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Method for improving survival rate of initial culture of passiflora caerulea

A technology for the primary culture of Passiflora, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low survival rate, pollution rate, and high browning mortality rate of the primary culture, and achieve improved survival rate, browning The effect of lowering the mortality rate and reducing the browning rate

Pending Publication Date: 2020-05-08
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of method that improves Passiflora primary culture survival rate, thus overcomes the high pollution rate, browning death rate in Passiflora tissue culture process, the shortcoming that primary generation culture survival rate is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for improving the survival rate of the primary culture of passionflower, including the following steps:

[0028] (1) Preparation of primary culture medium: The formulation of primary culture medium is MS basic medium, 6-BA 1.0mg / L, IBA0.1mg / L, ascorbic acid 0.01g / L, hydrolyzed casein 0.3g / L, sucrose 30g / L, agar 6g / L, adjust the pH of the medium to 5.8-6.0, and separate them into triangular flasks, each with 20 bottles per liter, a total of 100 bottles, and a cotton stopper on the top of the bottle, wrap a layer of kraft paper and use string Fasten it firmly, sterilize it in an autoclave at 121°C for 20 minutes, take it out and cool it for later use.

[0029] (2) Cultivation of explants: select strong-growing passionflower branches from the field, cut the branches into stem segments containing 1-2 buds, cut off 2 / 3-3 / 4 of the leaves, and cut them in the greenhouse. In the nursery pot with a diameter greater than 20cm, spray a sterilization, insecticide and virus pr...

Embodiment 2

[0037] A method for improving the survival rate of the primary culture of passionflower, including the following steps:

[0038] (1) Preparation of primary culture medium: The formulation of primary culture medium is: MS basic medium, 6-BA 1.0mg / L, IBA0.1mg / L, ascorbic acid 0.01g / L, hydrolyzed casein 0.3g / L, sucrose 30g / L, agar 6g / L, adjust the pH of the medium to 5.8-6.0, and separate them into triangular flasks, each 20 bottles per liter, 100 bottles in total, stopper with a cotton stopper, wrap a layer of kraft paper and tie with string Firmly, sterilize in an autoclave at 121°C for 20 minutes, take it out and cool it for later use.

[0039] (2) Cultivation of explants: select strong-growing passionflower branches from the field, cut the branches into stem segments containing 1-2 buds, cut off 2 / 3-3 / 4 of the leaves, and cut them in the greenhouse. In the nursery pot with a diameter greater than 20cm, spray the cuttings with sterilization, insecticide and virus prevention and c...

Embodiment 3

[0044] A method for improving the survival rate of the primary culture of passionflower, including the following steps:

[0045] (1) Preparation of primary culture medium: The formulation of primary culture medium is MS basic medium, 6-BA 1.0mg / L, IBA0.1mg / L, ascorbic acid 0.01g / L, hydrolyzed casein 0.3g / L, sucrose 30g / L, agar 6g / L, adjust the pH of the medium to 5.8-6.0, and separate them into triangular flasks, each 20 bottles per liter, 100 bottles in total, stopper with a cotton stopper, wrap a layer of kraft paper and tie with string Firmly, sterilize in an autoclave at 121°C for 20 minutes, take out and cool for later use.

[0046] (2) Disinfection of explants: select strong-growing branches from the plants cultivated in the field, cut off the leaves, soak in 2% detergent solution for 15 minutes, rinse with running water for 30 minutes, and soak in 1000 times liquid carbendazim 10min, cut the branches into small sections of 6-8cm, the axillary bud is located in the middle o...

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Abstract

The invention discloses a method for improving a survival rate of the initial culture of passiflora caerulea. The method comprises the following steps: (1) initial culture medium preparation; (2) explant cultivation: selecting passiflora caerulea branches, cutting the branches into stem segments containing 1-2 buds, cutting off part of leaves, performing cuttage into a seedling-raising pot, performing placing into a greenhouse, spraying sterilization, desinsection and virus prevention and control medicines once during cuttage, spraying the sterilization, desinsection and virus prevention and control medicines once every 5-10 days, and taking the branches as explants for tissue culture after cultivation for a period of time; (3) explant disinfection: cutting leaves of the branches, cuttingthe branches into multiple segments, wherein axillary buds are located in the middle of the stem segments, and performing disinfection with a disinfectant containing HgCl2 and ascorbic acid; and (4) explant inoculation and culture. In the method, an explant material is cultured, the material is placed in the greenhouse for culture, pre-sterilization and other treatments are performed, the cleanliness of the explants is improved, and an HgCl2 disinfection time is shortened, so that a pollution rate of the initial culture is reduced to 15% or less. A browning rate of the explants is reduced, anda survival rate is increased.

Description

Technical field [0001] The invention relates to the technical field of crop tissue culture, in particular to a method for improving the survival rate of the primary culture of passionflower. Background technique [0002] Passionflower is a perennial evergreen woody vine of the Passiflora genus of the Passiflora family. It is native to South America from Brazil to Argentina. It is mainly distributed in Taiwan, Guangdong, Fujian, Guangxi, Yunnan and other provinces in China. It is edible , Medicinal, oil and greening and ornamental in one, high economic value. The juice content of the passion fruit fruit accounts for 30%-40% of the fruit weight. It is rich in 17 kinds of amino acids, organic acids and VC, SOD, phosphorus, calcium, iron, potassium, protein and other nutrients necessary for the human body. Nutritional value. At present, the expansion and propagation of passionflower in our country mainly use seedlings and vine cuttings. There are many problems in production, such a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘洁云牟海飞蔡玲黄永才吴艳艳黄伟华田青兰彭嘉宇张英俊韦邵龙韦弟
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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