30results about How to "Maintain spatial structure" patented technology

The invention provides a cell stationary phase based on microcarrier cells and a preparation method of the cell stationary phase. Cells are cultured on a microcarrier surface; after being immobilized by paraformaldehyde, cells cultured by a microcarrier and the microcarrier supporting the growth of the cells are used as the stationary phase; a cell chromatographic column is prepared by wet-process packing. The method is applied to prepare the stationary phase, operation is simple, reactions are gentle and amplification is easy. The stationary phase has the characteristics of cells and keeps original base characteristics of the cells; a drug acceptor also keeps a base space structure; the cell stationary phase prepared by applying the method can be applied to screening drugs and improving drug screening specificity.

The invention relates to VR glasses with an eyeshade. The VR glasses comprise a main body, a connecting part is arranged on sides of the main body; a lens hole is formed in the connecting part; an eyeshade is arranged on one side, facing the lens hole, of the main body; the eyeshade comprises a first shell and a second shell; the first shell and the second shell are oppositely arranged; two soft supporting plates are arranged between the first shell and the second shell, one end of each soft supporting plate is fixedly connected with the first shell, the other end of each soft supporting plateis fixedly connected with the second shell, the section of each soft supporting plate is of a trapezoidal structure, and shading cloth is arranged between the first shell and the second shell. According to the VR glasses with the eyeshade, the two soft fixed supporting plates are arranged, so the whole eyeshade can keep an integral space structure, meanwhile, the comfortable feeling of the eyeshade can be guaranteed, the whole eyeshade is of a soft structure, a user can adjust the position of the eyeshade and the integral tightness degree according to the size of the face of the user, and therefore the user can obtain a better imaging effect.

The invention discloses a blood vessel cryopreservation method: separate and obtain fresh blood vessel tissue, perfuse a cryoprotectant into the blood vessel, place the blood vessel in the cryoprotectant or the cryoprotectant containing magnetic nanoparticles, and then place the cryoprotectant in the cryoprotectant. Store in a tube or cryopreservation bag, seal, and program cooling; the cryoprotectant is: 5% to 8% of hydroxyethyl starch, 10% to 15% of dimethyl sulfoxide, and 0.01% to 0.02% of surfactant , and the rest is medium. The invention also discloses a blood vessel rewarming method: the blood vessel sample cryopreserved by a cryoprotectant containing magnetic nanoparticles is taken out of liquid nitrogen, placed in an alternating magnetic field area, and subjected to magnetic-thermal rewarming. The parameters are: The magnetic field frequency is 764.8kHz, the magnetic field strength is 250-300Gs, and the time is 40-60s. The blood vessel cryopreservation method of the present invention does not cause blood vessel breakage or damage to the spatial structure due to different cooling rates of the inside and outside of the blood vessel, and can maximize the mechanical properties of the blood vessel without being affected, with simple operation and high efficiency.

The invention discloses a tending method for promoting western sichuan subalpine natural secondary forest to restore by utilizing reserved trees. The method comprises the following steps: S1, selecting abies faxoniana, dragon spruce, hemlock, red birch and white birch as target tree species which need restoring in the secondary forest; S2, selecting the reserved trees; when the target tree species are the abies faxoniana, the dragon spruce or the hemlock, controlling the diameters at breast heights of the reserved trees to be more than or equal to 25cm; when the target tree species are the red birch or the white birch, controlling the diameters at breast heights of the residual trees to be more than or equal to 35cm; S3, dividing the restoration stage of the natural secondary forest according to the importance value of dominant tree species of community, wherein the restoration stage of the natural secondary forest is divided into a bushwood stage, a birch broad-leaved forest stage and a mixed broadleaf-conifer forest stage; S4, tending at the bushwood stage, tending at the birch broad-leaved forest stage and tending at the mixed broadleaf-conifer forest stage. In the restoration process of the western sichuan subalpine natural secondary forest, the reserved trees are fully utilized to tend and manage the secondary forest, so that the adverse effects of felling to the service function of a forest ecosystem can be slowed down, update is promoted, and the time for enabling the secondary forest to be restored into a forest after felling is shortened.

The invention discloses a traditional Chinese medicine composition for treating cerebral apoplexy, which comprises the active components containing the following raw materials by ratio: 70-100 parts of gen-seng, 450-550 parts of fresh lumbricus, 200-300 parts of fresh leech, 200-300 parts of fresh ground beetle and 70-100 parts of pseudo-ginseng. The present invention also discloses a preparationmethod of the traditional Chinese medicine composition. The method avoids high temperature, high pressure, strong acid, strong base and an organic solvent treatment in the traditional technology during the preparation process, maximally keeps the space structure and the physiological activity of the active substances as well as ensures the curative effects of the traditional Chinese medicine composition.