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Method for activating oil reservoir indigenous microorganisms to produce object surface active agents

A technology of endogenous microorganisms and surfactants, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganism measurement/testing, etc., to achieve the effects of wide application range, strong operability and strong pertinence

Inactive Publication Date: 2014-03-12
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the literatures that have been reported so far use the total number of bacteria activated by endogenous microorganisms as one of the main indicators, or qualitatively and quantitatively describe the changes in physical and chemical properties of crude oil after activating endogenous microorganisms, such as emulsification and dispersion, and lack of biological surfactants produced by endogenous microorganisms. direct and targeted research

Method used

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  • Method for activating oil reservoir indigenous microorganisms to produce object surface active agents
  • Method for activating oil reservoir indigenous microorganisms to produce object surface active agents
  • Method for activating oil reservoir indigenous microorganisms to produce object surface active agents

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Experimental program
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Embodiment 1

[0027] The burial depth of this reservoir is 1240m~1360m, the formation temperature is 60℃, the average porosity is 31.3%, and the average permeability is 989×10 -3 μm 2 , the original oil saturation is 62%. Utilize the method for activating oil reservoir endogenous microorganisms of the present invention to produce biosurfactant to carry out microbial flooding pilot test, concrete steps are as follows:

[0028] 1. On-site sampling, analysis of microbial and oil-water physical and chemical properties of biosurfactants produced by reservoir samples

[0029] One water injection well and two production wells corresponding to the clear injection-production relationship in this block were sampled, and the reservoir samples were subjected to selective microbial culture and quantitative analysis of functional genes. The results are shown in Table 1.

[0030] Table 1 Selective culture and quantitative analysis of functional genes of reservoir samples in block Z1

[0031]

[0032...

Embodiment 2

[0068] The burial depth of this reservoir is 938m~1027m, the formation temperature is 49℃, the average porosity is 28.5%, and the average permeability is 1212×10 -3 μm 2 , the original oil saturation is 68%. Utilize the method of the present invention to activate the endogenous microorganism of oil reservoir to produce biosurfactant to carry out microbial flooding test, concrete steps are as follows:

[0069] 1. On-site sampling, analysis of microbial and oil-water physical and chemical properties of biosurfactants produced by reservoir samples

[0070] One water injection well and two production wells corresponding to the clear injection-production relationship in this block were sampled, and the reservoir samples were subjected to selective microbial culture and quantitative analysis of functional genes. The results are shown in Table 8.

[0071] Table 8 Selective culture and quantitative analysis of functional genes of reservoir samples in Block T7

[0072]

[0073] T...

Embodiment 3

[0095] The burial depth of this reservoir is 2020-2040m, the formation temperature is 95℃, the average porosity is 30.8%, and the average permeability is 315×10 -3 μm 2 , the original oil saturation is 56%. Utilize the method for activating oil reservoir endogenous microorganisms to produce biosurfactant of the present invention to carry out microbial oil displacement test, concrete steps are as follows:

[0096] 1. On-site sampling, analysis of microbial and oil-water physical and chemical properties of biosurfactants produced by reservoir samples

[0097] One injection well and two production wells corresponding to clear injection-production relationship in this block were sampled, and each sample was 5L. Selective culture of microorganisms and quantitative analysis of functional genes were carried out on the reservoir samples, and the results are shown in Table 12.

[0098] Table 12 Selective culture and functional gene quantitative analysis of reservoir samples in block...

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Abstract

The invention belongs to the technical field of microbial enhanced oil recovery, and particularly relates to a method for selectively activating water-drive oil reservoir indigenous microorganisms to produce object surface active agents so as to improve the crude oil recovery efficiency. The method includes the following steps of firstly, conducting sampling on site, analyzing the microorganisms which are contained in an oil reservoir sample and produce the object surface active agents, and analyzing the oil-water physicochemical property; secondly, determining dominant population capable of producing the object surface active agents and key metabolic substrates of the dominant population, and designing an active agent formula system; thirdly, optimizing an active agent formula; fourthly, optimizing the active agent on-site injection technology; fifthly, conducting a test on site. The method has the advantages of being high in pertinence and reliability, wide in oil reservoir application range, simple in method, high in operability, good in on-site implementing effect and the like, and therefore the method can be widely applied to microorganism oil displacement on-site tests.

Description

technical field [0001] The invention belongs to the technical field of microbial oil recovery, in particular to a method for enhancing oil recovery by selectively activating surfactants produced by endogenous microorganisms in water flooding oil reservoirs. Background technique [0002] The oil displacement effect of biosurfactants is one of the main mechanisms of microbial oil recovery. At present, the application of biosurfactants in microbial oil recovery technology mainly adopts the method of injecting surface fermentation products (surface method) and exogenous bacterial species (exogenous microbial oil flooding) into the reservoir. The high cost of surface fermentation is one of the main factors restricting its large-scale application, while exogenous microbial flooding still has the problem of the adaptability of bacteria to the reservoir environment. Relatively speaking, the endogenous microbial flooding technology utilizes the massive growth and reproduction of the...

Claims

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Application Information

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IPC IPC(8): E21B43/22C09K8/584C12Q1/68C12Q1/64C12Q1/06C12R1/385
Inventor 高光军宋永亭郭辽原宋欣王静谭晓明林军章李彩风曹嫣镔郭省学汪卫东
Owner CHINA PETROLEUM & CHEM CORP
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