Space-induced efficient bifidobacterium longum, application thereof, and preparation method of capsule preparation thereof

Abstract

The invention relates to space mutagenesis of high-efficiency bifidobacterium longum, its application and a preparation method of capsule preparation. Bifidobacterium is a strict anaerobic bacteria. It is difficult to produce live bacteria preparations, and because the live bacteria are sensitive to acid, alkali and heat, it is easy to cause inactivation during storage and oral administration. The invention provides a strain of Bifidobacterium longum S7-T6, which can be applied to the preparation of medicines and health care products for regulating intestinal flora balance and treating diarrhea. The Bifidobacterium longum of the present invention has enhanced oxygen resistance and salt resistance, and can be stably inherited to 30 generations without degeneration; it can grow under the culture conditions with a content of 6% NaCl; the methyl red test and the VP test are positive, indicating that Its acid production ability is improved. It has been clinically verified that its live bacteria preparation can promote digestion, effectively regulate the balance of intestinal flora, and can be used to prepare health care products or medicines.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of high-efficiency Bifidobacterium longum induced by space mutation, its application and a preparation method of a capsule preparation. Background technique [0002] Bifidobacterium longum, Latin scientific name Bifidobacterium longum , belonging to the genus Bifidobacterium, Bifidobacterium Orla-Jensen is an anaerobic Gram-positive bacillus isolated from the feces of breast-fed infants by the French scholar Tissier in 1899. The ends are often forked, so named Bifidobacteria. With the rise of microecology and the progress of the medical revolution in the past two decades, the importance of bifidobacteria research has been increasingly recognized. [0003] Bifidobacterium is a physiological bacterium in the human gastrointestinal tract. It is a beneficial bacterium. It is inseparable from the health of the human body. The most common distribution is in breast-fed infa...

AI Technical Summary

Problems solved by technology

Although "replenishment of live bacteria in vitro" avoids the unsafe problems brought about by the method of "proliferation of live bacteria in vivo", bifidobacteria are a strict anaerobic bacteria, and the production of live bacteria preparations is difficult, and due to the impact of live bacteria on Sensitive to acids, bases, and heat, making preservation and oral inactivation prone

At the same time, the mutant strains of bifidobacterium induced by conventional mutagenesis are unstable and the yield is not high, which causes greater difficulties in the production of live bacterial preparations

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