A Mass Spectrometry Identification Method for Atlantic Salmon and Rainbow Trout

Abstract

The invention discloses a mass spectrometric identification method for Atlantic salmon and rainbow trout, comprising the following steps: a) obtaining the amino acid sequence of Atlantic salmon; b) obtaining the amino acid sequence of rainbow trout; c) using a specific enzymatic hydrolysis method to extract the protein to be detected Cut into small molecular weight polypeptide fragment mixtures, use electrostatic field orbitrap high-resolution mass spectrometry full-scan mode to detect the molecular weight and fragment information of each polypeptide in the mixture, and compare with the previously obtained amino acid sequences to qualitatively determine whether there are Atlantic salmon and rainbow trout d) Select the characteristic peptides qualitatively identified as Atlantic salmon or rainbow trout, and use tandem quadrupole mass spectrometry to establish a multiple reaction monitoring method for quantitative analysis. The invention utilizes the identification of protein characteristic peptides, can realize the qualitative identification and adulteration quantitative research of Atlantic salmon and rainbow trout at the same time, the minimum detection limit can reach 1%, high sensitivity, high accuracy, strong anti-interference ability, and can be widely used Authenticity of Atlantic salmon.

Description

technical field [0001] The invention relates to a protein mass spectrometry detection method, in particular to a mass spectrometry identification method for Atlantic salmon and rainbow trout. Background technique [0002] The authenticity and source assessment of seafood is an important part of food evaluation and identification. Many fish products are mislabeled, and even low-quality fish and counterfeit fish are labeled as high-quality fish, which seriously affects market transparency and damages the health of consumers. A few days ago, the member units of the China Aquatic Products Circulation and Processing Association and the Salmon Branch jointly passed the identification standards of salmon, and designated salmon as a collective name for salmon family fish. Atlantic salmon and rainbow trout are both a type of salmon. This has aroused widespread concern in the industry and doubts from consumers. [0003] Although Atlantic salmon and rainbow trout belong to the family...

AI Technical Summary

Problems solved by technology

The reagents used in PCR-RFLP and AFLP are harmful to the human body, and the operation steps are extremely cumbersome and time-consuming

However, although the identification of species by fluorescent real-time quantitative PCR is fast and accurate, the cost is relatively high.

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