Pear tissue culture seedling rooting and domestication method

Abstract

The present invention relates to a pear tissue culture seedling rooting and domestication method. The method comprises the following steps: 1) rooting culture is conducted: 1.1) after 4 weeks of subculture, strong growing tissue culture seedlings are selected, callus and excess leaves of lower parts of plants are removed, upper half parts of the plants are retained for about 2-3 cm, and the plantsare transferred to a rooting culture medium; and 1.2) 2-3 plants are placed in each bottle, the bottles are placed in a culture room for 4-d dark culture at a culture temperature of 25 + / - 2 DEG C, then light culture is conducted, fluorescent lamps are used as light source, light intensity is 2,000 lx and light cycle is 16 h; 2) cap-opening domestication is conducted: the rooting-cultured tissueculture seedlings begin to take root in about a week and the rooting of the tissue culture seedlings is completed in about 40 d; and during the domestication, bottle caps are first unscrewed, one daylater, the bottle caps are opened for one third, and three days later, the bottle caps are opened two thirds; 3) hydroponic domestication is conducted: after the cap-opening domestication is conductedfor 7 d, the hydroponic domestication is conducted; and 4) substrate transplantation is conducted: when the seedlings grow to 5 cm or more, the seedlings are transplanted into substrates. The pear tissue culture seedling rooting and domestication method can improve rooting rate and is high in transplanting survival rate.

Description

technical field [0001] The invention relates to the technical field of plant planting, in particular to a method for rooting and domesticating pear tissue cultured seedlings. Background technique [0002] Plant in vitro rapid propagation technology is a commonly used method to create new germplasm. The in vitro rapid propagation of Pyrus plants has been widely used, but there are still many shortcomings: pear is a taproot plant, and it is difficult for tissue cultured seedlings to take root. When transplanting, the tissue-cultured seedlings are directly transferred to the substrate for cultivation after opening the cover and acclimating, and the young root system cannot obtain sufficient nutrition from the substrate, resulting in necrosis of the growth point, making it difficult for the tissue-cultured seedlings of Pyrus to take root even if they are successful. applied to production. Contents of the invention [0003] In order to solve the technical problems in the back...

AI Technical Summary

Problems solved by technology

The in vitro rapid propagation of Pyrus plants has been widely used, but there are still many shortcomings: pear is a taproot plant, and it is difficult for tissue cultured seedlings to take root. When transplanting, the tissue-cultured seedlings are directly transferred to the substrate for cultivation after opening the cover and acclimating, and the young root system cannot obtain sufficient nutrition from the substrate, resulting in necrosis of the growth point, making it difficult for the tissue-cultured seedlings of Pyrus to take root even if they are successful. Applied to production

Images