Biomimetic specific immune adsorption material with PAMAM (Polyamidoamine) as spacer arm, and preparation method and application thereof

An immunoadsorption material and biomimetic technology, which is applied in the field of preparation of biomimetic specific immunoadsorption materials for blood purification, can solve the problems of protein difficult to sterilize, cannot be coupled to the carrier, and reduce the effect of immunoadsorption, etc., to achieve pro- Good water and blood compatibility, good chemical stability and mechanical strength, avoid expensive effects
CN102671637BActive Publication Date: 2013-10-30SOUTH CHINA UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTH CHINA UNIV OF TECH
Publication Date
2013-10-30

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Abstract

The invention discloses a biomimetic specific immune adsorption material with PAMAM (Polyamidoamine) as a spacer arm, and a preparation method and the application thereof. The preparation process comprises the following steps of: firstly, obtaining sepharose gel azide by reaction of agarose epoxide and sodium azide; preparing a series of PAMAM dendrimer-like polymers in different generations by repeated addition reaction with methyl acrylate and amination reaction with ethidene diamine; after preparing amino acid methyl ester hydrochloride with the amino acid as the raw material, modifying the PAMAM dendrimer-like polymers with the amino acid methyl ester hydrochloride to prepare the amino acid modified PAMAM; coupling the amino acid modified PAMAM with sepharose gel azide through a click reaction so as to obtain the biomimetic specific immune adsorption material with PAMAM as the spacer arm. The material disclosed in the invention is prepared by simple synthetic steps, and the preparation is safe; products have the characteristics of strong specificity, high adsorbing efficiency to the immunoglobulin and excellent regeneration performance, and can be used for IgG (immunoglobulin G) separation and clinical immune adsorption treatment.
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Description

technical field

[0001] The invention belongs to biomedical equipment, and particularly provides a method for preparing a biomimetic specific immunoadsorption material for blood purification. The adsorption material uses a polyamide-amine dendrimer (PAMAM) as a spacer arm. technical background

[0002] It is well known that protein-based immunoadsorbent materials with protein A, G, etc. as ligands have good biospecific affinity for immunoglobulin (IgG). The interaction between protein ligands and IgG involves hydrophobic interaction and electrostatic interaction. Immunoadsorbent materials containing such biospecific ligands have been proven to remove autoantibodies to purify antibodies and treat various autoimmune diseases, so they have been widely used in biological purification and clinical applications. However, protein-based ligands have some disadvantages that cannot be ignored: proteins are difficult to withstand harsh sterilization processes and are easily detached fro...

Claims

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