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Rhododendron pulchrum callus induction method

A technology of callus induction and callus, which is applied in the field of plant tissue culture, can solve problems such as the establishment of the tissue culture system of Rhododendron splendens, and achieve the effect of increasing the induction rate and high induction rate

Inactive Publication Date: 2019-11-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Rhododendron splendens mainly focuses on physiological changes, hybridization, grafting, landscape utilization, etc. There is no report on the establishment of tissue culture system of Rhododendron splendens

Method used

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  • Rhododendron pulchrum callus induction method
  • Rhododendron pulchrum callus induction method

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Experimental program
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Effect test

Embodiment 1

[0019] 1.1 Experimental materials

[0020] Use the leaves of aseptic seedlings of Rhododendron splendens as explants, and cut the leaves into 2-3 sections to form wounds.

[0021] 1.2 Inoculation

[0022] The experimental material was inoculated in a culture bottle with a medium composition of Anderson medium + 0.5mg / L NAA + 1mg / L Zeatin + 30g / L sucrose + 3.5g / L plant gel, the pH of the medium was 5.5, and the leaf The wound is in contact with the culture medium. When inoculating, wear work clothes, work caps, gloves and masks, and do not speak or breathe into the mouth of plant materials or culture containers. Burn the tweezers and scissors with the outer flame of an alcohol lamp, and set aside after cooling. The culture bottle was sterilized by burning at the outer flame of an alcohol lamp before opening the cap. Hands should not touch the first half of the inoculation instrument (that is, the part that directly cuts and touches the plant material), and hands and arms sh...

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Abstract

The invention discloses a rhododendron pulchrum callus induction method. The method includes the steps of firstly, cutting off a rhododendron pulchrum sterile seedling leaf to form a wound, and usingthe cut leaf as the explant; secondly, inoculating the explant into a culture medium to perform callus primary culture, wherein light intensity during the primary culture is 2000Lux, light cycle during the primary culture is 14 hours, temperature during the primary culture is 25 DEG C, and primary culture time is three weeks; By optimizing the culture medium and selecting culture conditions, the culture medium for inducing rhododendron pulchrum callus is provided, and the culture medium can evidently increasing callus inducing rate. The rhododendron pulchrum callus induction method which is high in inducing rate lays a foundation for the building of a rhododendron pulchrum tissue culture system and genetic transformation system.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a callus induction method of Rhododendron splendens. Background technique [0002] Rhododendron pulchrum is a plant of the genus Rhododendron in the Rhododendron family (Ericaceae). It is cultivated all over the country and is the representative of the rhododendron in the gardening classification of rhododendrons. Its branches and leaves are beautiful, evergreen in all seasons, and its flowers are luxuriant. It can be planted in pots, clusters, group plants in parks or courtyards, or planted as flower hedges on the roadside. Because of its high ornamental value, resistance to pruning, and strong adaptability, Rhododendron splendens has become a common landscaping plant. [0003] In addition to its high ornamental value, Splendid Rhododendron also has high medicinal value. Pharmacological studies have shown that the roots, stems, and leaves of Rhododendron splendens...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 李铮王秀云穆罕默德·蒙奇周泓夏宜平
Owner ZHEJIANG UNIV
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