Asexual propagation method of gracilaria lemaneiformis

A technology of vegetative propagation and asparagus, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as insufficient supply of seedlings, and achieve the effects of short induction period, easy operation and simple operation.

Inactive Publication Date: 2017-07-04
QINGDAO QINGQUAN BIOTECH CO LTD
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0003] The present invention aims to provide a method for asexual reproduction of asparagus to solve the problem of insufficient seed supply

Method used

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Embodiment Construction

[0009] The present invention will be described in further detail below in conjunction with specific embodiments.

[0010] A kind of asexual reproduction method of asparagus, the steps are as follows:

[0011] (1) Choose healthy immature asparagus, wash it several times, and then rinse it with sterile seawater to remove the parasitic protozoa and epiphytic algae on the surface of the algae. After culturing with sterile seawater for a week, wash it repeatedly until microscopic examination Soak in 1.5% KI solution for 10 minutes until there are no algae on the surface of the algae, then rinse 3 times with sterilized seawater, soak in 70% alcohol for 10 s, and then rinse 3 times with sterilized seawater;

[0012] (2) Cut the processed asparagus algae into 2-4 mm sections with a scalpel, culture them in PES medium containing 0.25 mg / mL 6-BA, and culture them in the dark for 7 days;

[0013] (3) Change the culture conditions to: temperature 17°C, light intensity 10±2 µE•m −2 •s −...

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Abstract

The invention relates to an asexual propagation method of gracilaria lemaneiformis. The method comprises the following steps: (1) healthy and immature gracilaria lemaneiformis is selected, washed for multiple times, and then flushed with disinfected seawater to remove parasitic protozoans and epiphytic hybrid algas on the surface of the algae body, and after the gracilaria lemaneiformis is cultured for one week by using the disinfected seawater, the cultured gracilaria lemaneiformis is repeatedly washed until the microscopic examination result shows that the surface of the algae body is free of hybrid algas, the washed gracilaria lemaneiformis is soaked in a 1.5% KI solution for 10 minutes, then the soaked gracilaria lemaneiformis is washed with the disinfected seawater for 3 times, the washed gracilaria lemaneiformis is soaked for 10 seconds by using 70% alcohol, and then the soaked gracilaria lemaneiformis is washed with the disinfected seawater for 3 times; (2) the treated gracilaria lemaneiformis algae body is cut into cutting sections of 2-4 mm by using a scalpel blade, and the cut gracilaria lemaneiformis is cultured in a PES culture solution containing 0.25mg / mL 6-BA in dark for 7 days; (3) the culture condition is changed as follows: the temperature is 17 DEG C, the illumination intensity is 10 + / -2 [mu]E.m<-2>.s<-1>, the light cycle is 16h: 8h (Light: Dark). The asexual propagation method is simple and easy to operate and short in induction period, provides a technical method for manual breeding and seedling culture of the gracilaria lemaneiformis, and provides a support for seed production and propagation expanding.

Description

technical field [0001] The invention relates to a method for asexual reproduction of asparagus. Background technique [0002] Asparagus (Gracilaria lemaneiformis) belongs to the genus Gracilaria, which belongs to the class Florideae, the class Gigartinales, and the family Gracilariaceae. It is distributed in all coastal areas of my country. Asparagus is cylindrical and linearly branched. The branches are alternate and partial, and the base is slightly constricted. The base of each plant is a small disc-shaped fixator, and the main branch is thicker than the branch. The algal branches are thick and juicy. The color is reddish-brown, purple-brown, sometimes with green or yellow, and becomes dark brown after drying, and the algal branches shrink. Asparagus is an important economic seaweed, its algal agar content is as high as 30%, and it is one of the important raw materials for making agar and agarin. Currently, as a culture medium for bacteria and microorganisms, agar is ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 褚方强
Owner QINGDAO QINGQUAN BIOTECH CO LTD
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