A preparation method of epigallocatechin gallate cross-linked small intestinal submucosa guided bone regeneration membrane

A technology of epigallocatechin and small intestinal submucosa, applied in tissue regeneration, medical science, surgery, etc., can solve the problems of poor mechanical properties, fast degradation speed, and poor space maintenance ability of bone regeneration membrane, and achieve good biophase Capacitance, slow degradation, and the effect of overcoming poor mechanical properties

Active Publication Date: 2020-05-15
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It overcomes the shortcomings of the existing small intestinal submucosa guiding bone regeneration membrane, such as poor mechanical properties, fast degradation speed, and poor space maintenance ability

Method used

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  • A preparation method of epigallocatechin gallate cross-linked small intestinal submucosa guided bone regeneration membrane
  • A preparation method of epigallocatechin gallate cross-linked small intestinal submucosa guided bone regeneration membrane
  • A preparation method of epigallocatechin gallate cross-linked small intestinal submucosa guided bone regeneration membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 A kind of preparation method of EGCG cross-linked SIS guided bone regeneration membrane

[0043] 1. Preparation method of SIS film:

[0044] (1) Cleaning and cutting: take a pair of fresh pig small intestines that were slaughtered within 4 hours, and only take the jejunum section, wash it repeatedly with a large amount of water, cut it longitudinally along the mesentery and cut it into 15cm-20cm intestine sections;

[0045] (2) Mechanical curettage: mechanically scrape off the serosal layer and muscular layer outside the cut intestinal segment, rinse it in normal saline for 3 times, each time for 5 minutes;

[0046] (3) Degreasing: Wring out the rinsed film, put it into 1000mL (methanol: chloroform volume ratio is 1:1) degreasing solution, put it in a fume hood for 12 hours, and replace the degreasing solution once in the middle;

[0047] (4) Rinse: Pour off the degreasing solution, rinse the degreasing film repeatedly with a large amount of flowing water ...

Embodiment 2

[0059] Example 2 A preparation method of EGCG cross-linked SIS-guided bone regeneration membrane

[0060] 1. Preparation method of SIS film:

[0061] Same as Example 1.

[0062] 2. EGCG cross-linked SIS film:

[0063] 1) Preparation of cross-linking solution: Weigh 0.25 g of EGCG powder and dissolve it in 100 mL of PBS solution to prepare a cross-linking solution with a concentration of 0.25%, and store in the dark;

[0064] 2) Cross-linking of SIS membrane: Put a piece of SIS membrane (150mg) into 25mL of the prepared cross-linking solution, and cross-link in the dark at 37°C for 48 hours;

[0065] 3) Cleaning of the cross-linked SIS membrane: repeatedly washing the cross-linked SIS membrane with PBS and deionized water; lyophilization and sterilization:

[0066] a. Single-layer material: lay the cross-linked SIS film flat, with the mucous membrane facing up, freeze-dry in a vacuum freeze dryer, and sterilize with ethylene oxide;

[0067] b. Double-layer material: Lay the...

Embodiment 3

[0069] Example 3 A preparation method of EGCG cross-linked SIS-guided bone regeneration membrane

[0070] 1. Preparation method of SIS film:

[0071] Same as Example 1.

[0072] 2. EGCG cross-linked SIS film:

[0073] 1) Preparation of cross-linking solution: Weigh 0.5 g of EGCG powder and dissolve it in 100 mL of PBS solution, prepare a cross-linking solution with a concentration of 0.5%, and store in the dark;

[0074] 2) Cross-linking of SIS membrane: Put a piece of SIS membrane (150mg) into 25mL of the prepared cross-linking solution, and cross-link in the dark at 37°C for 48 hours;

[0075] 3) Cleaning of the cross-linked SIS membrane: repeatedly washing the cross-linked SIS membrane with PBS and deionized water; lyophilization and sterilization:

[0076] a. Single-layer material: lay the cross-linked SIS film flat, with the mucous membrane facing up, freeze-dry in a vacuum freeze dryer, and sterilize with ethylene oxide;

[0077] b. Double-layer material: Lay the cro...

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Abstract

The invention discloses a material with bone repairing effect, which is small intestinal submucosal membrane crosslinked by epigallocatechin gallate, and its crosslinking index is 15.76%-21.44%. The present invention also provides the preparation method and application of the aforementioned materials. The material of the invention has good mechanical properties and slow degradation, can maintain the spatial structure of bone tissue regeneration, and has good biocompatibility and osteogenesis effect, and overcomes the existence of the existing small intestinal submucosa membrane as a guiding bone regeneration membrane Due to the disadvantages of poor mechanical properties, fast degradation rate and poor space maintenance ability, it has good clinical application prospects.

Description

technical field [0001] The invention relates to a preparation method of an epigallocatechin gallate cross-linked small intestinal submucosa guided bone regeneration membrane. Background technique [0002] Implant restoration has a high success rate and will not damage adjacent teeth, so it has become the first choice for the restoration of single missing teeth. For patients with multiple tooth loss and dentition loss, implant restoration also has a high success rate. Compared with traditional denture restoration, implant restoration has better stability, comfort and aesthetics, improves chewing efficiency, and greatly improves the quality of life of patients. The achievement and long-term maintenance of successful osseointegration is one of the most important prerequisites for successful implant restorations, namely the presence of sufficient healthy bone mass at the implant site. This does not only refer to sufficient bone height to accommodate implants of the appropriate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/00A61L31/14
CPCA61L31/00A61L31/005A61L31/14A61L31/148A61L2300/412A61L2430/40A61L2430/02
Inventor 解慧琪苟敏王航江燕林
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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