Microorganism formulation for eliminating nutrilit-resistance function and preparation method

A technology of microbial preparations and anti-nutritional factors, applied in applications, animal feed, animal feed, etc., can solve the problems of inability to guarantee feed intake and feed intake speed, improve feed utilization, ensure the number of viable bacteria, and facilitate operation Effect

Inactive Publication Date: 2009-02-25
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The carrier is white carbon black. This technology is a simple amino acid feed additive formula without any chemical or biological reaction process. The total activity of source enzymes, reducing the effect of stress diarrhea"

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A preparation method of a microbial preparation that eliminates the effect of anti-nutritional factors is:

[0043] The strains and numbers used in the present invention are: Lactobacillus plantarum (ATCC8014), Lactobacillus plantarum, Saccharomyces cerevisiae (IFO0203), Saccharomyces cerevisiae, Bacillus subtilis (1.398) Bacillus subtilis, and the strains are provided by the strain preservation center of the Ministry of Agriculture.

[0044] Step 1: Breeding of Fermentation Strains

[0045] 1. Strain subculture: Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae provided by the Culture Preservation Center of the Ministry of Agriculture are used to subculture through nutrient agar medium to select and breed strains with fast growth and obvious colony characteristics. Preserve strains at ℃ temperature;

[0046] Nutrient agar medium: 3g beef extract, 10g peptone, 5g NaCl, 15-20g agar, 1000ml water, pH7.0-7.2. Sterilize at 121°C for 20min.

[0047] ...

Embodiment 2

[0066] Step 1: Breeding of Fermentation Strains

[0067] 1, bacterial strain passage: with embodiment one;

[0068] 2. Induce the above three strains under the irradiation of ultraviolet rays: make a bacterial suspension from the preserved strains and dilute to 10 -7 , use a UV lamp with a power of 15W at a place of 30cm and a wavelength of 260nm for 20s. The treated bacterial suspension is cultured upside down on a nutrient agar plate at 35°C for 48 hours, and the colony with a fast growth rate is selected for a shake flask experiment. Strain growth rate, all the other are with embodiment one;

[0069] Step 2: Liquid high-speed culture of compound strains

[0070]a. each strain is weighed according to the following percentage by weight: Bacillus subtilis 35%, Lactobacillus plantarum 53%, Saccharomyces cerevisiae 12%;

[0071] b. Liquid culture of lactic acid bacteria and brewer's yeast: mix the Lactobacillus plantarum and brewer's yeast weighed according to the ratio of st...

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Abstract

The invention relates to a microorganism preparation used for eliminating the effect of anti-nutritional factors and is characterized by consisting of the following materials by weight percentage: 28 to 35 percent of bacillus subtilis, 45 to 53 percent of lactobacillus plantarum and 12 to 27 percent of yeast of brewer; a preparation method of the microorganism preparation comprises the following steps: the production of the strain is produced by combining the ray induction and production performance test, the production strain with high yield and large enzyme production are screened, the production technique adopts the technology of liquid single culture and solid composite culture, product accelerators are added into the culture medium so as to lead the product to be rich in active metabolites such as beneficial living bacterium, enzyme, peptide, polysaccharide and amino acids, and the like; after the feed material and complete feed are added with the microorganism preparation, the effect of anti-nutritional factors to the nutrient components in the feed can be eliminated greatly, the palatability of the feed is improved, feed intake is improved, stress resistance and immunity of the cultured animals is enhanced, group uniformity is improved, the growing speed and feed utilization can be improved and the culture cost can be reduced.

Description

technical field [0001] The present invention relates to a microbial preparation for eliminating the effect of anti-nutritional factors and a preparation method thereof, which can significantly eliminate the effect of anti-nutritional factors in common full-price feed or feed raw materials by adding them to full-price feed and feed raw materials, so that the nutrition and nutrition of the feed can be improved. Higher, easier to be eaten, digested and absorbed by livestock and poultry. At the same time, the fermented material is rich in a large number of beneficial live bacteria and active metabolites such as enzymes, peptides, polysaccharides and amino acids, which can improve the immunity of animals, promote the digestion and absorption functions of farmed animals, and improve animal stress. It belongs to the technical field of agricultural biological products . Background technique [0002] Feed is the material basis for the growth of farmed animals. Today, more than 90% o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K10/18
Inventor 江瀚
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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