Method for high-efficiency somatic embryogenesis and plant regeneration method of camellia plants
A technology of somatic embryos and Camellia, which is applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of limiting the speed of plant regeneration and propagation, low proliferation efficiency of somatic embryos, and long time. To achieve the effect of facilitating efficient and rapid regeneration and proliferation, improving proliferation efficiency, and shortening the induction period
Active Publication Date: 2018-11-30
GUANGDONG MINGHUANG TEA
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However, in previous research reports, the time to obtain somatic embryos induced by explants is relatively long, the composition of the medium is relatively complex, or the proliferation efficiency of somatic embryos is low, or the regeneration efficiency of plants is low, which limits the ability of plant regeneration. The speed of regeneration and multiplication makes it difficult for the above-mentioned plant regeneration system to be widely used in the seedling production or genetic engineering of tea trees, camellia oleifera or camellia
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[0022] The present invention will be further described below. It should be noted that the following examples are based on the technical solution and provide detailed implementation and specific operation process, but the protection scope of the present invention is not limited to this example.
[0023] A method for high-efficiency somatic embryogenesis and plant regeneration of Camellia plants, comprising the steps of:
[0024] S1. Somatic embryo induction: Select tea tree, camellia oleifera or red camellia tissue culture seedlings from the preservation medium, cut off 3 / 4 of the leaves, and use the stem segment with 1 / 4 of the leaves as explants, inoculate into somatic embryo induction On the culture medium, cultured at 24-26°C under light conditions, somatic embryos are formed at the stem nodes and petioles; the preservation medium uses MS medium as the basic medium, and adds 20-40g / L sucrose and 8g agar / L, pH is 5.8~6.0; the somatic cell induction medium is based on MS med...
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The invention discloses a method for high-efficiency somatic embryogenesis and plant regeneration of camellia plants, comprising the following steps: excising 3 / 4 leaves of tissue culture seedlings ofcamellia plant tea tree, camellia oleifera or Camellia azalea and using stem segments with 1 / 4 leaf as explants, inoculating onto a somatic embryo induction medium, culturing at 24-26 DEG C under illumination to form somatic embryos at stem nodes and petioles; cutting the somatic embryos into slices of 1-5mm thick, tiling the slices onto a somatic embryo multiplication medium, and culturing at 24-26 DEG C under illumination to form new somatic embryos; transferring the somatic embryos onto a plant regeneration medium, and culturing at 24-26 DEG C under illumination to generate plantlets fromthe somatic embryos. By using the tender stem segments of camellia plants as the explants, a high-efficiency somatic embryogenesis and plant regeneration system is established, thereby effectively improving the tissue culture propagation efficiency of camellia plants. The method provides technical support for seedling production and genetic engineering improvement of crops such as tea trees, camellia oleifera, camellia, etc.
Description
technical field [0001] The invention relates to the technical field of plant induced regeneration, in particular to a method for high-efficiency somatic embryogenesis and plant regeneration of Camellia plants. Background technique [0002] Since ancient times, Camellia plants have been widely used as beverage (tea), edible oil (camellia oil) and ornamental flowers (camellia) in China. The research on tissue culture of Camellia plants began in the 1970s, mainly using stem tips, axillary buds, stem segments, leaves, cotyledons, hypocotyls, embryos and anthers as explants, and through organogenesis, the plant was established. regenerative technology. Due to the low plant regeneration efficiency or multiplication efficiency of these regeneration technology systems, it is still difficult to be widely used in production or genetic engineering. [0003] Since the 1980s, research on plant regeneration through somatic embryogenesis has made some progress in Camellia plants. It is ...
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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 吴华玲于波姜晓辉方开星李红建秦丹丹潘晨东
Owner GUANGDONG MINGHUANG TEA
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