Space-induced efficient bifidobacterium longum, application thereof, and preparation method of capsule preparation thereof

Abstract

The invention relates to space-induced efficient bifidobacterium longum, an application thereof, and a preparation method of a capsule preparation thereof, wherein bifidobacterium longum is a strict anaerobion, and the live bacterium preparation of bifidobacterium longum is difficult to produce, and is easily inactivated during preservation and oral administration because live bacteria are sensitive to acid, alkali and heat. The invention provides bifidobacterium longum S7-T6, which is capable of being applied to preparation of medicines and healthcare products for regulating intestinal flora balance and treating diarrhea. The bifidobacterium longum disclosed by the invention has enhanced oxygen-resistant characteristic and salt-resistant characteristic, and can be stably inherited to 30 generations without degeneration and is capable of growing on a culture condition of NaCl content is 6%; results of methyl red experiments and VP (Voges-Proskauer) experiments are positive, which indicates that the acid production capacity of bifidobacterium longum is improved; and through clinical verifications, the live bacterium preparation of bifidobacterium longum is capable of promoting digestion, effectively regulating intestinal flora balance and can be used for preparing healthcare products or medicines.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of high-efficiency Bifidobacterium longum induced by space mutation, its application and a preparation method of a capsule preparation. Background technique [0002] Bifidobacterium longum, Latin scientific name Bifidobacterium longum , belonging to the genus Bifidobacterium, Bifidobacterium Orla-Jensen is an anaerobic Gram-positive bacillus isolated from the feces of breast-fed infants by the French scholar Tissier in 1899. The ends are often forked, so named Bifidobacteria. With the rise of microecology and the progress of the medical revolution in the past two decades, the importance of bifidobacteria research has been increasingly recognized. [0003] Bifidobacterium is a physiological bacterium in the human gastrointestinal tract. It is a beneficial bacterium. It is inseparable from the health of the human body. The most common distribution is in breast-fed infa...

AI Technical Summary

Problems solved by technology

Although "replenishment of live bacteria in vitro" avoids the unsafe problems brought about by the method of "proliferation of live bacteria in vivo", bifidobacteria are a strict anaerobic bacteria, and the production of live bacteria preparations is difficult, and due to the impact of live bacteria on Sensitive to acids, bases, and heat, making preservation and oral inactivation prone

At the same time, the mutant strains of bifidobacterium induced by conventional mutagenesis are unstable and the yield is not high, which causes greater difficulties in the production of live bacterial preparations

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