Low-temperature preservation method and rewarming method of blood vessels

Abstract

The invention discloses a low-temperature preservation method of blood vessels. The low-temperature preservation method comprises the following steps: separating to obtain fresh blood vessel tissues,pouring a cryoprotectant into the blood vessels, putting the blood vessels into a cryoprotectant or a cryoprotectant containing magnetic nanoparticles, putting an obtained mixture into a cryopreservation tube or a cryopreservation bag, sealing, and carrying out programmed cooling; wherein the cryoprotectant comprises the following components 5%-8% of hydroxyethyl starch, 10%-15% of dimethyl sulfoxide, 0.01%-0.02% of a surfactant and the balance a culture medium. The invention also discloses a rewarming method of blood vessels. The rewarming method of blood vessels comprises the following steps: taking out a blood vessel sample cryopreserved by using the cryoprotectant containing the magnetic nanoparticles from liquid nitrogen, placing the blood vessel sample in an alternating magnetic field area, and carrying out magneto-thermal rewarming under the conditions that the magnetic field frequency is 764.8 kHz, the magnetic field intensity is 250-300Gs and the time is 40-60s. According to the blood vessel cryopreservation method, blood vessel breakage or space structure damage caused by different cooling rates of the temperature inside and outside the blood vessels is avoided, the mechanical property of the blood vessels is protected from being affected as far as possible, operation is easy, and efficiency is high.

Description

technical field

[0001] The invention relates to a low-temperature preservation method and a rewarming method for blood vessels, in particular to a deep-low temperature preservation solution for blood vessel tissue, a cooling method and a rewarming method, and belongs to the field of low-temperature medicine. Background technique

[0002] Vascular tissue disease is a clinically common disease, such as vasculitis, phlebitis and arteriosclerosis. Under certain conditions, recanalization of blood vessels is required, and vascular grafting is required when necessary. At present, about tens of millions of people in the world need tissue or organ transplantation to treat cardiovascular diseases every year, and only 15% of them can be cured by repairing their own blood vessels, while the vast majority of patients can only be cured by transplanting other people's normal blood vessels. Blood vessels can cure diseases and restore health. The development of vascular surgery in recent ...

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