Layer-by-layer self-assembly body having both tumor suppression and bone defect repair functions and preparation method thereof

A layer-by-layer self-assembly and bone defect technology, which is applied in the field of tissue engineering bone repair and biomedical materials, can solve problems such as uneven distribution, denaturation, protein aggregation, etc., and achieve a simple preparation process, avoid environmental pollution, and good stability Effect

Inactive Publication Date: 2018-01-19
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods also have many shortcomings
For example, a large amount of protein is required to exert effective biological functions; cross-linking may cause protein aggregation, denaturation, uneven distribution, etc.

Method used

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  • Layer-by-layer self-assembly body having both tumor suppression and bone defect repair functions and preparation method thereof
  • Layer-by-layer self-assembly body having both tumor suppression and bone defect repair functions and preparation method thereof
  • Layer-by-layer self-assembly body having both tumor suppression and bone defect repair functions and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Preparation of 5β-cholane acidified glycol chitosan (HGC): 0.15g glycol chitosan (GC) and 0.0037g ethyl-dimethylamine-propyl carbodiimide ( EDC) was dissolved in 18ml distilled water, then 0.0045g 5β-cholanic acid (5β-CA) and 0.0022g N-hydroxysuccinimide (NHS) were dissolved in 18ml methanol, and the 5β-CA solution was slowly added dropwise to In the GC solution under stirring state, the solution was stirred at room temperature for 24 hours until the solution became transparent, and dialyzed with a dialysis bag (14KDa) for 3 days, followed by dialysis with 80% methanol, 50% methanol, and pure water for one day, and changed every 3 hours. Once the dialysate, the dialyzed solution is filtered with a 0.8 μm filter membrane, freeze-dried to obtain the white flocculent product (HGC) of 5β-cholane acidified glycol chitosan, and its preparation process is shown in figure 1 .

[0042] (2) Preparation of paclitaxel nanoparticles loaded on 5β-cholane acidified ethylene glyco...

Embodiment 2

[0045] (1) Preparation of 5β-cholane acidified glycol chitosan (HGC): 0.15g glycol chitosan (GC) and 0.0048g ethyl-dimethylamine-propyl carbodiimide ( EDC) was dissolved in 18ml of distilled water, then 0.0060g of 5β-cholanic acid (5β-CA) and 0.0029g of N-hydroxysuccinimide (NHS) were dissolved in 18ml of methanol, and the 5β-CA solution was slowly added dropwise to In the GC solution under stirring state, the solution was stirred at room temperature for 24 hours until the solution became transparent, and dialyzed with a dialysis bag (14KDa) for 3 days, followed by dialysis with 80% methanol, 50% methanol, and pure water for one day, and changed every 3 hours. Dialysate once, the dialyzed solution was filtered with a 0.8 μm filter membrane, and freeze-dried to obtain a white flocculent product (HGC) of 5β-cholaneated glycol chitosan.

[0046] (2) Preparation of paclitaxel nanoparticles loaded on 5β-cholane acidified ethylene glycol chitosan (HGC-PTX): Slowly add paclitaxel PTX...

Embodiment 3

[0049] (1) Preparation of 5β-cholane acidified glycol chitosan (HGC): 0.15g glycol chitosan (GC) and 0.0074g ethyl-dimethylamine-propyl carbodiimide ( EDC) was dissolved in 18ml of distilled water, then 0.0090g of 5β-cholanic acid (5β-CA) and 0.0045g of N-hydroxysuccinimide (NHS) were dissolved in 18ml of methanol, and the 5β-CA solution was slowly added dropwise to In the GC solution under stirring state, the solution was stirred at room temperature for 24 hours until the solution became transparent, and dialyzed with a dialysis bag (14KDa) for 3 days, followed by dialysis with 80% methanol, 50% methanol, and pure water for one day, and changed every 3 hours. Dialysate once, the dialyzed solution was filtered with a 0.8 μm filter membrane, and freeze-dried to obtain a white flocculent product (HGC) of 5β-cholaneated glycol chitosan.

[0050] (2) Preparation of paclitaxel nanoparticles loaded on 5β-cholane acidified ethylene glycol chitosan (HGC-PTX): Slowly add paclitaxel PTX...

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Abstract

The invention discloses a layer-by-layer self-assembly body having both tumor suppression and bone defect repair functions and a preparation method thereof, and belongs to the field of biomedical materials. In the invention, by means of the layer-by-layer self-assembly technology, a micron / nano-scale biphasic calcium phosphate (BCP) interface is formed, in a layer-by-layer alternating manner, through bionic modification of an adhesion and osteogenesis promoting anionic fusion protein (recombinant fibronectin / cadherin, rFN-CDH) and contains 5[beta]-cholanic acid ethylene glycol chitosan cationic nano-particles supporting an antitumor medicine paclitaxel (HGC-PTX). The structure has physical and chemical properties such as excellent biocompatibility and excellent hydrophilicity, maintains the anti-cancer effect of the HGC-PTX nano-particles and the excellent adhesion and osteogenesis activity promoting of the rFN-CDH protein, so that the layer-by-layer self-assembly body may be used as atumor suppression and bone defect repair material and has a bright future.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to the field of tissue engineering bone repair. Background technique [0002] Bone is one of those tissues that is difficult to regenerate in adults, and certain traumas, tumors, infections that suggest osteonecrosis force the surgeon to remove the affected bone, creating a gap and eliminating the damaged tissue. Osteosarcoma is a malignant tumor that occurs in adolescents and seriously affects individuals and society. It has poor differentiation, early metastasis, and high disability and death rate. Although there are various treatment methods such as radiotherapy / chemotherapy, bone resection of tumor segment + inactivation and replantation, tumor-type joint prosthesis, and even amputation, the long-term effect of treatment is still not optimistic. Statistics show that even after aggressive adjuvant chemotherapy and surgery, the 5-year survival rate after surgery is only 60-70%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K47/65A61K47/52A61K47/61A61K31/337A61K38/39A61K38/17A61P35/00A61P19/08A61K33/24A61K31/704
Inventor 葛亮任圆朱颖
Owner CHINA PHARM UNIV
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