Calcium ion binding protein derived from stem nodule as well as encoding gene and application thereof

Abstract

The invention discloses a calcium ion binding protein derived from stem nodule as well as an encoding gene and application thereof. The calcium ion binding protein gene BjCBP1 has a base sequence as shown in SEQ NO.1; the constructed recombinant prokaryotic expression vector pET32a-BjCBP1 consists of a sequence SEQ ID NO.1 and a prokaryotic expression vector pET32a(+); the recombinant expression vector pCAMBIA1302-BjCBP1 consists of the sequence SEQ ID NO.1 and a plant expression vector pCAMBIA 1302; the plant expression vector is used for plant genetic transformation; the BjCBP1 genes are subjected to over-expression under the start of a CaMV35S promoter, and lots of BjCBP1 proteins are synthesized, so that the adverse situation resistance of plants is improved.

Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular to a calcium ion-binding protein derived from stem mustard and its coding gene and application. Background technique

[0002] Calcium ion signaling plays an important role in many cellular activities, such as cell metabolism, gene expression, cytoskeleton dynamic changes, cell cycle, cell death, signal transduction and other processes.

[0003] In plant cells, one of the functions of calcium ions is as a second messenger. Many external environmental signals, such as light, biotic stress, abiotic stress, plant hormones, etc., will stimulate the Ca in the cytosol. 2+ Concentration increases, thereby triggering calcium ion signaling. The first responder in the calcium ion signaling pathway is the calcium ion binding protein, which binds Ca 2+ A later conceptual change exposes the hydrophobic face, thereby activating or inhibiting the activity of other proteins, thereby initiating s...

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