Preparation method of immobilized enzyme nanofiber membrane for detecting alcohol content in saliva

A nanofiber membrane, immobilized enzyme technology, applied in immobilized enzymes, biochemical equipment and methods, enzymes and other directions, can solve the problems of large detection errors, inaccurate detection results, increase detection errors, etc., to improve detection sensitivity and precision, improve sensitivity and accuracy, and increase the effect of cycle times
CN103232987BInactive Publication Date: 2015-02-11SOUTHWEST JIAOTONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTHWEST JIAOTONG UNIV
Publication Date
2015-02-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method of an immobilized enzyme nanofiber membrane for detecting the alcohol content in spit. The preparation method comprises the steps of: covalently binding alcohol oxidase through a polymer such as polyethylene glycol or polyethyleneimine as an arm molecular multi-site; further fixing a plurality of subunits of the alcohol oxidase by the polyethyleneimine, polylysine, poly-histidine or chitosan and the like through physical action among charges; then absorbing indigo blue as a chromogenic substrate; generating hydrogen peroxide after ethanol in a sample is oxidized by the alcohol oxidase in detection; leading the indigo blue to fade by the hydrogen peroxide, and conveniently calculating the ethanol concentration in the sample by comparing the fading degree of the indigo blue with a standard color atla. The preparation method is high in sensitivity, simple and convenient to operate, and low in price, can be repeatedly used for a plurality of times, and can be conveniently used for investigating and treating drunk driving by a law-enforcing department; and the reliable evidence is provided for the law enforcement process.
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Description

technical field

[0001] The invention belongs to the technical field of alcohol concentration detection, in particular to a preparation method of an immobilized enzyme nanofiber membrane for detecting alcohol content in saliva. Background technique

[0002] Many industries require qualitative or quantitative detection and analysis of ethanol content. For example, various countries have strict requirements on the blood alcohol content of motor vehicle drivers. In my country, a driver whose blood alcohol concentration reaches 0.2mg / ml is considered to be drunk driving, and when the alcohol concentration reaches 0.8mg / ml, it is considered to be drunk driving. In recent years, tragedies of drunk driving causing tragic accidents are not uncommon. Although the traffic law enforcement department has stepped up law enforcement and punishment, there are always drivers who take risks; in addition, some drivers drive illegally because they cannot accurately judge their own alcohol cont...

Claims

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