A kind of Penicillium phospholyticum psf and its application
A technology of Penicillium phospholyticum and Penicillium, which is applied in the field of Penicillium phospholyticum PSF strains, can solve the problems of rapid degradation of phosphorus-dissolving ability, high strain elimination rate, and poor colonization ability, so as to increase the content of soluble phosphate, Resolves phosphorus deficiency, good growth and colonization effects
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Embodiment 1
[0031] A kind of Penicillium phosphatidylase PSF provided by the invention
[0032] 1. Origin of the strain
[0033] The sample is from the salinized soil of the Yellow River Delta;
[0034] Selected from the salinized soil of the Yellow River Delta, weighed 10 g of soil sample and placed it in 90 mL of sterile water, and shaken it for 3 minutes at a rotational speed of 3000 rpm to prepare a soil suspension;
[0035] 2. Isolation of strains
[0036] Dilute the soil suspension by a 10-fold gradient, and then pipette 10 -4 , 10 -5 , 10 -6 The three gradients of soil suspension were 200 μL, respectively, coated on the PVK inorganic phosphorus solid medium, and then the inoculated PVK inorganic phosphorus solid medium was inverted in a biochemical incubator, and incubated at 28 °C for 7 days. 15 strains of phosphate-dissolving fungi were screened out, among which the strain number is PSF with the strongest ability to dissolve phosphates, and the phosphate-dissolving circle fo...
Embodiment 2
[0049] Solubilization of Penicillium phosphatidylase PSF on various insoluble phosphates
[0050] This strain mainly targets four insoluble phosphates, namely Ca 5 (PO 4 ) 3 OH, AlPO 4 , Zn 3 (PO 4 ) 2 and FePO 4 Phosphorus solubilization;
[0051] Preparation of Penicillium phosphate solubilization PSF bacterial suspension: according to the volume fraction ratio, add 5 parts of PBS buffer to 1 part of Penicillium phosphate solubilization PSF, and mix evenly to prepare Penicillium phosphate solubilization PSF bacterial suspension, which is for later use;
[0052] Ca5(PO 4 )3OH, AlPO 4 , Zn 3 (PO 4 ) 2 , FePO 4 Take the preparation of Ca5(PO4)3OH phosphate detection solution as an example:
[0053] Add 50mL PVK liquid medium, 0.5g Ca to a 150mL Erlenmeyer flask 5 (PO 4 ) 3 OH, 500 μL Penicillium phosphatidylase PSF bacterial suspension, under the condition of temperature of 28 ℃, with a rotating speed of 200 r / min shaker for 5-7 d, to obtain Ca 5 (PO 4 ) 3 O...
Embodiment 3
[0060] Preparation of fermentation broth of Penicillium phosphatidylsis PSF
[0061] Penicillium phosphatidylsis PSF was inoculated on PDA medium, cultured at 28°C for 3 days, and the agar block containing PSF mycelium with a diameter of 7 mm was inoculated by punching method and placed in PVK liquid medium at a temperature of 28°C and shaken. The rotation speed was 180 rpm, and the shake flask was fermented for 96 hours to obtain the Penicillium phosphatidylase PSF fermentation broth, which was used for later use.
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