Preservation method of rubber leaves

Abstract

The invention relates to a preservation method of rubber leaves. The method comprises the steps of leaf collecting, paraffin coating, bundling, sealing and casing. By the adoption of the method, rubber leaf samples can be preserved for 5-7 days, leaf cell nucleuses are hardly degraded, the rubber leaf samples meet sample standards of flow cytometry detection, and the purposes of leaf sample transformation and preservation in the flow cytometry detection process of a superior individual in a quite far long-distance rubber forest are addressed. The shelf life of the rubber leaf samples for the flow cytometry detection can be prolonged. Scientific researchers can intensively collect and detect the rubber leaf samples, and thus the round-trip costs in the sampling process are reduced. Moreover, the method has simple requirements for facilities and is simple in operation procedure and relatively low in operation cost.

Description

technical field [0001] The invention belongs to the leaf sample preservation technology of agricultural and forestry economic crops, in particular to a rubber tree leaf sample preservation method, in particular to a rubber tree leaf sample preservation method suitable for long-distance transportation and used for flow cytometry detection. Background technique [0002] rubber tree( Hevea brasiliensis Muell. Arg.) is one of the important tropical economic crops of Euphorbiaceae (Euphorbiaceae), and it is also the only source of large-scale industrialized natural rubber. Hainan and Yunnan provinces in China are the main producing areas of natural rubber in China. Due to the unique physical properties of natural rubber that cannot be replaced by synthetic rubber, it has a wide range of demand in the fields of industry and national defense. With the development of economy and society, the demand for natural rubber is increasingly strong. Imports, the supply situation is more se...

AI Technical Summary

Problems solved by technology

Flow cytometry detection needs to rely on large-scale equipment flow cytometer. This equipment is a precision optical equipment with a large volume and high price. It cannot be carried or frequently transported. For samples in different places, the samples can only be sent to flow The location of the cytometer is used for testing, and the samples tested by flow cytometry must be living samples with complete nucleus structure

The excellent individual rubber trees that need to be screened and identified by flow cytometry are generally located in villages or farms that have been put into production far away from the flow cytometer. The transportation is often inconvenient, and a round trip for leaf sample collection often takes 3 to 3 hours. 5 days, and the climate where the rubber tree is planted is hot, if the rubber tree leaf samples for flow cytometry detection are stored and transported in a conventional way, the leaf samples are often degraded on the way and their nuclei have been degraded, resulting in damage to the leaf samples that cannot be used for detection. It has caused obstacles to the flow cytometry screening, identification and breeding of excellent new varieties of rubber trees

[0004] The transportation and storage of rubber tree leaf samples for flow cytometry detection for more than 3 days cannot be achieved by other leaf sample preservation methods that have been disclosed or reported so far, and no rubber tree leaf samples for flow cytometry detection have been seen yet. Longer-term storage methods, especially rubber tree leaf samples suitable for long-distance transportation for flow cytometry assays

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