structure, and shading cloth is arranged between the first shell and the second shell. According to the VR glasses with the eyeshade, the two soft fixed supporting plates are arranged, so the whole eyeshade can keep an integral space structure, meanwhile, the comfortable feeling of the eyeshade can be guaranteed, the whole eyeshade is of a soft structure, a user can adjust the position of the eyeshade and the integral tightness degree according to the size of the face of the user, and therefore the user can obtain a better imaging effect.

The invention discloses a blood vessel cryopreservation method: separate and obtain fresh blood vessel tissue, perfuse a cryoprotectant into the blood vessel, place the blood vessel in the cryoprotectant or the cryoprotectant containing magnetic nanoparticles, and then place the cryoprotectant in the cryoprotectant. Store in a tube or cryopreservation bag, seal, and program cooling; the cryoprotectant is: 5% to 8% of hydroxyethyl starch, 10% to 15% of dimethyl sulfoxide, and 0.01% to 0.02% of surfactant , and the rest is medium. The invention also discloses a blood vessel rewarming method: the blood vessel sample cryopreserved by a cryoprotectant containing magnetic nanoparticles is taken out of liquid nitrogen, placed in an alternating magnetic field area, and subjected to magnetic-thermal rewarming. The parameters are: The magnetic field frequency is 764.8kHz, the magnetic field strength is 250-300Gs, and the time is 40-60s. The blood vessel cryopreservation method of the present invention does not cause blood vessel breakage or damage to the spatial structure due to different cooling rates of the inside and outside of the blood vessel, and can maximize the mechanical properties of the blood vessel without being affected, with simple operation and high efficiency.

The invention discloses a biomimetic specific immune adsorption material with PAMAM (Polyamidoamine) as a spacer arm, and a preparation method and the application thereof. The preparation process comprises the following steps of: firstly, obtaining sepharose gel azide by reaction of agarose epoxide and sodium azide; preparing a series of PAMAM dendrimer-like polymers in different generations by repeated addition reaction with methyl acrylate and amination reaction with ethidene diamine; after preparing amino acid methyl ester hydrochloride with the amino acid as the raw material, modifying the PAMAM dendrimer-like polymers with the amino acid methyl ester hydrochloride to prepare the amino acid modified PAMAM; coupling the amino acid modified PAMAM with sepharose gel azide through a click reaction so as to obtain the biomimetic specific immune adsorption material with PAMAM as the spacer arm. The material disclosed in the invention is prepared by simple synthetic steps, and the preparation is safe; products have the characteristics of strong specificity, high adsorbing efficiency to the immunoglobulin and excellent regeneration performance, and can be used for IgG (immunoglobulin G) separation and clinical immune adsorption treatment.

The invention discloses a tending method for promoting western sichuan subalpine natural secondary forest to restore by utilizing reserved trees. The method comprises the following steps: S1, selecting abies faxoniana, dragon spruce, hemlock, red birch and white birch as target tree species which need restoring in the secondary forest; S2, selecting the reserved trees; when the target tree species are the abies faxoniana, the dragon spruce or the hemlock, controlling the diameters at breast heights of the reserved trees to be more than or equal to 25cm; when the target tree species are the red birch or the white birch, controlling the diameters at breast heights of the residual trees to be more than or equal to 35cm; S3, dividing the restoration stage of the natural secondary forest according to the importance value of dominant tree species of community, wherein the restoration stage of the natural secondary forest is divided into a bushwood stage, a birch broad-leaved forest stage and a mixed broadleaf-conifer forest stage; S4, tending at the bushwood stage, tending at the birch broad-leaved forest stage and tending at the mixed broadleaf-conifer forest stage. In the restoration process of the western sichuan subalpine natural secondary forest, the reserved trees are fully utilized to tend and manage the secondary forest, so that the adverse effects of felling to the service function of a forest ecosystem can be slowed down, update is promoted, and the time for enabling the secondary forest to be restored into a forest after felling is shortened.

The invention discloses a traditional Chinese medicine composition for treating cerebral apoplexy, which comprises the active components containing the following raw materials by ratio: 70-100 parts of gen-seng, 450-550 parts of fresh lumbricus, 200-300 parts of fresh leech, 200-300 parts of fresh ground beetle and 70-100 parts of pseudo-ginseng. The present invention also discloses a preparationmethod of the traditional Chinese medicine composition. The method avoids high temperature, high pressure, strong acid, strong base and an organic solvent treatment in the traditional technology during the preparation process, maximally keeps the space structure and the physiological activity of the active substances as well as ensures the curative effects of the traditional Chinese medicine composition.