Microorganism formulation for eliminating nutrilit-resistance function and preparation method
A technology of microbial preparations and anti-nutritional factors, applied in applications, animal feed, animal feed, etc., can solve the problems of inability to guarantee feed intake and feed intake speed, improve feed utilization, ensure the number of viable bacteria, and facilitate operation Effect
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Embodiment 1
[0042] A preparation method of a microbial preparation that eliminates the effect of anti-nutritional factors is:
[0043] The strains and numbers used in the present invention are: Lactobacillus plantarum (ATCC8014), Lactobacillus plantarum, Saccharomyces cerevisiae (IFO0203), Saccharomyces cerevisiae, Bacillus subtilis (1.398) Bacillus subtilis, and the strains are provided by the strain preservation center of the Ministry of Agriculture.
[0044] Step 1: Breeding of Fermentation Strains
[0045] 1. Strain subculture: Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae provided by the Culture Preservation Center of the Ministry of Agriculture are used to subculture through nutrient agar medium to select and breed strains with fast growth and obvious colony characteristics. Preserve strains at ℃ temperature;
[0046] Nutrient agar medium: 3g beef extract, 10g peptone, 5g NaCl, 15-20g agar, 1000ml water, pH7.0-7.2. Sterilize at 121°C for 20min.
[0047] ...
Embodiment 2
[0066] Step 1: Breeding of Fermentation Strains
[0067] 1, bacterial strain passage: with embodiment one;
[0068] 2. Induce the above three strains under the irradiation of ultraviolet rays: make a bacterial suspension from the preserved strains and dilute to 10 -7 , use a UV lamp with a power of 15W at a place of 30cm and a wavelength of 260nm for 20s. The treated bacterial suspension is cultured upside down on a nutrient agar plate at 35°C for 48 hours, and the colony with a fast growth rate is selected for a shake flask experiment. Strain growth rate, all the other are with embodiment one;
[0069] Step 2: Liquid high-speed culture of compound strains
[0070]a. each strain is weighed according to the following percentage by weight: Bacillus subtilis 35%, Lactobacillus plantarum 53%, Saccharomyces cerevisiae 12%;
[0071] b. Liquid culture of lactic acid bacteria and brewer's yeast: mix the Lactobacillus plantarum and brewer's yeast weighed according to the ratio of st...
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