Tissue culture rapid breeding technology for notopterygium incisum seeds

Abstract

The invention discloses a tissue culture rapid breeding technology for notopterygium incisum seeds. The tissue culture rapid breeding technology comprises the following steps: inoculating the notopterygium incisum seeds subjected to stratifcation treatment to a basal culture medium to obtain 50 percent or above of induced germination rate; subsequently, transferring buds to a proliferation culturemedium and enabling a proliferation system to be improved to about 6, wherein plants have no malformation or browning; finally, segmenting a proliferation body into a single plant for rooting culture. According to the tissue culture rapid breeding technology disclosed by the invention, the notopterygium incisum seeds are adopted to replace radical buds or young leaves of notopterygium incisum toserve as explants for tissue culture, and the influence of ambient temperature on explant culture is eliminated; in addition, the proliferation efficiency of the explants is remarkably improved, and thereby rapid breeding of notopterygium incisum seedlings is realized.

Description

technical field [0001] The invention relates to a rapid breeding technology of notopterygium seeds through tissue culture, and belongs to the technical field of notopterygium tissue culture and seedling cultivation. Background technique [0002] Notopterygium is a perennial herb of Umbelliferae notopterygii. It uses rhizomes and roots as medicine. It is warm in nature and bitter in taste. It can not only produce sweat and relieve the exterior, but also dispel rheumatism and relieve pain. Arthralgia, joint pain, bruises and other diseases. It is mainly distributed in alpine forest-shaded wetlands in the alpine regions of Sichuan, Qinghai, Gansu, Tibet and other provinces. The seed reproduction ability is low, the growth is slow, and the distribution range is narrow, resulting in a small number of wild plants. In recent years, with the diversification of uses of notopterygium, its market price has continued to rise, resulting in a large amount of excavation, which has brought...

AI Technical Summary

Problems solved by technology

In the above-mentioned prior art scheme, because the root buds or young leaves of notopterygium are used as explants, the tissue induction culture is seriously affected by the low temperature in autumn and winter or the high temperature in summer, and the cultivation period after germination is long and the value-added coefficient is low, thus affecting the quality of notopterygium. Tissue Culture Seedling Quality and Efficiency